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Sains Malaysiana 48(2)(2019): 291–299
http://dx.doi.org/10.17576/jsm-2019-4802-04
Survey
of Viral Hemorrhagic Septicemia Virus (VHSV) in Olive Flounder, Paralichthys
olivaceus Hatchery in Korea
(Tinjauan
Virus Berdarah Septisemia Viral (VHSV) di Tempat Penetasan
MIN-SEOK JANG1, MYUNG-JOO OH1, SEOK-RYEL KIM2 & WI-SIK KIM1*
1Department
of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University,Yeosu 59626, Korea
2Southeast Sea
Fisheries Research Institute, National Fisheries Research and Development
Institute, Tongyeong 53085, Korea
Diserahkan: 9 April 2018/Diterima: 15 Oktober 2018
ABSTRACT
Viral hemorrhagic septicemia (VHS)
generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the
hatchery to on-growing system in Korea during spring. However, it remains
unclear whether fish are infected by VHS virus (VHSV)
in the hatchery or the on-growing system. In the present study, a survey was
conducted to investigate VHSV infection in 39 olive flounder
hatcheries from 2014 to 2017. Fish were tested for the presence of VHSV by inoculating sample to fathead minnow (FHM)
and chinook salmon embryo (CHSE-214) cells to observe cytopathic
effect, reverse transcription-polymerase chain reaction (RT-PCR),
and antibody detection enzyme-linked immunosorbent assay (ELISA). VHSV was not detected in any of the 2,430 fish (from 461 pooled and
156 unpooled samples), although 34 (20.3%) of 167 samples was found to be
positive for marine birnavirus (MABV) by cell culture and RT-PCR.
Antibody detection ELISA results showed that all 212 fish
sera have optical density (OD) values below of 0.1, suggesting
that these fish had no VHSV-specific antibodies. Moreover, VHSV was not detected in any of 40 pooled samples (172 fish) collected
after shifting rearing water temperature from 17-21°C to 10-15°C. In
conclusion, the 39 olive flounder hatcheries surveyed in Korea was not infected
by VHSV.
Keywords: Hatchery; olive flounder; survey; viral hemorrhagic septicemia
virus
ABSTRAK
Virus septisemia berdarah (VHS)
biasanya berlaku selepas juvenil olive flounders (Paralichthys
olivaceus) berpindah dari tempat penetasan ke sistem perkembangan
di Korea semasa musim bunga. Walau bagaimanapun, ia masih tidak
jelas sama ada ikan telah dijangkiti oleh virus VHS (VHSV)
di tempat penetasan atau dalam sistem perkembangan. Dalam kajian
ini, suatu tinjauan telah dijalankan untuk mengkaji jangkitan VHSV pada
39 tempat penetasan olive flounder dari 2014 untuk 2017.
Ikan telah diuji untuk kehadiran VHSV melalui inokulasi sampel fathead minnow (FHM)
dan sel embrio chinook salmon (CHSE-214) untuk memerhati kesan
sitopati, tindak balas rantai transkripsi-polimerase berbalik (RT-PCR)
dan pengesanan antibodi enzim berkaitan cerakin imunosorben (ELISA).
VHSV
tidak dikesan dalam mana-mana 2,430 sampel ikan (dari
461 sampel tergembleng dan 156 tak tergembleng), walaupun 34 (20.3%)
167 adalah positif untuk birnavirus laut (MABV) melalui kultur sel dan RT-PCR.
Keputusan pengesanan antibodi ELISA menunjukkan bahawa kesemua
212 sera ikan mempunyai nilai ketumpatan optik (OD)
di bawah 0.1 yang menyarankan bahawa ikan ini tidak mempunyai antibodi
khusus VHSV.
Selain itu, VHSV juga tidak dikesan dalam mana-mana 40 sampel tergembleng
(172 ikan) yang diambil selepas perubahan
suhu air ternakan daripada 17-21°C kepada 10-15°C. Kesimpulannya,
39 tempat penetasan olive flounders yang dikaji di Korea
tidak dijangkiti dengan VHSV.
Kata
kunci: Olive flounder; tempat penetasan; tinjauan; virus septisemia berdarah viral RUJUKAN
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*Pengarang untuk surat-menyurat; email: wisky@jnu.ac.kr
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