Sains Malaysiana 45(12)(2016): 1923–1930

http://dx.doi.org/10.17576/jsm-2016-4512-17

 

Kesan Perencatan Madu Gelam terhadap Kehilangan Tulang pada Tikus Periodontitis

(Inhibitory Effect of Gelam Honey on Bone Loss in Periodontitis Rat)

 

AFIFAH-RADIAH FAUZI¹*, SALIANA A. AZIZ², NORASHIKIN HAMZAH², BADIAH BAHARIN², NORZANA ABDUL GHAFAR³ & MARIATI ABDUL RAHMAN¹

 

 

¹Jabatan Biologi Mulut dan Klinikal, Fakulti Pergigian, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

²Jabatan Periodontologi, Fakulti Pergigian, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

³Jabatan Anatomi, Fakulti Perubatan, Pusat Perubatan Universiti Kebangsaan Malaysia

Jalan Yaacob Latif, 56000 Cheras, Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

Diserahkan: 26 April 2016/Diterima: 14 Oktober 2016

 

ABSTRAK

Periodontitis adalah penyakit kronik yang melibatkan kehilangan tulang dan inflamasi pada tisu periodontium. Interleukin-6 (IL-6) dan Tumor Necrosis Factor-α (TNF-α) merupakan penanda pro-inflamasi yang penting yang terlibat dalam periodontitis. Sebanyak 20 ekor tikus Sprague-Dawley dibahagikan kepada empat kumpulan iaitu: Kumpulan kawalan dengan salin normal (CS); kumpulan kawalan dengan madu Gelam 3 g/mL (CH); kumpulan ujian periodontitis dengan salin normal (TS); dan kumpulan ujian periodontitis dengan madu Gelam 3 g/mL (TH). Benang bersaiz 4/0 diikat pada molar pertama gigi tikus sebelah kiri bagi tujuan rangsangan penyakit periodontitis. Madu Gelam diberi secara paksa oral selama 15 hari. Selepas 15 hari, sampel plasma dan tisu dianalisis menggunakan kaedah Elisa dan pewarnaan histologi. Kehilangan tulang alveolar pada kumpulan TS adalah paling tinggi berbanding dengan kumpulan kawalan, CS dan CH namun, tiada perbezaan yang signifikan berbanding dengan kumpulan TH. Berdasarkan ujian imunohistokimia, ekspresi IL-6 dan TNF-α pada tisu periodontium adalah tinggi secara signifikan pada kumpulan TS berbanding dengan kumpulan lain. Namun, tiada perubahan aras IL-6 dan TNF-α yang signifikan pada plasma ke semua tikus kajian.

 

Kata kunci: Kehilangan tulang; madu; perantara inflamasi; periodontitis

 

ABSTRACT

Periodontitis is a chronic disease which results in alveolar bone loss and inflammation at the periodontal gingival tissue. Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) are the crucial pro-inflammatory markers in periodontitis. Twenty Sprague-Dawley rats were divided into four groups: which was control group (CS); control treated with Gelam honey (3 g/mL) (CH); periodontitis group (TS); and periodontitis treated with Gelam honey (3 g/mL) (TH). The periodontitis was induced by ligating 4/0 suture on the left first molar tooth. After 15 days, the plasma and tissue samples were analysed using Elisa and histological staining. Alveolar bone loss was the highest in the TS group compared to control groups, CS and CH, but there was no significant difference compared to TH group. Immunohistochemistry staining showed positive expression of IL-6 and TNF-α in periodontium was significantly higher percentage in TS group compared to TH group which was 49.27% (IL-6) and 31.73% (TNF-α), respectively. However, there was no significant difference in plasma level of IL-6 and TNF-α.

 

Keywords: Bone loss; honey; inflammatory mediators; periodontitis

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*Pengarang untuk surat-menyurat; email: afifahradiah@yahoo.com.my