Sains Malaysiana 49(6)(2020): 1303-1312

http://dx.doi.org/10.17576/jsm-2020-4906-08

Anti-inflammatory Effect of Tamarind Seed Coat Extract against LPS-Induced RAW264.7 Macrophages
(Kesan Anti-Keradangan Ekstrak Lapisan Biji Asam Jawa terhadap Penggalak LPS RAW264.7 Makrofaj)

JIRAPA PUNTARUT¹, WIPAWADEE SIANGLUM², SUPITA TANASAWET³, PENNAPA CHONPATHOMPIKUNLERT⁴ & WANIDA SUKKETSIRI¹*

¹Department of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, 90110 Thailand

²Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, 90110

Thailand

³Department of Anatomy, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand

⁴Expert Center of Innovative Health Food, Thailand Institute of Scientific and Technological Research (TISTR), Pathumthani, 12120, Thailand

Diserahkan: 2 Ogos 2019/ Diterima: 17 Februari 2020

ABSTRACT

Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL^-1) were applied and then stimulated with LPS (1 μg mL^-1) in RAW264.7 macrophages and the level of reactive oxygen species (ROS) and nitric oxide (NO) were measured. Besides, enzyme-linked immunosorbent assay (ELISA) was used to measure the level of pro-inflammatory cytokines. Our results showed that TSCE suppressed LPS-induced intracellular ROS production and suppressed the NO levels in a dose-dependent manner. Significantly, the anti-inflammatory activity was correlated with a lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines. These results implied that TSCE possess potent anti-inflammatory activity, which supported new insights into the TSCE utilization to protect inflammation-related disorders.

Keywords: Interleukin-1β; reactive oxygen species; Tamarindus indica; tumor necrosis factor alpha

ABSTRAK

Tindak balas keradangan dimodulasi oleh sel-sel imun yang dirangsang dan memainkan peranan penting dalam sistem pertahanan perumah terhadap pelbagai rangsangan. Dalam kajian ini, kami menilai sifat anti-radang daripada ekstrak lapisan biji asam jawa (TSCE) pada lipopolisakarida (LPS) yang diaruh makrofaj RAW264.7. Pelbagai kepekatan TSCE (10, 25 dan 50 µg mL^-1) digunakan dan kemudian dirangsang dengan LPS (1 μg mL^-1) pada makrofaj RAW264.7 dan tahap spesies oksigen reaktif (ROS) dan nitrik oksida (NO) diukur. Selain itu, asai imunosorben berkait enzim (ELISA) digunakan untuk mengukur tahap sitokin pro-radang. Hasil kajian menunjukkan bahawa TSCE menindas pengeluaran intraselular ROS yang disebabkan oleh LPS serta menindas aras NO bergantung pada dos atau kepekatan. Aktiviti anti-radang berkorelasi dengan penurunan LPS yang merangsang sitokin pro-radang TNF-α dan IL-1β secara ketara. Hasil ini menunujukkan bahawa TSCE memiliki aktiviti anti-radang yang tinggi sekaligus menyokong pandangan baru tentang penggunaan TSCE dalam melindungi gangguan yang berkaitan dengan keradangan.

Kata kunci: Faktor nekrosis tumor alfa; interleukin-1β; spesies oksigen reaktif; Tamarindus indica

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*Pengarang untuk surat-menyurat; email: wanida.su@psu.ac.th