Sains Malaysiana 43(12)(2014): 1883–1887

Quantitative HPLC Analysis of Gallic Acid in Benincasa hispida Effect of Different

Cations in Pickling Solution on FTIR Characteristics of Pidan White and Yolk in Comparison to the Fresh Duck Egg

(Kesan Kation Berbeza dalam Larutan Jeruk ke atas Ciri FTIR Putih dan Kuning Pidan

Berbanding Telur Itik Segar)

PALANIVEL GANESAN^1,2*, SOOTTAWAT BENJAKUL² & BADLISHAH SHAM BAHARIN¹

¹Department of Food Technology, Faculty of Food Science and Technology

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

²Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University

Hat Yai, Songkhla, 90112, Thailand

Diserahkan: 25 September 2013/Diterima: 2 Mei 2014

ABSTRACT

Fourier transform infrared (FTIR) study of pidan white and yolk treated with different cations were investigated in comparison to the fresh duck egg. FTIR study of PbO₂ and ZnCl₂ treated pidan white and yolk at a level of 2 g kg⁻¹ had different spectra to those of fresh egg. The amide B with wavenumbers of 3083 and 3084 cm^-1 was observed for fresh and PbO₂ treated pidan white at a level of 2 g kg^-1. Higher wavenumber of 3084 cm^-1 was noticeable for PbO₂ treated pidan white at a level of 2 g kg^-1. Scanning electron microscopic study showed that the more ordered network was found in PbO₂ treated pidan white at a level of 2 g kg⁻¹, compared with ZnCl₂ treated counterpart. Thus cations in the pickling solution affected the FTIR characterestics of pidan white and yolk.

Keywords: CLSM; FTIR; pidan; SEM

ABSTRAK

Penyelidikan transformasi Fourier inframerah (FTIR) putih dan kuning pidan yang dirawat dengan kation berbeza berbanding telur itik segar dikaji. Kajian FTIR PbO₂ dan ZnCl₂ putih dan kuning pidan pada tahap 2 g kg^-1 mempunyai bilangan spektra yang berbeza dengan telur segar. Amida B dengan gelombang nombor 3083 dan 3084 cm^-1 diperhatikan pada PbO₂ putih pidan yang dirawat pada tahap 2 g kg^-1. Gelombang nombor yang lebih tinggi daripada 3084^-1 adalah ketara bagi putih pidan dirawat dengan PbO₂  pada tahap 2 g kg^-1. Kajian imbasan elektron microskopi menunjukkan bahawa rangkaian lebih tersusun dilihat pada putih pidan PbO₂ yang dirawat pada tahap 2 g kg⁻¹, berbanding dengan yang dirawat oleh ZnCl₂. Oleh itu kation dalam larutan jeruk memberi kesan kepada ciri FTIR putih dan kuning pidan.

Kata kunci: CLSM; FTIR; pidan; SEM

RUJUKAN

Boye, J.I., Ismail, A.A. & Alli, I. 1996. Effect of physico-chemical factors on the secondary structure of lactoglobulin. Journal of Dairy Research 63: 97-109.

Chang, H.M., Tsai, C.F. & Li, C.F. 1999. Quantification of racemization of amino acids in alkali-treated duck eggs by micellar capillary electrophoresis. Journal of Agricultural and Food Chemistry 47: 479-484.

Chen, J.W. & Su, H.P. 2004. A new process for preparing spots-free pidan. Journal of the Chinese Society of Animal Science 33: 79-88.

Creighton, T.E. 1993. Proteins in solution and in membranes. In: Proteins: Structures and Molecular Properties. 2nd ed., edited by Creighton, T.E. New York: W.H. Freeman & Co.

Ganasen, P. & Benjakul, S. 2011a. Effects of green tea and Chinese tea on the composition and physical properties of pidan white. Journal of Food Processing & Technology 35: 907-916.

Ganasen, P. & Benjakul, S. 2011b. Physical properties and microstructure of pidan white as affected by different divalent and monovalent cations. Journal of Food Biochemistry 35: 1528-1537.

Ganasen, P. & Benjakul, S. 2010. Physical properties and microstructure of pidan yolk as affected by different divalent and monovalent cations. LWT - Food Science and Technology 43: 77-85.

Guillen, M.D., Ruiz, A. & Cabo, N. 2004. Study of oxidative degradation of farmed salmon lipids by means of Fourier transform infrared spectroscopy. Influence of salting. Journal of the Science of Food and Agriculture 84: 1528-1534.

Guillén, M.D. & Cabo, N. 1997. Infrared spectroscopy in the study of edible oils and fats. Journal of the Science of Food and Agriculture 75: 1-11.

Heertje, I. & van Kleef, F.S.M. 1986. Observations on the microstructure and rheology of ovalbumin gels. Food Microstructure 5: 91-98.

Kiosseoglou, V. 2003. Egg yolk protein gels and emulsions. Current Opinion in Colloid and Interface Science 8: 356-70.

Mineki, M. & Kobayashi, M. 1997. Microstructure of yolk from fresh eggs by improved method. Journal of Food Science 62: 757-761.

Nagarajan, M., Benjakul, S., Prodpran, T., Songtipya, P. & Kishimura, H. 2013. Effects of bleaching on characteristics and gelling property of gelatin from splendid squid (Loligo formosana) skin. Food Hydrocolloids 32: 447-452.

Nagarajan, M., Benjakul, S., Prodpran, T., Songtipya, P. & Kishimura, H. 2012. Characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocolloids 29: 389-397.

Setiowaty, G., Che-Man, Y.B., Jinap, S. & Moh, M.H. 2000. Quantitative determination of peroxide value in thermally oxidised palm olein by Fourier transform infrared spectroscopy. Phytochemical Analysis 11: 74-78.

Steel, R.G.D. & Torrie, J.H. 1980. Principle and Procedure of Statistics. 2nd ed. New York: McGraw-Hill.

Van de Voort, F.R., Ismail, A.A., Sedman, J. & Emo, G. 1994. Monitoring the oxidation of edible oils by transform infrared spectroscopy. Journal of the American Chemical Society 71: 243-253.

Woodward, S.A. & Cotterill, O.J. 1987. Texture and microstructure of cooked whole egg and heat-formed gels of stirred egg yolk. Journal of Food Science 52: 63-67.

*Pengarang untuk surat-menyurat; email: palanivel@upm.edu.my