Sains Malaysiana 44(8)(2015): 1119–1123

Molecular Characterization of Malaysian Marine Fish Species using Partial Sequence of Mitochondrial DNA 12S and 16S rRNA Markers

(Pencirian Molekul Spesies Ikan Laut Malaysia menggunakan Urutan Separa Penanda Mitokondria DNA 12S dan 16S rRNA)

SITI FARAH QURAISHIA, SUNDARARAJULU PANNEERCHELVAM, ZAFARINA ZAINUDDIN

& NUR HASLINDAWATY ABD RASHID*

School of Health Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian,

Kelantan Darul Naim, Malaysia

Diserahkan: 17 November 2014/Diterima: 2 April 2015

ABSTRACT

This study was conducted to characterize the selected marine fish species using partial sequence of mtDNA 12S and 16S rRNA gene. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 350 and 440 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic tree was constructed using MEGA software. Two reference species have been used namely Gobio gobio (AB239596 and EF112528) and Pentapodus caninus (DQ533268 and DQ532933). Based on the result obtained, mtDNA 12S and 16S rRNA were found to be useful as molecular markers for fish species identification. These markers will provide correct identification of fish species when considered along with morphological characteristics.

Keywords: Mitochondrial DNA; species identification; 12S rRNA; 16S rRNA

ABSTRAK

Kajian ini dijalankan untuk mencirikan spesies ikan laut terpilih dengan menggunakan urutan separa gen mtDNA 12S dan 16S rRNA. PCR amplifikasi 12S rRNA dan 16S rRNA masing-masing menghasilkan amplikon PCR pada 350 dan 440 bp panjang. Analisis urutan dilakukan dengan menggunakan perisian Bioedit. Pokok filogeni dihasilkan dengan menggunakan perisian MEGA. Dua spesies rujukan digunakan iaitu Gobio gobio (AB239596 dan EF112528) dan Pentapodus caninus (DQ533268 dan DQ532933). Berdasarkan keputusan yang diperoleh, mtDNA 12S dan 16S rRNA didapati berguna sebagai penanda molekul bagi pengenalpastian spesies ikan. Penanda ini akan menyediakan pengenalan yang betul bagi spesies ikan apabila digunapakai bersama-sama dengan ciri morfologi.

Kata kunci: Mitokondria DNA; pengenalpastian spesies; 12S rRNA; 16S rRNA

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*Pengarang untuk surat-menyurat; email: haslindawaty@usm.my