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Sains Malaysiana 51(9)(2022):
http://doi.org/10.17576/jsm-2022-5109-14
A Simple Potentiometric Biosensor Based on Carboxylesterase for the
Analysis of Aspartame
(Biosensor Potensiometrik Mudah Berasaskan
Karboksilesterase untuk Analisis Aspartam)
NOOR IZAANIN RAMLI1, LEE YOOK HENG1,2,* & LING LING TAN2
1Jabatan Sains Kimia, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia,
43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
2Pusat
Kajian Bencana Asia Tenggara (SEADPRI-UKM), Institut Alam Sekitar dan Pembangunan (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi,
Selangor Darul Ehsan, Malaysia
Diserahkan: 1 Disember 2021/Diterima: 13 Mac 2022
Abstract
A
potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase
(CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)]
microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH
transducer was made from non-plasticized polyacrylate membrane containing a
hydrogen ionophore and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic
acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of
aspartame concentration was performed by quantifying the hydrogen ion
concentration produced from L-Asp. The potentiometric determination of
aspartame exhibited good selectivity with near Nernstian response. The
sensitivity of the biosensor was closed to the Nernstian value, i.e., 50-52 mV
decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching 10-6 M. The
aspartame biosensor demonstrated good repeatability and reproducibility with
relative standard deviation (RSD) of 1.9% and 1.6%, respectively (n=3).
The potentiometric aspartame biosensor was demonstrated to be reliable for
determining aspartame content in sweetener samples and was comparable to the
conventional high-performance liquid chromatography (HPLC) method for aspartame
analysis.
Keywords: Acrylic microspheres; apartamepotentiometric;
Nernst; screen-printed electrode
Abstrak
Biosensor aspartam
potensiometri telah dibangunkan dengan hanya meletakkan mikrosfera poli(n-butil
akrilat-n-akriloksisuksimida) terikat karboksilesterase (CES) [CES-poli(nBA-NAS)]
pada elektrod terpilih pH cetakan skrin Ag/AgCl. Transduser pH disediakan
daripada membran poliakrilat bukan plastik yang mengandungi ionofor hidrogen
dan garam lipofilik. Enzim CES terpegun memangkinkan hidrolisis enzimatik
aspartam kepada asid L-aspartik (L-Asp), L-phenylalanine dan metanol. Penentuan
potensiometrik kepekatan aspartam dilakukan dengan mengukur kepekatan ion
hidrogen yang dihasilkan daripada L-Asp. Penentuan potensiometrik aspartam
menunjukkan kepilihan yang baik dengan rangsangan Nernstian yang hampir.
Kepekaan biosensor adalah berhampiran dengan nilai Nernstian, iaitu 50-52
mV dekad-1 dengan julat tindak balas linear dinamik dari 10-5 hingga 10-2 M dan had pengesanan menghampiri 10-6 M.
Biosensor aspartam menunjukkan kebolehulangan dan kebolehasilan yang baik
dengan sisihan piawai relatif (RSD) masing-masingnnya sebanyak 1.9% dan 1.6% (n=3).
Biosensor aspartam potensiometrik telah ditentusahkan dalam penentukan
kandungan aspartam dalam sampel pemanis dan setanding dengan kaedah kovensional
kromatografi cecair berprestasi tinggi (HPLC) untuk analisis aspartam.
Kata kunci: Aspartam; elektrod bercetak skrin; mikrosfera akrilik; Nernst;
potensiometrik
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*Pengarang untuk surat-menyurat; email: leeyookheng@yahoo.co.uk
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