Sains Malaysiana 49(1)(2020): 49-55

http://dx.doi.org/10.17576/jsm-2020-4901-06

Simple Extraction of Bisphenol A in Beverages and Water by Membrane-Protected Liquid Phase Microextraction

(Pengekstrakan Mudah Bisfenol A dalam Minuman dan Air menggunakan Pengekstrakan Mikro Fasa Cecair Dilindungi Membran)

THUNG YING LOH, WAN MOHD AFIQ WAN MOHD KHALIK & SAW HONG LOH*

Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

Diserahkan: 24 Jun 2019/Diterima: 2 Oktober 2019

ABSTRACT

Bisphenol A (BPA) is a xenoestrogen that widely used in manufacture of plastics. Concern is mounting up when the application of BPA is widespread. A simple sample pre-treatment technique termed membrane-protected liquid phase microextraction (MP-LPME) combined with high performance liquid chromatography (HPLC)-fluorescence detection (FD) was developed for the determination of BPA in beverages and environmental water samples. The MP-LPME technique utilized a nylon membrane to hold the extractant (1-octanol) and prevent the extractant from dissolution into sample solution during agitation. Under optimal extraction conditions, the MP-LPME-HPLC-FD demonstrated ultra-trace detection of BPA and insignificant matrix effect extraction with good relative recoveries in the range of 87.1-99.7% from spiked beverage and environmental water samples. The membrane can be re-applied in the MP-LPME after the first usage and offered relative recovery of > 94%. The MP-LPME technique is eco-friendly as it consumes only minute amounts of organic solvent which is supportive to green analysis.

Keywords: Bisphenol A; HPLC; liquid phase microextraction; nylon membrane

ABSTRAK

Bisfenol A (BPA) ialah xenoestrogen yang digunakan secara meluas dalam pembuatan plastik. Kebimbangan semakin meningkat apabila aplikasi BPA tersebar luas. Satu teknik pra-rawatan sampel yang mudahdikenali sebagaipengekstrakan mikro fasa cecair yang dilindungi membran (MP-LPME) berpasangan kromatografi cecair prestasi tinggi-pengesanan pendarfluor (HPLC-FD) telah dibangunkan untuk penentuan BPA dalam sampel minuman and air alam sekitar. Teknik MP-LPME menggunakan membran nilon untuk memegang ekstrak (1-oktanol) dan mencegah ekstrak daripada pemelarutan ke dalam larutan sampel semasa pengacauan. Di bawah keadaan pengekstrakan optimum, MP-LPME-HPLC-FD menunjukkan pengesanan ultra surih dan pengekstrakan kesan matriks yang tidak signifikan dengan purata pemulihan semula relatif yang baik dalam linkungan 87.1-99.7% daripada sampel minuman dan air alam sekitar. Membran ini boleh digunakan semula dalam MP-LPME selepas penggunaan pertama dan menawarkan pemulihan semula relatif > 94%. Teknik MP-LPME adalah mesra alam kerana ia hanya menggunakan sedikit pelarut organik dan menyokong analisis hijau.

Kata kunci: Bisfenol A; HPLC; membran nilon; pengekstrakan mikro fasa cecair

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*Pengarang untuk surat-menyurat; email: lohsh@umt.edu.my