The Malaysian Journal of Analytical Sciences
Vol 12 No 2 (2008): 473 – 479
BIOPENDERIA
ELEKTROKIMIA BERASASKAN ENZIM ALKALINE
FOSFATASE TERPEGUN UNTUK PENGESANAN KETOKSIKAN ASID
2,4-DIKLOROFENOKSIASETIK
(An
Electrochemical Biosensor Based on the Immobilisation of Alkaline Phosphatase
for the Determination of Toxicity of 2,4-Dichlorophenoxylacetic Acid)
Loh Kee Shyuan1,
Lee Yook Heng1*, Musa Ahmad1, Salmah Abdul Aziz2
& Zamri Ishak2
1Pusat Pengajian Sains Kimia & Teknologi Makanan,
Fakulti Sains & Teknologi,
Universiti Kebangsaan Malaysia (UKM), 43600 Bangi,
Selangor
2 Pusat Penyedilikan Bioteknologi, Institut
Penyelidikan & Kemajuan Pertanian Malaysia (MARDI),
P.O. BOX 12301, GPO Kuala Lumpur
*Corresponding
author: yhl1000@ukm.my
Abstract
An
electrochemical biosensor based on the immobilisation
of alkaline phosphatase was developed for the
determination of 2,4-dichlorophenoxylacetic acid
(2,4-D). The biosensor was constructed from the immobilization of alkaline phosphatase enzyme onto a screen-printed electrode (SPE).
The ascorbic acid 2-phosphate (AA2P) was used as substrate for the enzymic reaction. The enzyme was entrapped in a hybrid
sol-gel/chitosan material with certain fixed
composition. The determination of toxicity of 2,4-D
pesticides quantitatively and qualitatively could be carried out by the
inhibition of the alkaline phosphatase. A potential
of +600 mV was suitable to be used for the oxidation of the products from the
enzyme-substrate reaction, where the reaction pH was at 8.5. The linear response
range of the biosensor to the AA2P substrate was 10 mM-80 mM. The inhibition
of the alkaline phosphatase enzyme of the 2,4-D biosensor was maximum at 80 ppm
2,4-D (50% inhibition).
Keywords: Electrochemical biosensor, alkaline phosphatase, 2,4-dichlorophenoxylacetic
acid, sol-gel, chitosan
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