Sains Malaysiana 37(4): 365-371(2008) 

Isolation and Identification of Marine Sulphate-Reducing Bacteria,

Desulfovibrio sp. and Citrobacter freundii from

Pasir Gudang, Malaysia

(Pemencilan Bakteria Penurun-Sulfat Marin, Desulfovibrio sp. dan

Citrobacter freundii dari, Pasir Gudang, Malaysia)

Fathul Karim Sahrani, Zaharah Ibrahim,  Adibah Yahya & Madzlan Aziz 

School of Environment and Natural Resource Sciences

Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor, Malaysia

Recieved:   3 April 2007 /Accepted:   6 December 2007 

ABSTRACT 

In the attempt to isolate indigenous marine sulphate-reducing bacteria from coastal samples, we obtained some swarm forming bacteria. The isolates were screening using Starkey’s medium and detection of main groups of SRB were carried out using commercially kits (SRB BART kits, Droycon Bioconcepts Inc., Canada). From the growth characteristic, only two isolates were strongly suggested as marine SRB. Based on the 16S rRNA gene sequence analysis these SRB were closely related and could be designated as Desulfovibrio sp. and Citrobacter freundii, with the highest sequence similarity of 98% and 93%, respectively.

Key words: Sulphate-reducing bacteria; SRB BART kits; 16S rRNA sequence

ABSTRAK

Dalam usaha untuk memencilkan bakteria penurun-sulfat (SRB) yang asli dari sampel persekitaran marin, kami memperoleh beberapa kumpulan koloni bakteria. Pemencilan seterusnya disaring menggunakan medium Starkey’s dan pengesanan kumpulan utama sebagai SRB dijalankan menggunakan kit komersial SRB (SRB BART kits, Droycon Bioconcepts Inc., Canada). Daripada pencirian pertumbuhan didapati hanya dua pemencilan dicadangkan sebagai SRB marin.  Analisis urutan 16S rRNA menunjukkan bahawa SRB tersebut sangat rapat dan boleh dipadankan sebagai Desulfovibrio sp. dan Citrobacter freundii dengan kesamaan masing-masing 98% dan 93%.

Kata kunci : Bakteria penurun-sulfat; kit BART SRB; urutan 16S rRNA

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