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Sains Malaysiana 51(7)(2022):
http://doi.org/10.17576/jsm-2022-5107-01
Elucidating Hepatic
Lipidosis in Stray Cats through Serum Biochemistry, Liver Histopathology and Liver
RNA Expression of PPAR-δ and PPAR-γ
(Pengesanan Lipodesis Hepar pada Kucing Liar melalui Serum Biokimia, Histopatologi Hati dan Ekspresi RNA Hati PPAR- δ dan PPAR- γ)
1Department of Veterinary Preclinical Science, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor Darul Ehsan, Malaysia
2Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
3Department of Veterinary Pathology & Microbiology, Faculty of
Veterinary Medicine, Universiti Putra Malaysia, 43400
UPM Serdang, Selangor Darul Ehsan, Malaysia
4Institute of Biological Sciences, Faculty of Science, University of
Malaya, Kuala Lumpur, Wilayah Persekutuan, Malaysia
5Natural Medicines and Products Research Laboratory (NaturMeds), Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
6Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, 16100 Kota Bharu, Kelantan Darul Naim, Malaysia
7Jade
Hills Veterinary Hospital, Jade Hills, 43000 Kajang,
Selangor Darul Ehsan, Malaysia
8Beaty
Water Research Centre, Queen’s University, Kingston, Ontario, Canada
9School
of Environmental Studies & Department of Chemistry, Queen’s University,
Kingston, Ontario, Canada
Dataran Jade, 28, Jln Dataran Jls, 43000 K Dataran Jade, 28, Jln Dataran Jade 2,
Diserahkan: 10 Oktober 2021/Diterima:
2 Januari 2022
ABSTRACT
Early
detection of feline hepatic lipidosis (FHL) with appropriate treatment can
increase prognosis significantly. This study looks into the serum biochemistry
and lipid composition of serum and liver samples in a group of stray cats
(N=18) collected from pounds in Klang Valley, Malaysia. Alanine aminotransferase
(ALT) in blood serum was used to detect for liver damage possibly due to FHL,
confirmed through light microscopy, serum biochemistry (triglyceride,
cholesterol, creatinine, and urea), liver triglyceride and cholesterol
concentrations, and liver RNA expression of lipid droplet regulators peroxisome
proliferator-activated receptors (PPARs). Differing severity of FHL in samples
were divided and grouped using an adapted scoring method observing fatty change
of liver (FCL) with trends between FCL groups investigated. Elevated serum ALT
reflective of increasing FCL severity was observed with elevated concentrations
of liver TAG and cholesterol levels. Serum TAG and cholesterol decreased with
heightened FCL pointing to fatty acid oxidation and lipid restoration in the
liver, supported by PPAR-γ expression which also propose macrophage
activation for liver recovery alongside PPAR-δ for lipogenesis and
inflammatory reactions. Elevated serum creatinine and urea levels with
increasing FCL severity propose overall intact hepatic function in the stray
cat samples.
Keywords: Felis catus;
hepatic lipidosis; PPAR-δ; PPAR-γ; serum biochemistry
Abstrak
Pengesanan awal lipidosis hepatik felin (LHF) berserta rawatan bersesuaian
dapat meningkatkan prognosis dengan ketara. Kajian ini meneliti serum biokimia
dan komposisi serum lipid dan sampel hati sekumpulan kucing liar (N=18) yang telah
dikumpul daripada pusat lindungan kucing terbiar di sekitar Lembah Klang,
Malaysia. Alanina aminotransferase (ALT) di dalam serum darah digunakan bagi
mengesan kerosakan hati yang mungkin disebabkan LHF, disahkan melalui
mikroskopi cahaya, serum biokimia (trigliserida, kolesterol, kreatinina dan
urea), kandungan trigliserida (TG) dan kolestrol hati serta pengekspresan asid
ribonukleik hati daripada pengatur titik lipid reseptor-reseptor
diaktifkan-pengproliferat peroksisom (RDPP) proliferator peroksisom -diaktifkan
reseptor (PPARs). Keparahan yang berbeza antara sampel LHF dibahagi dan
dikelompok menggunakan sebuah kaedah penilaian yang telah digubah untuk
memerhati perubahan lemak hati (PLH), seterusnya mengkaji trend PLH antara
kelompok. Kenaikan serum ALT seiring dengan keparahan PLH telah diperhati berserta
kenaikan kandungan TG dan kolesterol hati. Penurunan serum TG dan kolesterol
seiring dengan keparahan PLH menunjukkan berlakunya pengoksidaan asid lemak dan
pemulihan lipid di dalam hati, ini telah disokong oleh pengekspresan
RDPP-γ yang juga mencadangkan pengaktifan makrofaj bagi memulihkan hati di
samping RDPP-δ untuk lipogenesis dan reaksi-reaksi radang. Kenaikan
kreatinina dan serum urea seiring dengan keparahan PLH mencadangkan kebolehan
fungsi hepatik pada kucing-kucing terbiar yang dikaji.
Kata kunci: Felis catus; lipodesis hepar; PPAR-δ;
PPAR-γ; serum biokimia
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