Sains Malaysiana 52(6)(2023): 1649-1670

http://doi.org/10.17576/jsm-2023-5206-04

Persicaria minor F-box Gene PmF-box1 Indirectly Affects Arabidopsis thaliana LOX-HPL Pathway for Green Leaf Volatile Production

(Gene F-box Persicaria minor PmF-box1 Secara Tidak Langsung Mempengaruhi Tapak Jalan LOX-HPL Arabidopsis thaliana untuk Penghasilan Sebatian Meruap Daun Hijau)

NUR-ATHIRAH ABD-HAMID¹, MUHAMMAD NAEEM-UL-HASSAN^2,3, ZAMRI ZAINAL^1,2& ISMANIZAN ISMAIL^1,2*

¹Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

²Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Department of Chemistry, University of Sargodha, Sargodha, Punjab 40100, Pakistan

Received: 3 October 2022/Accepted: 6 June 2023

Abstract

Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions.

Keywords: F-box proteins; hydroperoxide lyase; Kelch-repeats; lipoxygenase; oxylipin

Abstrak

Sebatian meruap daun hijau (GLV) memainkan peranan penting dalam pertahanan tumbuhan, interaksi tumbuhan-tumbuhan dan interaksi tumbuhan-serangga. Tumbuhan membebaskan GLV serta merencat pertumbuhan dan propagasi patogen tumbuhan. Dalam kajian ini, pengekspresan lampau PmF-box1 dalam A. thaliana jenis liar telah menunjukkan pengawalaturan menurun gen yang terlibat dalam tapak jalan lipoksigenase-hidroperoksid liase (LOX-HPL) yang menyumbang kepada biosintesis GLV. Ia mengakibatkan pengurangan penghasilan heksanal yang ketara dalam tumbuhan yang mengekspres PmF-box1 secara melampau. Corak pengekspresan gen cabang LOX-HPL dalam tumbuhan yang mengekspres PmF-box1 secara melampau dengan ulangan Kelch (KMF) yang terubah suai menunjukkan corak pengekspresan yang hampir sama dengan gen cabang LOX-HPL di dalam tumbuhan kawalan vektor (VC). Ini menunjukkan bahawa jujukan protein KMF yang berfungsi tidak bertanggungjawab terhadap penurunan yang signifikan bagi semua GLV termasuk heksanal, 1-heksanol, (Z)-3-hexen-1-ol dan karbon 5 (C5) meruap, 1-penten-3-ol, di dalam tumbuhan yang mengekspreskan KMF secara melampau. Tambahan pula, kajian ini juga menunjukkan bahawa perkadaran relatif penghasilan 1-penten-3-ol kepada heksanal adalah lebih tinggi di dalam tumbuhan yang mengekspres PmF-box1 secara melampau. Berdasarkan carian perbandingan kepustakaan semasa, PmF-box1 didapati tidak dapat berinteraksi secara langsung dengan protein atau faktor transkripsi bagi tapak jalan LOX-HPL. Sebaliknya, PmF-box1 berinteraksi dengan SAMS1, yang kemudiannya mempengaruhi gen enzim tapak jalan HPL. Oleh itu, kajian ini menunjukkan PmF-box1 berpotensi berperanan dalam memanipulasi penghasilan GLV.

Kata kunci: Hidroperoksid liase; lipoksigenase; oksilipin; protein F-box; ulangan Kelch

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*Corresponding author; email: maniz@ukm.edu.my