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Sains Malaysiana 52(1)(2023):
http://doi.org/10.17576/jsm-2023-5201-14
Evaluation of Xylose-Utilising Yeasts for Xylitol Production from Second-Generation Ethanol Vinasse and Effect of Agitation Intensity in Flask-Scale Xylitol Production (Penilaian Yis
mengguna Xilosa untuk Pengeluaran Xilitol daripada Vinasse Etanol Generasi
Kedua dan Kesan Keamatan Pergolakan dalam Pengeluaran Xilitol Skala Flask)
SREYDEN HOR1,2,
MALLIKA BOONMEE KONGKEITKAJORN2,3,* &
ALISSARA REUNGSANG2,4
1Graduate
School, Khon Kaen University, Khon Kaen,
Thailand
2Department of
Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand
3Research Center for Environmental and Hazardous Substance Management
(EHSM), Khon Kaen University, Khon Kaen,
Thailand
4Academy of
Science, Royal Society of Thailand, Bangkok, Thailand
Received: 5 June
2022/Accepted: 5 October 2022
Abstract
This study aimed to select a yeast strain that
effectively utilises xylose to produce xylitol from the vinasse of ethanol broth obtained from the fermentation of sugarcane bagasse
hydrolysate. Eleven strains of xylose-fermenting yeasts were evaluated for
their abilities to utilise xylose and produce xylitol. Two strains that showed
outstanding performance in the semi-defined xylose medium were selected for
further testing with a vinasse medium. Candida guilliermondii TISTR 5068 showed a superior xylitol
production of 7.03 ± 0.08 g/L with the xylitol yield of 0.70 g/gxylose when cultured in bagasse-based ethanol vinasse. The strain was further tested for its xylitol
production performance when cultured at four different agitation intensities.
Excessive agitation resulted in a rapid xylitol production rate but caused
xylitol consumption once the xylose was depleted. Moderate agitation resulted
in the highest xylitol yield of 0.79 g/gxylose.
The results of this study have provided important information for the
development of the xylitol production process using waste streams from cellulosic ethanol production.
Keywords: Aeration; biorefineries;
fermentation; vinasse; xylitol; yeasts
Abstrak
Kajian
ini bertujuan untuk memilih strain yis yang menggunakan xilosa secara berkesan
untuk menghasilkan xilitol daripada vinasse rebusan etanol yang diperoleh
daripada penapaian hidrolisat hampas tebu. Sebelas
strain yis difermentasi xilosa dinilai untuk kebolehan mereka untuk menggunakan
xilosa dan menghasilkan xilitol. Dua
strain yang menunjukkan prestasi cemerlang dalam medium xilosa separa takrif
telah dipilih untuk ujian selanjutnya dengan medium vinasse. Candida guilliermondii TISTR
5068 menunjukkan pengeluaran unggul xilitol sebanyak 7.03 ± 0.08 g/L dengan
hasil xilitol sebanyak 0.70 g/gxylose apabila dikultur dalam vinasse etanol berasaskan hampas. Strain
diuji lagi untuk prestasi pengeluaran xilitol apabila dikultur pada empat
keamatan pengadukan yang berbeza. Penggoncangan
yang berlebihan mengakibatkan kadar pengeluaran xilitol yang cepat tetapi
menyebabkan penggunaan xilitol sebaik sahaja xilosa habis. Penggoncangan
sederhana menghasilkan hasil xilitol tertinggi iaitu 0.79 g/gxylose. Hasil
kajian ini telah memberikan maklumat penting untuk pembangunan proses
penghasilan xilitol menggunakan aliran sisa daripada penghasilan etanol
selulosa.
Kata kunci: Fermentasi; kilang penapisan bio; pengudaraan; vinasse; xilitol; yis
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*Corresponding author; email: mallikab@kku.ac.th
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