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Sains Malaysiana 47(11)(2018): 2693–2698 http://dx.doi.org/10.17576/jsm-2018-4711-12
(-)-Glaciantarcin,
a New Dipeptide and Some Secondary Metabolites from the Psychrophilic
Yeast Glaciozyma antarctica
PI12 ((-)-Glasiantarsin,
satu Dipeptida
Terbaharu dan Beberapa
Metabolit Sekunder
daripada Yis Psikrofilik
Glaciozyma antarctica PI12) ANDI R.
ROSANDY1,
YEOH
CHIANN
YING3,
CHEAH
YOKE
KQUEEN3,
SENG
JOE LIM1,
JALIFAH
LATIP1,
A.
MUNIR
A.
MURAD2,
MUNTAZ
A.
BAKAR1
& ROZIDA M. KHALID1* 1School of Chemical Science and Food
Technology, Faculty of Science and Technology, Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia 2School of Biosciences and Biotechnology,
Faculty of Science and Technology, Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia 3Department of Biomedical Sciences,
Faculty of Medicine and Health Sciences, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor
Darul Ehsan, Malaysia Diserahkan: 26 Mac 2018/Diterima:
6 Julai 2018 ABSTRACT A new dipeptide, (-)-glaciantarcin (1) and three known compounds, cyclo(-Pro-Gly) (2), 1-(2-deoxypentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione
(3) and vidarabine (Ara-A) (4),
were isolated from Glaciozyma antarctica PI12, a cold-adapted yeast. The chemical structures
were elucidated by FT-IR, NMR and
mass spectrometry. The cytotoxicity and antioxidant activities
of compounds 1-4 were evaluated by using the MTT bioassay on MCF-7
(human breast cancer cell line), PC-3 (human prostate cancer cell
line) and HEK-293 (normal human embryonic kidney
cell line) and DPPH free radical scavenging activity,
respectively. At concentration of 400 μM,
all compounds showed the highest activity on MCF-7, with compound 1 at
65%, compound 2 (70%), compound 3 (66%) and compound
4 (58%) cell viability. All compounds exhibited weak antioxidant
properties. To the best of our knowledge, this is the first report
of compounds 1-4 from Glaciozyma
antactica. Keywords: Biological activities;
dipeptide; (-)-glaciantarcin; Glaciozyma
antarctica
PI12; psychrophilic
yeast ABSTRAK Satu dipeptida
terbaharu iaitu
(-)-glasiantarsin (1) dan tiga sebatian yang telah diketahui; siklo (-Pro-Gli) (2), 1-
(2-Deoksipentofuranosil) -5-metil-2,4
(1H, 3H) -pirimidinedion (3) dan vidarabin (Ara-A) (4) telah
diasingkan daripada
Glaciozyma antarctica
PI12, yis yang teradaptasi
sejuk. Struktur kimia dijelaskan
oleh spektroskopi
inframerah (IM), ultra lembayung (UL), resonans magnetik nuklear (RMN)
dan spektroskopi
jisim. Aktiviti kesitotoksikan
dan antioksidan
daripada sebatian 1-4 dinilai
menggunakan bioasai
MTT ke atas MCF-7 (sel kanser payudara
manusia), PC-3 (sel
kanser prostat manusia) dan sel
HEK-293 (sel normal)
serta aktiviti perencatan radikal bebas DPPH. Pada kepekatan 400 μM, semua sebatian menunjukkan aktiviti tertinggi ke atas MCF-7,
iaitu masing-masing:
sebatian 1 (62 ± 0.9), 2 (66 ± 1.6), 3 (62
± 1.3) dan 4 (55 ± 1.7). Kesemua sebatian
terpencil menunjukkan
sifat antioksidan yang lemah. Sepanjang pengetahuan kami, ini
merupakan laporan
pertama sebatian 1-4 daripada Glaciozyma antactica.
Kata kunci: Aktiviti biologi; dipeptida; (-)-glasiantarsin; Glaciozyma
antarctica
PI12; yis psikrofilik RUJUKAN
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untuk surat-menyurat;
email: rozidakhalid@ukm.edu.my
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