Sains Malaysiana 51(1)(2022): 1-13

http://doi.org/10.17576/jsm-2022-5101-01

Molecular Identification and Ochratoxigenic Potential of Black Aspergillus from Various Substrates and Indoor Environment

(Pengenalpastian Molekul dan Potensi Okratoksigen Aspergillus Hitam daripada Pelbagai Substrat dan Persekitaran Dalaman)

WARDAH ABDUL RAHMAN & LATIFAH ZAKARIA*

School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

Diserahkan: 28 Julai 2020/Diterima: 17 Mei 2021

ABSTRACT

Aspergillus section Nigri or black Aspergillus is characterized by dark brown to black colonies. Several species of this group showcase the ability to produce ochratoxin A (OTA), contaminating various types of food and feed. This study was conducted to identify black Aspergillus isolated from rice, groundnuts, spices, corn grains, soil, and indoor environment and determine the ability of the isolates to produce OTA. Based on ITS, β-tubulin and calmodulin sequences, 177 isolates of black Aspergillus from various substrates and indoor environment were identified as A. niger, A. aculeatus, and A. tubingensis. By using two sets of primers to amplify two ochratoxin biosynthesis genes (PKS15KS and PKS15C-MeT genes), the OTA genes were detected in only six A. niger isolates from rice and indoor environment. These six A. niger isolates produced 8 to 308 µg/g of OTA as quantified using UPLC analysis. No OTA gene was detected in any of the A. tubingensis and A. aculeatus isolates. In conclusion, three black Aspergillus species, A. niger, A. aculeatus, and A. tubingensis, were identified from rice, groundnuts, spices, corn grains, soil, and indoor environment. Only six isolates of A. niger produced OTA indicating most of the A. niger isolates were non-OTA producers. These results could thus portray the occurrence of OTA in the field. Since both the number of isolates producing OTA and the levels of OTA production were low, it could be possibly assumed that the occurrence as well as the levels of OTA in the field are also low.

Keywords: Aspergillus aculeatus; A. niger; A. tubingensis; black Aspergillus; ochratoxin A

ABSTRAK

Aspergillus seksyen Nigri atau Aspergillus hitam dicirikan oleh koloni perang gelap ke hitam. Beberapa spesies kumpulan ini mempamerkan keupayaan menghasilkan okratoxin A (OTA) yang dapat dikesan dalam pelbagai jenis makanan dan makanan ternakan. Pengenalpastian ke tahap spesies Aspergillus hitam agak sukar kerana persamaan ciri makroskopik dan ciri mikroskopik. Dalam kajian ini, Aspergillus hitam dipencilkan daripada bahan makanan (beras, kacang tanah rempah dan bijirin jagung), tanah dan persekitaran dalaman. Sebanyak 177 pencilan ini dikenal pasti sebagai A. niger, A. aculeatus dan A. tubingensis berdasarkan jujukan kawasan transkripsi penjarak dalaman (ITS), β-tubulin dan kalmodulin. Gen OTA hanya dikesan dalam enam pencilan A. niger yang diperoleh daripada beras dan persekitaran dalaman. Pencilan A. niger ini menghasilkan 8 hingga 308 µg/g OTA yang dikuantifikasi menggunakan analisis UPLC. Tiada gen OTA yang dikesan dalam pencilan A. tubingensis dan A. aculeatus. Kesimpulannya, tiga spesies Aspergillus hitam, A. niger, A. aculeatus dan A. tubingensis dikenal pasti daripada beras, kacang tanah, rempah, bijirin jagung, tanah dan persekitaran dalaman. Hanya 4.2% daripada pencilan A. niger menghasilkan OTA dan ini menunjukkan kebanyakan pencilan A. niger bukan pengeluar OTA. Keputusan kajian dapat menggambarkan kejadian OTA di lapangan. Oleh kerana bilangan pencilan yang menghasilkan OTA dan tahap penghasilan OTA adalah rendah, kemungkinan kejadian dan tahap OTA di lapangan juga adalah rendah.

Kata kunci: Aspergillus aculeatus; Aspergillus hitam; A. niger; A. tubingensis; okratoksin A.

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*Pengarang untuk surat-menyurat; email: Lfah@usm.my