Sains Malaysiana 48(10)(2019): 2191–2199

http://dx.doi.org/10.17576/jsm-2019-4810-15

The Neuroprotective Effect of Nasturtium officinale on Learning Ability and Density of Parvalbumin Neurons in the Hippocampus of Neurodegenerative-Induced Mice Model

(Kesan Neuropelindung Nasturtium officinale pada Keupayaan Pembelajaran dan Kepadatan Neuron Parvalbumin di Hipokampus Model Tikus Teraruh Neurodegeneratif)

WARIT RUANGLERTBOON¹, EKKASIT KUMARNSIT², SUKANYA DEJ-ADISAI³, URAPORN VONGVATCHARANON⁴ & WANDEE UDOMUKSORN¹*

¹Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand

²Department of Physiology, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand

³Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand

⁴Department of Anatomy, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand

Diserahkan: 14 Februari 2019/Diterima: 20 Ogos 2019

ABSTRACT

According to European traditional pharmacopeia, as well as in Chinese traditional medicine, watercress (Nasturtium officinale) has a property in enhancing physical stamina during stress condition. The aim of the study was to evaluate the neuroprotective effects of watercress extract (WCE) on dexamethasone-induced neurodegeneration in mice. Swiss albino male mice (35-40 g) were divided into 6 groups: double distilled water (DW) and normal saline solution (NSS) (DW+NSS), DW and dexamethasone (DW+DEX)-treated for 21 days, 80 mg/kg of watercress extract (WCE) and NSS (80WCE+NSS), and WCE-treated (14 days prior to and during dexamethasone treatment) at variable doses of 20 mg/kg (20WCE+DEX), 40 mg/kg (40WCE+DEX), and 80 mg/kg (80WCE+DEX). At the end of the experiment, animals were tested for spatial memory and learning ability by Morris Water Maze apparatus to determine the escape latency time (ELT), and the density of parvalbumin (PV)-immunoreactive (PV-ir) neurons in the hippocampus of the brain, using immunohistochemistry. After dexamethasone treatment, the animals had significantly lower body weight, higher ELT and reduced density of PV-ir neurons in the CA1 and CA2 regions of the hippocampus, compared to the control animals. These parameters partially improved in animals supplemented with WCE but without a dose-related pattern. This study suggests that WCE may be beneficial for neuroprotection in stress-induced neurodegeneration.

Keywords: Hippocampus; Nasturtium officinale; neurodegeneration; parvalbumin neurons; spatial memory

ABSTRAK

Menurut farmakopeia tradisi Eropah dan perubatan tradisi China, selada air (Nasturtium officinale) mempunyai sifat dalam meningkatkan stamina fizikal semasa keadaan stres. Tujuan kajian ini adalah untuk menilai kesan neuropelindung daripada ekstrak selada air (WCE) pada deksametason-teraruh neurodegenerasi pada tikus. Tikus jantan Swiss Albino (35-40 g) telah dibahagikan kepada 6 kumpulan: Air suling dua kali (DW) dan larutan garam biasa (NSS) (DW + NSS), DW dan deksametason (DW + DEX)-dirawat selama 21 hari, 80 mg/kg daripada ekstrak selada air (WCE) dan NSS (80WCE + NSS), dan WCE-dirawat (14 hari sebelum dan semasa rawatan deksametason) pada pelbagai dos 20 mg/kg (20WCE + DEX), 40 mg/kg (40WCE + DEX), dan 80 mg/kg (80WCE + DEX). Pada akhir uji kaji, haiwan telah diuji untuk ingatan ruang dan keupayaan belajar menggunakan perkakasan Lorongan Keliru Morris untuk menentukan waktu lepas kependaman (ELT), dan ketumpatan neuron parvalbumin (PV)-imunoreaktif (PV-ir) pada hipokampus otak menggunakan imunohistokimia. Selepas rawatan deksametason, tikus mempunyai berat badan yang jauh lebih rendah, ELT lebih tinggi dan kurang ketumpatan neuron PV-ir di kawasan CA1 dan CA2 hipokampus berbanding dengan tikus kawalan. Parameter ini sebahagiannya bertambah baik pada haiwan yang diberikan WCE tetapi tanpa corak yang berkaitan dengan dos. Kajian ini menunjukkan bahawa WCE mungkin bermanfaat untuk neuropelindung dalam neurodegeneratif teraruh tekanan.

Kata kunci: Hipokampus; ingatan ruang; Nasturtium officinale; neurodegeneratif; parvalbumin neuron

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*Pengarang untuk surat-menyurat; email: wandee.u@psu.ac.th