Sains Malaysiana 43(4)(2014): 559–566

Kesan Apigenin, Berberin dan Rutin terhadap Metabolisme Kolesterol pada Sel Kanser Hep G2

(Effect of Apigenin, Berberine and Rutin on Cholesterol Metabolism in Hep G2 Cancer Cell)

MOHD KAMAL NIK HASSAN¹*, RASADAH MAT ALI¹, ZULKHAIRI HJ. AMOM², MOHD SHAHIDAN MOHD ARSHAD¹, ZAMREE MD SHAH¹, KHAIRUL KAMILAH ABDUL KADIR¹

& IHSAN SAFWAN KAMARAZAMAN¹

¹Bahagian Hasilan Semula Jadi, Pusat Penyelidikan Perhutanan Malaysia, 52109 Kepong, Kuala Lumpur

Malaysia

²Fakulti Sains Kesihatan, UiTM Kampus Puncak Alam, 42300 Puncak Alam, Selangor

Malaysia

Diserahkan: 12 Mac 2012/Diterima: 31 Julai 2013

ABSTRAK

Dalam kajian ini, keupayaan Apigenin, Berberin dan Rutin untuk mengurangkan metabolisme kolesterol pada sel kanser hepatoma manusia (Hep G2) telah ditentukan. Penilaian sitotoksik Apigenin, Berberin dan Rutin telah dilakukan dengan mendedahkan Hep G2 kepada Apigenin, Berberin dan Rutin pada kepekatan antara 7.8 sehingga 1000 μg/mL selama 24 jam pada suhu 37^oC dan 5% atmosfera CO₂. Apigenin, Berberin dan Rutin masing-masing mempunyai kepekatan perencat 20 (IC₂₀) 7.8, 125 dan 1000 μg/mL. Keupayaan mengurangkan metabolisme kolesterol oleh Apigenin, Berberin dan Rutin pada Hep G2 telah diuji dengan penyemaian Hep G2 dalam plat 6-telaga. Kumpulan rawatan Apigenin, Berberin dan Rutin telah dirawat dengan kepekatan 7.8, 31.25 dan 62.5 μg/mL masing-masing dan didedah dengan lipoprotein ketumpatan rendah (LDL) sebanyak 10 μL. Dalam kumpulan kawalan normal (NC), Hep G2 telah dieram dengan media kultur sahaja. Sel diinkubasikan dan media telah diambil untuk analisis Apo A1, LCAT, LDLR dan FDFT1 dengan menggunakan kit. Rutin didapati mampu merendahkan aktiviti HMGR secara signifikan (p<0.05) berbanding kawalan normal. Apigenin dan Berberin mampu meningkatkan kepekatan APO A1. Ketiga – tiga sampel mampu meningkatkan kepekatan LCAT pada sel yang dirawat. Selain itu, Apigenin mampu meningkatkan kepekatan LDLR. Keputusan ujian untuk FDFT1 menunjukkan Berberin dan Rutin merendahkan kepekatan FDFT1 dan berbeza secara signifikan (p<0.05) berbanding kawalan normal. Penemuan ini menunjukkan bahawa Apigenin, Berberin dan Rutin mempunyai potensi dalam mengurangkan metabolisme kolesterol dalam sel Hep G2.

Kata kunci: Apigenin; Berberin; metabolisme kolesterol; Rutin

ABSTRACT

In this study, the ability of Apigenin, Berberine and Rutin to reduce cholesterol metabolism in human hepatoma cancer cell line (Hep G2) was determined. Cytotoxic assessment of Apigenin, Berberine and Rutin were performed by exposing the Hep G2 to Apigenin, Berberine and Rutin at concentrations ranging from 7.8 to 1000 μg/mL for 24 h at 37oC and with 5% CO₂ atmosphere. The inhibition concentration 20 (IC₂₀) of Apigenin, Berberine and Rutin were 7.8, 125 and 1000 μg/mL, respectively. The ability of reducing cholesterol metabolism effects of Apigenin, Berberine and Rutin on Hep G2 was carried out by seeding the cell in 6-well plates. In the treated groups, Hep G2 was treated with Apigenin, Berberine and Rutin (7.8, 31.25 and 62.5 μg/mL, respectively) and exposed to 10 μL low density lipoprotein (LDL). In the normal control (NC) groups, Hep G2 was incubated with culture medium only. The cell was incubated and media was taken for analysis of Apo A1, LCAT, LDLR and FDFT1 by using kit. Rutin was able to lower down HMGR activity significantly (p<0.05) compared with normal control. Apigenin and Berberin were able to increase the concentration of APO A1. All the three samples can increase the concentration of LCAT in treated cells. In addition, Apigenin can increase the concentration of LDLR. The test results for FDFT1 showed that Berberin and Rutin can lower down FDFT1 concentrations and significantly different (p<0.05) compared with normal controls. These findings suggested that Apigenin, Berberine and Rutin have the potential in reducing cholesterol metabolism in Hep G2 cancer cell lines.

Keywords: Apigenin; Berberine; cholesterol metabolism; Rutin

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