Sains Malaysiana 51(7)(2022): 2329-2337

http://doi.org/10.17576/jsm-2022-5107-31

Jumlah Fenol, Aktiviti Antioksidan dan Ketoksikan Ekstrak Bendalir Lampau Genting (SFE)

Buah Ziziphus mauritiana

(Total Phenol, Antioxidant Activity and Toxicity of Supercritical Fluid (SFE) Extract of Ziziphus mauritiana Fruit)

ARNIDA HANI TEH^1,2,*, MUHIYUDDIN MD NASIR¹, SAIDATUL HUSNI SAIDIN³ & HAFEEDZAABDUL RAHMAN^1,2

¹Department of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
²Innovation Center for Confectionary Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

³Herbal Product Development Programme, Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia

Diserahkan: 25 Februari 2022/Diterima: 18 April 2022

Abstrak

Kajian ini menentukan jumlah fenol, antioksidan dan ketoksikan buah Ziziphus mauritiana yang diekstrak menggunakan pengekstrakan bendalir lampau genting (SFE). Ekstrak dianalisis untuk jumlah kandungan fenol (TPC) dan aktiviti antioksidan berdasarkan aktiviti penyingkiran radikal bebas, 2,2-difenil-1-pikrilhidrazil (DPPH). Kajian ketoksikan akut dilakukan pada tikus Sprague Dawley (24 jantan, 24 betina), dibahagikan kepada 4 kumpulan; diberikan 0 (kawalan), 1, 2.5 dan 5 g ekstrak/kg berat badan. Kajian kesitotoksikan dilakukan menggunakan ujian MTT pada titisan sel V79-4 dan kajian kegenotoksikan dilakukan menggunakan Ujian Mikronukleus (MNA) dengan pewarnaan akridin jingga (AO). Ekstrak menghasilkan TPC sebanyak 539.18 mg setaraan asid galik (GAE)/100 g dan penyingkiran radikal bebas DPPH sebanyak 16.65%. Dalam kajian ketoksikan akut, semua tikus selamat tanpa bukti manifestasi klinikal atau ketoksikan. Kajian kesitotoksikan ekstrak menunjukkan kesan kesitotoksikan (IC₅₀ 0.48 mg/mL). Kajian kegenotoksikan menunjukkan bahawa ekstrak tidak mempunyai kesan genoketoksikan pada tikus yang diuji. Secara keseluruhan, ekstrak bertindak dengan cara yang berbeza, in vivo dan in vitro. Sebagai kesimpulan, ekstrak adalah sumber fenol yang baik namun tidak menunjukkan aktiviti antioksidan yang tinggi. Ia tidak toksik dan tidak menyebabkan kegenotoksikan terhadap haiwan yang dikaji pada kepekatan sehingga 5 g ekstrak/kg berat badan. Walau bagaimanapun, ekstrak didapati sitotoksi terhadap titisan sel V79-4 yang dikaji.

Kata kunci: Antioksidan; kegenotoksikan; kesitotoksikan; ketoksikan akut; pengekstrakan bendalir lampau genting; Ziziphus mauritiana

Abstract

This study determined the total phenolics, antioxidant and toxicity of Ziziphus mauritiana fruit extracted using supercritical fluid extraction (SFE). The extract was analysed for the total phenolic content (TPC) and antioxidant activity based on radical scavenging activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH). Acute toxicity studies were done in Sprague Dawley rats (24 males, 24 females), divided into 4 groups; receiving 0 (control), 1, 2.5 and 5 g extract/kg body weight. Cytotoxicity studies were done using MTT assay on V79-4 cell lines, and genotoxicity studies were done using Micronucleus Assay (MNA) with acridine orange (AO) staining. The extract produced TPC of 539.18 mg gallic acid equivalent (GAE)/100 g and DPPH scavenging of 16.65 %. In the acute toxicity studies, all rats survived with no evidence of clinical or toxic manifestation. Cytotoxicity studies showed that the extract demonstrated a cytotoxic effect (IC₅₀ 0.48 mg/mL). Genotoxicity studies showed that the extract had no genotoxic effect on the tested rats. Overall, the extract behaves in different manner, in vivo and in vitro. As a conclusion, the extract is a good source of phenolics, however, does not show high antioxidant activity. It is non-toxic and not causing genotoxicity towards the studied animals at the concentration up to 5 g extract/kg body weight. However, the extract is found to be cytotoxic towards the V79-4 cell lines studied.

Keywords: Acute toxicity; antioxidant; cytotoxicity; genotoxicity; supercritical fluid extraction; Ziziphus mauritiana

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^*Pengarang untuk surat-menyurat; email: arnida@ukm.edu.my