Sains Malaysiana 48(3)(2019): 523–531

http://dx.doi.org/10.17576/jsm-2019-4803-04

Structural Properties, Production, and Commercialisation of Invertase

(Sifat Struktur, Pengeluaran dan Pengkomersialan Invertase)

WEI CHENG PANG¹, AIZI NOR MAZILA RAMLI^1,2* & NUR DINI JOHARI¹

¹Faculty of Industrial Science & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia

²Bio Aromatic Research Centre of Excellence, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia

Diserahkan: 30 April 2018/Diterima: 8 Januari 2019

ABSTRACT

The knowledge gained from yeast fermentation made invertase one of the earliest exploited enzymes in human history. Invertase functions as carbohydrases by hydrolysing sucrose into its simplest unit. Extensive studies on invertase have made it well-characterised through the discovery of its existence in a variety of living organisms. It is interesting to study the different types of invertase from either the same or different origins as they might have distinct properties and could possess unique characteristics. With the advancement in technology, the three-dimensional structure, catalytic domain, and mechanism of invertase action have been discovered. Furthermore, it is important to understand how this enzyme has been produced via fermentation or recombinant technology methods. Finally, invertase has been employed in several important industries and its future commercialisation is promising.

Keywords: Commercialisation; invertase; production; structural analysis

ABSTRAK

Pengetahuan yang diperoleh daripada penapaian ragi telah menjadikan invertase sebagai salah satu enzim yang paling awal dieksploitasi di dalam sejarah manusia. Invertase berfungsi sebagai karbohidrase dengan menghidrolisis sukrosa kepada glukosa dan fruktosa. Pelbagai kajian mengenai invertase dan penemuannya dalam pelbagai organisma telah mendalamkan pemahaman tentang invertase. Kajian mengenai invertase daripada punca yang sama atau berlainan adalah menarik kerana ia terdapat ciri-ciri yang berbeza dan unik. Dengan kemajuan bidang teknologi, struktur tiga dimensi, domain pemangkin dan mekanisme tindakan invertase telah dikaji. Tambahan pula, pengetahuan tentang bagaimana enzim ini dihasilkan melalui kaedah penapaian atau kaedah rekombinan juga sangat penting. Akhirnya, invertase telah digunakan dalam pelbagai sektor industri dan pengkomersilannya adalah sangat baik.

Kata kunci: Analisis struktur; invertase; pemasaran; pengeluaran

RUJUKAN

Aburigal, A.A.A., Elkhalifa, E.A., Sulieman, A.M.E. & Elamin, H.B. 2014. Extraction and partial kinetic properties of invertase from Schizosaccharomyces pombe. International Journal of Food Science and Nutrition Engineering 4(3): 80-85.

Addezio, F.D., Yoriyaz, E.J., Maria, C. & Vitolo, M. 2014. Sucrose hydrolysis by invertase using a membrane reactor: Effect of membrane cut-off on enzyme performance. Brazilian Journal of Pharmaceutical Sciences 50(2): 257-259.

Ahmed, K., Valeem, E.E., Mahmood, T., Mahmood, I. & Haq, Q.U. 2015. Optimal cultural conditions for industrial enzyme production by using shaken flask technique of submerged fermentation. FUUAST Journal of Biology 5(1): 21-26.

Aksu, K. & Kutsal, T. 1986. Lactic acid production from molasses utilizing Lactobacillus delbrueckii and invertase together. Biotechnology Letters 8(3): 157-160.

Al-Hagar, O.E.A., Ahmed, A.S. & Hassan, I. 2015. Invertase production by irradiated Aspergillus niger OSH5 using agricultural wastes as carbon source. British Microbiology Research Journal 6(3): 135-146.

Alberto, F., Jordi, E., Henrissat, B. & Czjzek, M. 2006. Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose. Biochemical Journal 395(3): 457-462.

Alberto, F., Bignon, C., Sulzenbacher, G., Henrissat, B. & Czjzek, M. 2004. The three-dimensional structure of invertase (β-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. Journal of Biological Chemistry 279(18): 18903-18910.

Alméciga-Díaz, C.J., Gutierrez, A.M., Bahamon, I., Rodríguez, A., Rodríguez, A.M. & Sánchez, O.F. 2011. Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria. Gene 484(1-2): 26-34.

Altenbach, D. & Ritsema, T. 2007. Structure-function relations and evolution of fructosyltransferases. In Recent Advances in Fructooligosaccharides Research, edited by Shiomi, N., Benkeblia, N. & Onodera, N. Kerala, India: Signpost. pp. 135-156.

Alves, J.N.O., Jorge, J.A. & Guimarães, L.H.S. 2013. Production of invertases by anamorphic (Aspergillus nidulans) and teleomorphic (Emericela nidulans) fungi under submerged fermentation using rye flour as carbon source. Advances in Microbiology 3: 421-429.

Ashraf, H. & Bilal, Z.E.H. 2015. Biosynthesis, partial purification and characterization of invertase through carrot (Daucus carota L.) peels. Journal of Biochemical Technology 6(1): 867-874.

Ayre, G.L. 1967. The relationships between food and digestive enzymes in five species of ants (Hymenoptera: Formicidae). The Canadian Entomologist 99(4): 408-411.

Bagal-Kestwal, D., Kestwal, R.M. & Chiang, B.H. 2015. Invertase-nanogold clusters decorated plant membranes for fluorescence-based sucrose sensor. Journal of Nanobiotechnology 13: 30.

Belcarz, A., Ginalska, G., Lobarzewski, J. & Penel, C. 2002. The novel non-glycosylated invertase from Candida utilis (the properties and the conditions of production and purification). Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology 1594(1): 40-53.

Benkeblia, N., Onodera, S., Yoshihira, T., Kosaka, S. & Shiomi, N. 2004. Effect of temperature on soluble invertase activity, and glucose, fructose and sucrose status of onion bulbs (Allium cepa) in store. International Journal of Food Sciences and Nutrition 55(4): 325-331.

Bergh, M.L., Cepko, C.L., Wolf, D. & Robbins, P.W. 1987. Expression of the Saccharomyces cerevisiae glycoprotein invertase in mouse fibroblasts: Glycosylation, secretion, and enzymatic activity. In Proceedings of the National Academy of Sciences of the United States of America 84: 3570-3574.

Bhatti, H.N., Asgher, M., Abbas, A., Nawaz, R. & Sheikh, M.A. 2006. Studies on kinetics and thermostability of a novel acid invertase from Fusarium solani. Journal of Agricultural and Food Chemistry 54(13): 4617-4623.

Bujacz, A., Jedrzejczak-Krzepkowska, M., Bielecki, S., Redzynia, I. & Bujacz, G. 2011. Crystal structures of the apo form of β-fructofuranosidase from Bifidobacterium longum and its complex with fructose. FEBS Journal 278(10): 1728-1744.

Carlos Martínez, d.R. 1990. Dietary, phylogenetic, and ecological correlates of intestinal sucrase and maltase activity in birds. Physiological Zoology 63(5): 987-1011.

Carnie, J.A. & Porteous, J.W. 1962. The invertase activity of rabbit small intestine. Biochemical Journal 85: 450-456.

Chandra, A., Jain, R. & Solomon, S. 2012. Complexities of invertases controlling sucrose accumulation and retention in sugarcane. Current Science 102(6): 857-866.

Chaudhary, S., Sagar, S., Kumar, M., Sengar, R.S. & Tomar, A. 2015. The use of enzymes in food processing: A review. South Asian Journal of Food Technology and Environment 1(3&4): 190-210.

Chen, T.H., Huang, Y.C., Yang, C.S., Yang, C.C., Wang, A.Y. & Sung, H.Y. 2009. Insights into the catalytic properties of bamboo vacuolar invertase through mutational analysis of active site residues. Phytochemistry 70(1): 25-31.

Deryabin, A.N., Berdichevets, I.N., Burakhanova, E.A. & Trunova, T.I. 2014. Characteristics of extracellular invertase of Saccharomyces cerevisiae in heterologous expression of the Suc2 gene in Solanum tuberosum plants. Biology Bulletin 41(1): 24-30.

Desai, P.N., Shrivastava, N. & Padh, H. 2010. Production of heterologous proteins in plants: Strategies for optimal expression. Biotechnology Advances 28(4): 427-435.

Dominguez, A.L., Rodrigues, L.R., Lima, N.M. & Teixeira, J.A. 2013. An overview of the recent developments on fructooligosaccharide production and applications. Food and Bioprocess Technology 6(12): 1-14.

Dorn, M.E., Silva, M.B., Buriol, L.S. & Lamb, L.C. 2014. Three-dimensional protein structure prediction: Methods and computational strategies. Computational Biology and Chemistry 53: 251-276.

Esawy, M.A., Kansoh, A.L., Kheiralla, Z.H., Ahmed, H.E., Kahil, T.A.K. & El-Hameed, E.K. 2014. Production and immobilization of halophilic invertase produced from honey isolate Aspergillus niger EM77 (KF774181). International Journal of Biotechnology for Wellness Industries 3: 36-45.

Fotopoulos, V. 2005. Plant invertases: Structure, function and regulation of a diverse enzyme family. Journal of Biological Research 4: 127-137.

Galant, A.L., Kaufman, R.C. & Wilson, J.D. 2015. Glucose: Detection and analysis. Food Chemistry 188: 149-160.

Gomes, A.R., Byregowda, S.M., Veeregowda, B.M. & Balamurugan, V. 2016. An overview of heterologous expression host systems for the production of recombinant proteins. Advances in Animal and Veterinary Sciences 4(7): 346-356.

Guimarães, L.H.S. 2012. Carbohydrates from biomass: Sources and transformation by microbial enzymes. In Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology, edited by Chang, C.F. Belgium: Intech. pp. 441-458.

Gurung, N., Ray, S., Bose, S. & Rai, V. 2013. A broader view: Microbial enzymes and their relevance in industries, medicine, and beyond. BioMed Research International 2013: 329121.

Heil, M., Büchler, R. & Boland, W. 2005. Quantification of invertase activity in ants under field conditions. Journal of Chemical Ecology 31(2): 431-437.

Hsiao, C.C., Fu, R.H. & Sung, H.Y. 2002. A novel bound form of plant invertase in rice suspension cells. Botanical Bulletin of Academia Sinica 43: 115-122.

Hsieh, C.W., Liu, L.K., Yeh, S.H., Chen, C.F., Lin, H.I., Sung, H.Y. & Wang, A.Y. 2006. Molecular cloning and functional identification of invertase isozymes from green bamboo Bambusa oldhamii. Journal of Agricultural and Food Chemistry 54: 3101-3107.

Khandekar, D.C., Palai, T., Agarwal, A. & Bhattacharya, P.K. 2014. Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition. Bioprocess and Biosystems Engineering 37(12): 2529-2537.

Kotwal, S.M. & Shankar, V. 2009. Immobilized invertase. Biotechnology Advances 27(4): 311-322.

Kulshrestha, S., Tyagi, P., Sindhi, V. & Yadavilli, K.S. 2013. Invertase and its applications - a brief review. Journal of Pharmacy Research 7(9): 792-797.

Kumar, R. & Kesavapillai, B. 2012. Stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation. SpringerPlus 1: 81.

Kurakake, M., Masumoto, R., Maguma, R., Kamata, A., Saito, E., Ukita, N. & Komaki, T. 2010. Production of fructooligosaccharides by β-fructofuranosidases from Aspergillus oryzae KB. Journal of Agricultural and Food Chemistry 58(1): 488-492.

Lammens, W., Le Roy, K., Schroeven, L., Van Laere, A., Rabijns, A. & Van den Ende, W. 2009. Structural insights into glycoside hydrolase family 32 and 68 enzymes: Functional implications. Journal of Experimental Botany 60(3): 727-740.

Lammens, W., Le Roy, K., Van Laere, A., Rabijns, A. & Van den Ende, W. 2008. Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose. Journal of Molecular Biology 377(2): 378-385.

Li, S., Yang, X., Yang, S., Zhu, M. & Wang, X. 2012. Technology prospecting on enzymes: Application, marketing and engineering. Computational and Structural Biotechnology Journal 2(3): e201209017.

Lincoln, L. & More, S.S. 2017. Bacterial invertases: Occurrence, production, biochemical characterization, and significance of transfructosylation. Journal of Basic Microbiology 57(10): 803-813.

Liu, C., Xu, Z., Cai, S. & Xiong, Z. 2015. CDna cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis. Biologia (Poland) 70(8): 1063-1069.

Madhanasundareswari, K. & Jeyachitra, K. 2015. Production and optimization of growth conditions for invertase enzyme by Aspergillus in solid state fermentation (SSF) using carrot peel as substrate. SIRJ-APBBP 2(1): 16-22.

Maiorano, A.E., Piccoli, R.M., da Silva, E.S. & de Andrade Rodrigues, M.F. 2008. Microbial production of fructosyltransferases for synthesis of pre-biotics. Biotechnology Letters 30(11): 1867-1877.

Marepally, L. 2017. Purification and characterization of invertase from the midgut of fifth instar larvae of Anthereae mylitta Drury (Daba TV). International Journal of Recent Scientific Research 8(6): 17330-17334.

Mehta, K. & Duhan, J.S. 2014. Production of invertase from Aspergillus niger using fruit peel waste as a substrate. International Journal of Pharma and Bio Sciences 5(2): 353-360.

Michel, M.R., Rodríguez-Jasso, R.M., Aguilar, C.N., Gonzalez- Herrera, S.M., Flores- Gallegos, A.C. & Rodríguez-Herrera, R. 2016. Fructosyltransferase sources, production, and applications for prebiotics. In Production Probiotics and Prebiotics in Human Nutrition and Health, edited by Rao, V. Belgium: Intech. pp. 169-189.

Mohandesi, N., Siadat, S.O.R., Haghbeen, K. & Hesampour, A. 2016. Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. 3 Biotech 6: 128-138.

Nadeem, H., Rashid, M.H., Siddique, M.H., Azeem, F., Muzammil, S., Javed, M.R., Ali, M.A., Rasul, I. & Riaz, M. 2015. Microbial invertases: A review on kinetics, thermodynamics, physiochemical properties. Process Biochemistry 50(8): 1202-1210.

Nisha, S., Karthick, A. & Gobi, N. 2012. A review on methods, application and properties of immobilized enzyme. Chemical Science Review and Letters 1(3): 148-155.

Niu, J.Q., Wang, A.Q., Huang, J.L., Yang, L.T. & Li, Y.R. 2014. Isolation, characterization and promoter analysis of cell wall invertase gene SoCIN1 from sugarcane (Saccharum Spp.). Sugar Tech 17(1): 65-76.

Ohara, A., de Castro, R.J.S., Nishide, T.G., Dias, F.F.G., Bagagli, M.P. & Sato, H.H. 2015. Invertase production by Aspergillus niger under solid state fermentation: Focus on physical-chemical parameters, synergistic and antagonistic effects using agro-industrial wastes. Biocatalysis and Agricultural Biotechnology 4(4): 645-652.

Palomares, L.A., Estrada-Mondaca, S. & Ramírez, O.T. 2004. Production of recombinant proteins: Challenges and solutions. In Recombinant Gene Expression, edited by Balbás, P. & Lorence, A. Methods in Molecular Biology 267: 15-52.

Qureshi, A.S., Khushk, I., Ali, C.H., Majeed, H. & Ahmad, A. 2017. Production of invertase from Saccharomyces cerevisiae Angel using date syrup as a cost effective carbon source. African Journal of Biotechnology 16(15): 777-781.

Raju, A.I.C.H., Pulipati, K. & Jetti, A. 2016. Production of invertase by Aspergillus niger under solid state fermentation using orange fruit peel as substrate. Advances in Crop Science and Technology 4: 247.

Rashad, M.M. & Nooman, M.U. 2009. Production, purification and characterization of extracellular invertase from Saccharomyses cerevisiae NRRL Y-12632 by solid-state fermentation of red carrot residue. Australian Journal of Basic and Applied Sciences 3(3): 1910-1919.

Ravindran, R. & Jaiswal, A. 2016. Microbial enzyme production using lignocellulosic food industry wastes as feedstock: A review. Bioengineering 3(4): 30.

Renge, V.C., Khedkar, S.V. & Nandurkar, N.R. 2012. Enzyme synthesis by fermentation method: A review. Scientific Reviews and Chemical Communications 2(4): 585-590.

Ricks, B.L. & Vinson, S.B. 1972. Digestive enzymes of the imported fire ant, Solenopsis richteri (Hymenoptera: Formicidae). Entomologia Experimentalis et Applicata 15: 329-334.

Sainz-Polo, M.A., Ramírez-Escudero, M., Lafraya, A., González, B., Marín-Navarro, J., Polaina, J. & Sanz-Aparicio, J. 2013. Three-dimensional structure of Saccharomyces invertase: Role of a non-catalytic domain in oligomerization and substrate specificity. Journal of Biological Chemistry 288(14): 9755-9766.

Schroeven, L., Lammens, L., Van Laere, A. & Van den Ende, W. 2008. Transforming wheat vacuolar invertase into a high affinity sucrose: Sucrose 1-fructosyltransferase. New Phytologist 180(4): 822-831.

Shah, H.S., Patel, C.M. & Parikh, S.C. 2016. Production of invertase from bacteria by using waste jaggery. The Microbes 3: 19-23.

Shankar, T., Thangamathi, P., Rama, R. & Sivakumar, T. 2014a. Characterization of invertase from Saccharomyces cerevisiae MK obtained from toddy sample. Journal of Bioprocessing and Chemical Engineering 2(1): 1-6.

Shankar, T., Thangamathi, P., Rama, R. & Sivakumar, T. 2014b. Characterization of invertase from Saccharomyces crevisiae MTCC 170. African Journal of Microbiology Research 8(13): 1385-1393.

Shinde, V., Deshmukh, S. & Bhoyar, M.G. 2015. Applications of major enzymes in food industry. Indian Farmer 2(6): 497-502.

Sundarram, A. & Murthy, T.P.K. 2014. α -Amylase production and applications: A review. Journal of Applied & Environmental Microbiology 2(4): 166-175.

Takegawa, K., Tohda, H., Sasaki, M., Idiris, A., Ohashi, T., Mukaiyama, H., Giga-Hama, Y. & Kumagai, H. 2009. Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system. Biotechnology and Applied Biochemistry 53(4): 227-235.

Tauzin, A.S. & Giardina, T. 2014. Sucrose and invertases, a part of the plant defense response to the biotic stresses. Frontiers in Plant Science 5: 1-8.

Ueno, T., Ozawa, Y., Ishikawa, M., Nakanishi, K. & Kimura, T. 2003. Lactic acid production using two food processing wastes, canned pineapple syrup and grape invertase, as substrate and enzyme. Biotechnology Letters 25(7): 573-577.

Uma, C., Gomathi, D., Ravikumar, G., Kalaiselvi, M. & Palaniswamy, M. 2012. Production and properties of invertase from a Cladosporium cladosporioides in SmF using pomegranate peel waste as substrate. Asian Pacific Journal of Tropical Biomedicine 2: 605-611.

Uma, C., Gomathi, D. & Gopalakrishnan, V.K. 2010. Fungal invertase as aid for production of ethanol from sugarcane bagasse. Research Journal of Microbiology 5(10): 980-985.

Voegele, R.T., Wirsel, S., Möll, U., Lechner, M. & Mendgen, K. 2006. Cloning and characterization of a novel invertase from the obligate biotroph Uromyces fabae and analysis of expression patterns of host and pathogen invertases in the course of infection. Molecular Plant-Microbe Interactions 19(6): 625-634.

Van Wyk, N., Trollope, K.M., Steenkamp, E.T., Wingfield, B.D. & Volschenk, H. 2013. Identification of the gene for β-fructofuranosidase from Ceratocystis moniliformis CMW 10134 and characterization of the enzyme expressed in Saccharomyces cerevisiae. BMC Biotechnology 13: 100.

Xie, J., Cai, K., Hu, H.X., Jiang, Y.L., Yang, F., Hu, P.F., Cao, D.D., Li, W.F., Chen, Y. & Zhou, C.Z. 2016. Structural analysis of the catalytic mechanism and substrate specificity of Anabaena alkaline invertase InvA reveals a novel glucosidase. Journal of Biological Chemistry 291(49): 25667-25677.

Yao, Y., Wu, X.H., Geng, M.T., Li, R.M., Liu, J., Hu, X.W. & Guo, J.C. 2014. Cloning, 3D modeling and expression analysis of three vacuolar invertase genes from cassava (Manihot esculenta Crantz). Molecules 19(5): 6228-6245.

Yesilirmak, F. & Sayers, Z. 2009. Heterelogous expression of plant genes. International Journal of Plant Genomics 2009: 296482.

Zárate, V. & Belda, F. 1996. Characterization of the heterologous invertase produced by Schizosaccharomyces pombe from the SUC2 gene of Saccharomyces cerevisiae. The Journal of Applied Bacteriology 80(1): 45-52.

Zhang, J., Scrivener, A.M., Slaytor, M. & Rose, H.A. 1993. Diet and carbohydrase activities in three cockroaches, Calolampra elegans Roth and Princis, Geoscapheus dilatatus Saussure and Panesthia cribrata Saussure. Comparative Biochemistry and Physiology - Part A: Physiology 104(1): 155-161.

Zouaoui, B., Ghalem, B.R., Djillali, B. & Fatima, S. 2016. Characterization of partially purified extracellular thermostable invertase by Streptococcus Sp. isolated from the date. Bulletin of Environment, Pharmacology and Life Sciences 5(9): 65-72.

*Pengarang untuk surat-menyurat; email: aizinor@ump.edu.my