The Malaysian Journal of Analytical Sciences
Vol 12 No 2 (2008): 444 – 450
COMPARISON OF DIFFERENT TYPES OF COATINGS IN
HEADSPACE SOLID PHASE MICROEXTRACTION FOR THE ANALYSIS OF PESTICIDE RESIDUES IN
VEGETABLES AND FRUITS
Chai Mee Kin1* and
Tan Guan Huat2
1Dept. of Science and Mathematics, College of
Engineering,
Universiti Tenaga Nasional, Km 7, Jalan Kajang-Puchong,
43009 Kajang, Selangor.
2Dept. of Chemistry, Faculty of Science,
Universiti Malaya, Lembah Pantai,
50603 Kuala Lumpur.
*Corresponding
author: Mkchai@uniten.edu.my
Abstract
Despite
the continuing development of solid-phase microextraction
(SPME) fiber coatings, their selection presents some difficulties for analytes in choosing the appropriate fiber for a particular
application. There are many types of SPME coatings available commercially. The
most widely used for determination of pesticide residues in vegetable and
fruits are polydimethylsiloxane (PDMS) and polyacrylate (PA). A headspace solid phase microextraction (HS-SPME) procedure using these two
commercialized fibers (PDMS and PA) is presented for the determination of
selected groups of organochlorine and organophosphorus pesticides. The extraction performances of
these compounds were compared using these two fibers. The optimal experimental
procedures for the adsorption and desorption of pesticides were determined. An
explanation for the extraction differences is suggested based on the different
thickness, polarity of the polymeric film of fibers and the different
extracting matrices. In addition, the higher detector response
of the pesticides after addition of aliquots of water and an organic solvent to
the vegetable and fruit samples are also discussed. The SPME fibers were
re-usable until a maximum of 120 extractions.
Finally, the optimized procedures were applied successfully for the
determination of these compounds in vegetable and fruits samples. Mean recoveries for all pesticides were
between 75.0-97% with RSD below 7%.
Keywords: HS-SPME,
GC-ECD, GC-MS, pesticide.
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