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Sains Malaysiana 48(3)(2019): 629–635
http://dx.doi.org/10.17576/jsm-2019-4803-16
In
vivo Analgesic, Antipyretic and Anti-inflammatory Activities of
Ethanol Extract of Pericampylus glaucus in Experimental Animals
(Aktiviti
Analgesik, Antipiretik dan Anti-Radang secara in vivo Ekstrak Etanol
daripada Pericampylus glaucus pada Haiwan Uji Kaji)
MUHAMMAD KIFAYATULLAH*, HAROON RAHIM, NAEEM ULLAH JAN, KAMRAN AHMAD CHISHTI, IRFAN ULLAH
& SUDAIR ABBAS
Department of Pharmacy, Faculty of Life Sciences, Sarhad
University of Science and Information Technology, Peshawar, Pakistan
Diserahkan: 7 Julai 2018/Diterima: 29 November 2018
ABSTRACT
Pericampylus glaucus is a common Malaysian plant used
traditionally in the treatments of joint pain, abdominal pain and headache.
Hence, the present research was aimed to evaluate ethanolic extract of Pericampylus
glaucus for analgesic, antipyretic and anti-inflammatory activities in
experimental animals. The central and peripheral analgesic activity was
determined by acetic acid induced writhing and hot plate method by examining
the number of writhing and paw licking or jumping time. Meanwhile, the
antipyretic activity was determined by Brewer’s yeast- test that induced
pyrexia and carrageenan- that induced hind paw inflammation was used for
anti-inflammatory activities. The ethanolic extract of Pericampylus glaucus at doses (300 and 600 mg/kg b.wt) and Ibuprofen (100 mg/kg (b.wt) was used
as a reference drug in the whole experiment. Intraperitoneal administration of Pericampylus
glaucus produced significant (p<0.01) inhibition in writhing
response in acetic acid induced writhing test and dose-dependent (p<0.001)
prolonged paw licking in hot plate test as compared to control (normal saline
treated group). Similarly, significant (p<0.001) attenuation in
lowering rectal temperature was noted in animal groups that were treated with
ethanolic extract of Pericampylus glaucus at different doses. The
attenuation was, almost the same as produced by ibuprofen treated group.
Furthermore, Pericampylus glaucus extract also produced significant
reduction in hind paw edema (p<0.001), 4 h after administration of
carrageenan and inhibition was 60.19% and 42.17% as compared to control (normal
saline treated group). The findings of this study indicated that Pericampylus
glaucus possess significant analgesic, antipyretic and anti-inflammatory
activities and could possibly be used in the management of fever, pain and
inflammation.
Keywords: Analgesic; anti-inflammatory; antipyretic; ethanolic
extract; Pericampylus glaucus
ABSTRAK
Pericampylus glaucus adalah tumbuhan yang biasa digunakan
secara tradisi dalam rawatan sakit sendi, sakit badan dan sakit kepala. Oleh
yang demikian, penyelidikan ini bertujuan untuk menilai ekstrak etanol Pericampylus
glaucus untuk aktiviti analgesik, antipiretik dan anti-radang pada haiwan
uji kaji. Aktiviti analgesik pusat dan periferal ditentukan oleh asid asetik
bergelut teraruh dan kaedah plat panas dengan memeriksa kadar bergelut dan
menjilat tapak kaki atau masa lompatan. Manakala aktiviti antipiretik
ditentukan melalui ujian yis Brewer yang mengaruh pireksia dan karaginan - yang
mengaruh inflamasi tapak kaki belakang digunakan untuk aktiviti antiradang.
Ekstrak etanol Pericampylus glaucus pada dos (300 dan 600 mg/kg b.wt)
dan ibuprofen (100 mg/kg b.wt) telah digunakan sebagai dadah rujukan pada uji
kaji keseluruhan. Pemberian intraperitoneum Pericampylus glaucus menghasilkan
perencatan tindak balas bergelut yang signifikan (p<0.01) dalam ujian
bergelut asid asetik dan berkeperluan dos (p<0.001) menjilat tapak
kaki berpanjangan dalam ujian plat panas berbanding kawalan (kumpulan terawat
salin normal). Begitu juga, signifikan (p<0.001) pelemahan hidrologi
merendahkan suhu rektum telah dicatat dalam kelompok haiwan yang telah dirawat
dengan ekstrak ethanolic Pericampylus glaucus pada dos yang berbeza.
Pengecilan ini hampir sama seperti yang dihasilkan oleh kelompok yang dirawat
menggunakan Ibuprofen. Selain itu, ekstrak Pericampylus glaucus juga
menghasilkan pengurangan ketara dalam edema di simpul telapak belakang (p<0.001),
4 jam selepas pemberian carrageenan dan kesan 60.19 dan 42.17% berbanding
kawalan (kelompok salina normal terawat). Keputusan kajian ini menunjukkan
bahawa Pericampylus glaucus mempunyai nilai analgesik, antipiretik dan
anti-radang yang penting dan mungkin boleh digunakan untuk merawat demam, sakit
dan inflamasi.
Kata kunci: Analgesik; anti-radang; antipiretik;
ekstrak etanol; Pericampylus glaucus
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*Pengarang
untuk surat-menyurat; email: kifayatpharma86@yahoo.com
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