Sains Malaysiana 41(4)(2012): 419-422

Challenges in Culturing Macaca fascicularis Bone Marrow Stem Cells

(Cabaran di dalam Pengkulturan Sel Stem Sum-Sum Tulang Macaca fascicularis)

S. Sharen @ Sharen Aini, M.H. Ng, S.B. Shamsul & B.H.I. Ruszymah

Tissue Engineering Centre, Faculty of Medicine

Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff

Bandar Tun Razak Cheras, 56000 Kuala Lumpur, Malaysia

R. Masfueh & B. Badiah

Department of Periodontology, Faculty of Dentistry, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

K.H. Chua

Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

C.K. Low

Laboratory Animal Resource Unit, Faculty of Medicine

Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz

50300 Kuala Lumpur, Malaysia

A.J. Mohd. Nazimi, N. Rafiqah & R. Ramli

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry

Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff, Cheras

56000 Kuala Lumpur, Malaysia

Y. Norziha & A.S. Shariffal Shuriana

Department of Prosthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

C.H. Siar

Department of Oral Pathology, Oral Medicine and Periodontology

Faculty of Dentistry, University of Malaya

50603 Kuala Lumpur, Malaysia

Diserahkan: 17 Februari 2011 / Diterima: 2 November 2011

ABSTRACT

Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction.

Keywords: Bone marrow stem cells; Macaca fascicularis; tissue engineering

ABSTRAK

Pengkulturan sel stem sum-sum tulang Macaca fascicularis di dalam (FBS) fetal bovine serum (FBS) menghasilkan proliferasi sel yang rendah serta tempoh inkubasi yang panjang. Kami melaporkan penambahbaikan teknik pengkulturan sel stem sum-sum tulang Macaca fascicularis ini dengan menggunakan FBS bersama-sama dengan serum Macaca fascicularis. Serum (5%) ditambah kepada Minimum Essential Medium (MEM) alpha medium dan 10% FBS manakala 0.2 mM asid askorbik 2-fosfat, 10 mM β-glyserolfosfat dan 10-8 molar deksamethason digunakan untuk pengaruhan tulang. Kajian ini menunjukkan pertumbuhan kinetik sel yang tinggi iaitu 1.41% lebih tinggi jika dibandingkan dengan 0.46% pengkulturan sel tanpa kehadiran serum Macaca fascicularis manakala masa untuk populasi sel berganda pula mengambil masa di antara 4 hingga 7 hari di dalam kultur yang menggunakan serum berbanding dengan 30 hari kultur tanpa serum. Ini menunjukkan kombinasi serum ini dapat mempercepatkan pertumbuhan sel dan pembentukan

konstruk tisu tulang.

Kata kunci: Kejuruteraan tisu; Macaca fascicularis; sel stem sum-sum tulang

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*Pengarang untuk surat-menyurat; email: roza@medic.ukm.my