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Antioxidant Activity of Ardisia crispa (Mata pelanduk)
(Aktiviti
Antioksida daripada Ardisia crispa (Mata pelanduk))
Hassan Mahmood Kzar Jindal & Jamaludin Mohamad*
Institute of Biological Sciences, Faculty
of Science
University of Malaya, 50603 Kuala Lumpur, Malaysia
Diserahkan: 11 April 2011/Diterima: 11 November 2011
ABSTRACT
The leaf and fruit crude extracts of
hexane, chloroform, methanol and water of Ardisia
crispa were screened for their antioxidant activity using DPPH radical
scavenging, ferric reducing power, and metal chelating antioxidant assay. The
methanol crude extract of fruits showed higher antioxidant activity (90.16 ±
0.01%) than the methanol crude extract of leaves (82.24 ± 0.02%) in the DPPH radical
scavenging assay. In the ferric reducing power assay methanol fruit extract
showed the highest absorbance indicating high antioxidant activities than leaf
extract. In the metal chelating antioxidant assay fruit methanol extract gave
40% antioxidant activities than the leaf. Thin Layer Chromatography of the
fruit methanol crude extract showed that it contained phenolic compounds when
it was detected with folin reagent. HPLC analysis
revealed that the fruit methanol extract contained gallic acid. This indicated
that the high antioxidant activities of the fruits were due to the presence of
gallic acid in the fruits of Ardisia
crispa.
Keywords: Antioxidants; DPPH radical scavenging activity; gallic acid; metal chelating
activity; reducing power assay
ABSTRAK
Ekstrak mentah heksan, kloroform,
metanol, air daripada daun dan buah Ardisia
crispa telah disaring untuk menentukan aktiviti antioksidan
menggunakan asai antioksidan penyahbebas radikal DPPH, kuasa
penurunan ferik, dan logam pengkelat. Ekstrak metanol buah menunjukkan aktiviti
antioksidan yang lebih tinggi daripada ekstrak daun dengan peratus perencatan
penyahbebas DPPH 90.16 ± 0.01% and 82.24
± 0.02% masing-masing. Dalam asai kuasa penurunan ferik
ekstrak metanol buah menunjukkan serapan yang tertinggi menandakan aktiviti
antioksidan yang tinggi daripada ekstrak daun. Dalam
asai logam pengkelat ekstrak metanol, buah memberikan 40% aktiviti antioksidan
daripada daun. Kromatografi lapisan nipis ekstrak
mentah buah menunjukkan, terdapat kandungan sebatioan fenolik. Analisis HPLC menunjukkan akstrak mentah
mengandungi asid galik. Ini menunjukan aktiviti antioksidan yang tinggi
di dalam buah adalah disebabkan kehadiran asid galik dalam buah Ardisia crispa.
Kata
kunci: Aktiviti pengkelat logam; antioksidan; asai kuasa penurun; asid galik;
penyahbebas radikal DPPH
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*Pengarang untuk
surat-menyurat; email: jamal@um.edu.my
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