Sains Malaysiana 49(6)(2020): 1273-1282

http://dx.doi.org/10.17576/jsm-2020-4906-05

Ameliorative Effect of Apple Pomace and Mango Peels against Hyperlipidemia and Lipid Peroxidation Induced by Hyperlipidemic Diet

 (Kesan Amelioratif Buah Epal dan Kulit Mangga terhadap Hiperlipidemia dan Peroksidasi Lipid disebabkan oleh Diet Hiperlipidemik)

HUMA UMBREEN¹*, MUHAMMAD UMAIR ARSHAD¹, RAZIA NOREEN² & KIRAN AFTAB³

¹Institute of Home and Food Sciences, GC University, Faisalabad, Pakistan

²Department of Biochemistry, GC University, Faisalabad, Pakistan

³Department of Chemistry, GC University, Faisalabad, Pakistan

Diserahkan: 18 Oktober 2018/ Diterima: 17 Februari 2020

ABSTRACT

Diet induced hyperlipidemia is a major cause for atherosclerosis, lipid peroxidation, and fatty liver diseases. Fruit processing waste rich in dietary fiber and antioxidant capacity can be an economical way to manage such risk factors. The major objective of the present study was to manage hyperlipidemia by including apple pomace and mango peels in daily diet. Dry powder obtained from apple pomace, mango peels and their mixture was added to the normal chow on replacement basis by 12%. These were fed to the rats along with hyperlipidemic diet to the experimental groups (N=30, 10/group), while control group (N=10) was fed hyperlipidemic diet alone. The rats were analyzed for water and feed intake, body weight, organs to body weight ratio, glucose concentration, lipid profile, lipid peroxidation test, liver cholesterol, and liver and renal function tests. The results showed that high cholesterol in diet was well managed by rat groups on apple pomace and mango peels supplement. The mango peels powder was found to be more effective as compared to apple pomace powder against hyperlipidemia. Mango peels and apple pomace powders not only improved the lipid profile but also resulted in lower liver cholesterol concentration and better lipid peroxidation status of the experimental rats as compared to control group. It can be concluded that fruit processing waste specially mango peels can be cost effective tool to manage diet induced hyperlipidemia, lipid peroxidation and fatty liver diseases.

Keywords: Antioxidants; fiber; fruit waste; lipid peroxidation; liver cholesterol

ABSTRAK

Diet teraruh hiperlipidemia adalah penyebab utama aterosklerosis, peroksidasi lipid dan penyakit hati berlemak. Serat pemakanan yang kaya dengan sisa pemprosesan buah dan keupayaan antioksidan boleh dijadikan sebagai satu cara yang ekonomi untuk menguruskan faktor risiko tersebut. Objektif utama kajian ini adalah untuk menguruskan hiperlipidemia dengan memasukkan buah epal dan kulit manga dalam diet harian. Serbuk kering yang diperoleh daripada buah epal, kulit manga dan campuran tersebut telah ditambahkan ke dalam chow biasa secara penggantian sebanyak 12%. Makanan diberikan kepada tikus dengan diet hiperlipidemik bagi kumpulan uji kaji (N=30, 10/kumpulan), manakala kumpulan kawalan (N=10) diberi makanan diet hiperlipedemik sahaja. Tikus dianalisis untuk pengambilan air dan makanan, berat badan, nisbah berat organ kepada badan, kepekatan glukosa, profil lipid, ujian peroksidasi lipid, kolesterol hati dan ujian fungsi hati dan ginjal. Hasil kajian menunjukkan bahawa kolesterol tinggi dalam makanan dapat dikendalikan dengan baik oleh kumpulan tikus dengan bahan tambahan buah epal dan kulit mangga. Serbuk kulit mangga didapati lebih berkesan jika dibandingkan dengan serbuk buah epal terhadap hiperlipidemia. Serbuk kulit mangga dan buah epal bukan sahaja meningkatkan profil lipid tetapi juga menghasilkan kepekatan kolesterol hati yang lebih rendah dan status peroksidasi lipid yang lebih baik bagi tikus eksperimen berbanding dengan kumpulan kawalan. Ini dapat disimpulkan bahawa sisa pemprosesan buah khususnya kulit mangga dapat menjadi alat yang efektif daripada segi kos untuk menguruskan hiperlipidemia yang disebabkan oleh diet, peroksidasi lipid dan penyakit hati berlemak.  

Kata kunci: Antioksida; kolesterol hati; peroksidasi lipid; serat; sisa buah

RUJUKAN

Abd El-Ghany, M.A., Ramdan, M.A. & Hassanain, M.S.S. 2011. Antioxidant activity of some agro-industrial peels on liver and kidney of rats exposed to oxidative stress. World Journal of Dairy and Food Science 6: 105-114.

Abeywardena, M.Y. 2003. Dietary fats, carbohydrates and vascular disease: Sri Lankan perspectives. Atherosclerosis 171(2): 157-161.

Afify, A.M.M.R., Romeilah, R.R.M., Osfor, M.M.H. & Elbahnasawy, A.S.M. 2013. Evaluation of carrot pomace (Daucus carota L.) as hypocholesterolemic and hypolipidemic agent on albino rats. Notulae Scientia Biologicae 5(1): 7-14.

Allen, P.J. 2012. Creatine metabolism and psychiatric disorders: Does creatine supplementation have therapeutic values? Neuroscience Biobehaviour Review 36(5): 1442-1462.

Amer, N., Al-Hilfy, J.H.Y. & AL-Taie, M.A.K. 2012. Effect of apple-lite contained of apple fibers and apple gel pectin on body weight, lipid profiles, kidney function and histological structure of kidney in male albino rats. Diyala Journal of Pure Sciences 8(2): 178-187.

Annoni, G., Botasso, B.M., Ciac, D., Donato, M.F. & Tripodi A. 1982. Liquid triglycerides (GPO-PAP). Journal of Laboratory and Clinical Medicine 9: 115.

Anwar, H., Rahman, Z., Sohail, M.U., Iftikhar, A., Hussain, G., IrfanUllah, M., Ali, M.A. & Shaukat, A. 2015. Evaluation of health biomarkers in hyperlipidemic albino rabbits after treatment with Withania somnifera and Lactuca scariola. Pakistan Journal of Life and Social Science 13(1): 31‐36.

Assmann, G. 1979. HDL-cholesterol precipitant. Randox Labs Manual-HDL Cholesterol. Ireland.

Basu, M., Prasad, R., Jayamurthy, P., Pal, K., Arumughan, C. & Sawhney, R.C. 2007. Anti-atherogenic effects of seabuckthorn (Hippophaeal rhamnoides) seed oil. Phytomedicine 14: 770-777.

Bell, E.A. & Rolls, B.J. 2001. Energy density of foods affects energy intake across multiple levels of fat content in lean and obese women. The American Journal of Clinical Nutrition 73(6): 1010-1018.

Chen, G., Wang, H., Zhang, X. & Yang, S. 2014. Nutraceuticals and functional foods in the management of hyperlipidemia. Critical Reviews in Food Science and Nutrition54(9): 1180-1201.

Chen, L., Liu, L., Li, C., Hu, L.C., Su, F., Liu, R., Zeng, M., Zhao, D., Liu, J., Guo, Y. & Long, J. 2017. A mix of apple pomace polysaccharide improves mitochondrial function and reduces oxidative stress in the liver of high-fat diet-induced obese mice. Molecular Nutrition in Food Research 61(3): 1600433.

Cho, K.D., Han, C. & Lee, B. 2013. Loss of body weight and fat and improved lipid profiles in obese rats fed apple pomace or apple juice concentrate. Journal of Medicinal Foods 6(9): 823-830.

Conn, P.M. 2008. Source Book of Models for Biomedical Research. New Jersey: Humana Press.

Durrington, P. & Soran, H. 2014.  Metabolism of Human Diseases. Switzerland: Springer.

Etim, E.I., Essien, E.E., Eseyin, O.A. & Udoh, I.E. 2013. Effect of some artemisinin and combination therapy regimens with and without concomitant administration of phospholipids on the levels of plasma aminotransferases and bilirubin in Nigerian male subjects. African Journal of Pharmacology and Therapeutics 2(1): 17-25.

He, M., van Dam, R.M., Rimm, E., Hu, F.B. & Qi, L. 2010. Whole grain, cereal fiber, bran and germ intake and the risk of all cause and cardiovascular disease-specific mortality among women with type 2 diabetes mellitus. Circulation 121(20): 2162-2168.

Hong, M.Y., Nicole, H., Katy, K., Shirin, H., Arturo, F. & Mark, K. 2015. Watermelon consumption improves inflammation and antioxidant capacity in rats fed an atherogenic diet. Nutrition Research 35(3): 251-258.

Ismail, R.S.A. & Abd, El-Gawad. 2010. Potential effect of Egyptian Anna apple pomace (Malus domestica, Rosaceae) supplementation on kidney function, liver function and lipid profile of diabetic rats. World Journal of Dairy and Food Science 5: 58-66.

Jahurul, M.H.A., Zaidul, I.S.M., Ghafoor, K., Al-Juhaimi, F.Y., Nyam, K., Norulaini, N.A.N., Sahena, F. & Omar, A.K.M. 2015. Mango (Mangifera indica L.) by-products and their valuable components: A review. Food Chemistry 183: 173-180.

Jenkins, D.J., Popovich, D.G., Kendall, C.W., Vidgen, E., Tariq, N., Ransom, T.P., Wolever, T.M., Vuksan, V., Mehling, C.C., Boctor, D.L., Bolognesi, C., Huang, J. & Patten, R. 1997. Effect of a diet high in vegetables, fruit, and nuts on serum lipids. Metabolism 46(5): 530-537.

Krzeminski, R., Gorinstein, S., Leontowicz, H., Leontowicz, M., Gralak, M., Czerwinski, J., Lojek, A., Číž, M., Martin-Belloso, M., Gligelmo-Miguel, N. & Trakhtenberg, S. 2003. Effect of different olive oils on bile excretion in rats fed cholesterol-containing and cholesterol-free diets. Journal of Agriculture and Food Chemistry 51(19): 5774-5779.

Lattimer, J.M. & Haub, M.D. 2010. Effects of dietary fiber and its components on metabolic health. Nutrients 2(12): 1266-1289. 

Leontowicz, H., Leontowicz, M., Gorinstein, S., Martin-Belloso, O. & Trakhtenberg, S. 2007. Apple peels and pulp as a source of bioactive compounds and their influence on digestibility and lipid profile in normal and atherogenic rats. Medycyna Wet 63(11): 1434-1436.

Libuda, L., Alexy, U., Sichert-Hellert, W., Stehle, P., Karaolis-Danckert, N., Buyken, A.E. & Kersting, M. 2008. Pattern of beverage consumption and long-term association with body weight status in German adolescents. British Journal of Nutrition 99(6): 1370-1379.

Maghrani, M., Michel, J.B. & Eddouks, M. 2005. Hypoglycaemic activity of Retama raetm in rats. Phytotherapy Research 19(2): 125-128.  

Mahmoed, M.Y. & Rezq, A.A. 2013. Hepatoprotective effect of avocado fruits against carbon tetrachloride-induced liver damage in male rats. World Applied Science Journal 21(10): 1445-1452.

McNamara, J.R., Cohn, J.S., Wilson, P.W. & Schaefer, E.J. 1990. Calculated values for low density lipoprotein cholesterol in the assessment of lipid abnormalities and coronary disease risk. Clinical Chemistry 36(1): 36-42.

Nehra, M. & Sharma, S.A. 2012. Determination of total carotenoids, total phenols, ascorbic acid content and radical scavenging activity of pomegranate, papaya and orange peels. Food Science 1(5): 55-56.

Nouri, M.K. & Rezapour, A.K. 2011. Effect of apple (Malus domestica) supplementation on serum lipids and lipoproteins level in cholesterol-fed male rat. Middle-East Journal of Scientific Research 9(6): 744-748.

Ohkawa, H., Ohishi, N. & Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95(2): 351-358.

Sallie, R., Tredger, J.M. & William, R. 1991. Drugs and the liver. Part 1: Testing liver function. Biopharmceutics and Drug Disposition 12(4): 251-259.

Shah, K.A., Patel, M.B., Shah, S.S., Chauhan, K.N., Parmar, P.K. & Patel, N.M. 2010. Antihyperlipidemic activity of Mangifera indica L. leaf extract on rats fed with high cholesterol diet. Der Pharmacia Sinica 1(2): 156-161.

Stockbridge, H., Hardy, R.I. & Glueck, C.J. 1989. Photometric determination of cholesterol (CHOD-PAP method).  Journal of Laboratory and Clinical Medicine 114: 142-151.

Thomas, L. & Labor, U. 1992. Enzymateischer kinetischer colorimetrischer test (GOD-PAP), Biocon Diagnostik, Hecke 8, 34516 Vohl/Manenhagen, Germany. Diagnose 4: 169.

Umbreen, H., Arshad, M.U., Saeed, F., Bhatty, N. & Hussain, A.I. 2015. Probing the functional potential of agro-industrial waste for dietary interventions. Journal of Food Processing and Preservation 39(6): 1665-1671.

Uppal, H., Zhai, Y., Gangopadhyay, A., Khadem, S., Ren, S., Moser, J.A. & Xie, W. 2008. Activation of liver X receptor sensitizes mice to gallbladder cholesterol crystallization. Hepatology 47(4): 1331-1342.

Wu, A.H.B. 2006. Tietz Clinical Guide to Laboratory Tests. 4th edition. Philadelphia: WB Saunders.

*Pengarang untuk surat-menyurat; email: huma_umbreen@yahoo.com