Sains Malaysiana 39(5)(2010): 731–735

Determination of Aflatoxins B1 and B2 in Peanuts and Corn Based Products

(Penentuan Aflatoksin B1 dan B2 dalam Kacang Tanah dan Produk Jagung)

Loh Saw Hong*, Nur Iryani Mohd Yusof & How Mun Ling

Department of Chemical Sciences, Faculty of Science and Technology

Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia

Diserahkan: 19 November 2008 / Diterima: 8 Februari 2010

ABSTRACT

A method for the determination of aflatoxins B1 and B2 in peanuts and corn based products is described. The samples were extracted with a mixture of acetonitrile-water (84:16), followed by multifunctional clean-up and liquid chromatography with fluorescence detection. Both calibration curves showed good correlation from 4.0 to 32.0 ppb for aflatoxin B1 (r=0.9999) and 1.2 to 9.6 ppb for aflatoxin B2 (r=0.9997). The detection limit of aflatoxins B1 and B2 were established at 0.1 and 0.03 ppb, respectively, based on signal-to-noise ratio of 3:1. Average recoveries for the determination of aflatoxins B1 and B2 at 10 and 3 ppb spiking levels, respectively ranged from 94.2 to 107.6%. A total of 20 peanut samples and corn based products were then obtained from retail shop and local market around Kuala Terengganu and analyzed for aflatoxins B1 and B2 contents, using the proposed method. Aflatoxins B1 and B2 were detected in 5 out of the 9 peanuts samples and 5 out of the 11 corn based products, at levels ranging from 0.2 to 101.8 ppb.

Keywords: Aflatoxins; corns; multifunctional clean-up column; peanuts

ABSTRAK

Satu kaedah penentuan aflatoksin B1 dan B2 dalam kacang tanah dan produk jagung dihuraikan. Sampel diekstrak dengan menggunakan campuran asetonitril-air (84:16), diikuti dengan proses pembersihan dengan turus pelbagai fungsi dan pemisahan melalui kromatografi cecair dengan pengesan pendarfluor. Kedua-dua lengkuk penentukuran menunjukkan kolerasi yang baik daripada 4.0 hingga 32.0 ppb untuk aflatoksin B1 (r=0.9999) dan 1.2 hingga 9.6 ppb untuk aflatoksin B2 (r=0.9997). Had pengesanan ditentukan masing-masing pada 0.1 dan 0.03 ppb untuk aflatoksin B1 dan B2, berdasarkan nisbah isyarat-bisingan 3:1. Purata perolehan semula pada aras pemakuan 10 dan 3 ppb masing masing untuk aflatoksin B1 dan B2 adalah dalam julat 94.2 hingga 107.6%. Jumlah sebanyak 20 sampel kacang tanah dan produk jagung kemudian diperolehi dari kedai runcit dan pasaraya tempatan di sekitar Kuala Terengganu untuk dianalisis kehadiran aflatoksin B1 dan B2, dengan menggunkan kaedah yang dicadangkan. Aflatoksin B1 dan B2 telah dikesan dalam 5 daripada 9 sampel kacang tanah dan 5 daripada 11 produk jagung, pada kepekatan dalam julat 0.2 hingga 101.8 ppb.

Kata kunci: Aflatoksin; jagung; kacang tanah; pembersihan dengan turus pelbagai fungsi

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*Pengarang untuk surat-menyurat; email: lohsh@umt.edu.my