Sains Malaysiana 39(1)(2010): 119–128

 

Optimization of Extraction Parameters of Total Phenolic Compounds

from Henna (Lawsonia inermis) Leaves

(Pengoptimuman Parameter Pengekstrakan Jumlah Sebatian Fenolik

daripada Daun Inai (Lawsonia inermis))

 

D.B. Uma & C.W. Ho*

Department of Food Science and Nutrition, Faculty of Applied Sciences

University College Sedaya International (UCSI), No. 1, Jalan Menara Gading

UCSI Heights, 56000 Kuala Lumpur, Malaysia

 

W.M. Wan Aida

School of Chemical Sciences and Food Technology

Faculty of Science and Technology, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor D.E., Malaysia

 

Received: 5 November 2008 / Accepted: 17 March 2009

 

ABSTRACT

 

Response surface methodology (RSM) in conjunction with central composite rotatable design (CCRD) was performed in the present study to optimize the extraction parameters for assessing maximum yield of total phenolic content (TPC) from henna (Lawsonia inermis) leaves. The range of the independent variables, namely acetone concentration (20-90%, v/v), extraction time (10-90 minutes) and extraction temperature (25-45 C) were identified by a first set of single factor experiments. The actual values of the independent variables coded at five levels were selected based on the results of single factor experiments. The optimum conditions for extraction of TPC were found to be at acetone concentration 48.07%, extraction time 73.78 minutes and extraction temperature 39.57 C. Under these optimized conditions, the experimental maximum yield of TPC was 7203.74 mg GAE/100g DW, which was in close agreement with predicted values, thus indicating the suitability of the models developed and the success of RSM in optimizing the extraction conditions.

 

Keywords: Central composite rotatable design; Henna (Lawsonia inermis) leaves; response surface methodology; total phenolic content

 

ABSTRAK

 

Kaedah Permukaan Respons (RSM) bersama-sama reka bentuk komposit putaran tengah (CCRD) telah dijalankan dalam kajian ini untuk mengoptimumkan parameter pengekstrakan bagi penentuan jumlah sebatian fenolik (TPC) daripada daun henna (Lawsonia inermis). Julat parameter tak bersandar iaitu kepekatan pelarut aseton, (20-90% v/v), masa pengekstrakan (10-90 minit) dan suhu pengekstrakan (25-45 C) dikenal pasti daripada kajian awalan uji kaji faktor tunggal. Nilai sebenar parameter tak bersandar yang berkod pada lima aras telah dipilih berdasarkan ujikaji faktor tunggal tersebut. Keputusan menunjukkan parameter optimum untuk mengekstrak TPC adalah pada kepekatan aseton, 48.07%; masa pengekstrakan, 73.78 minit dan suhu pengekstrakan, 39.57 C. Dalam keadaan optimum pengekstrakan ini, nilai eksperimen bagi jumlah sebatian fenolik (TPC) maksimum ialah 7203.74 mg GAE/100g berat kering yang sepadan dengan nilai yang diramal menunjukkan model yang dibangunkan menggunakan kaedah RSM adalah sesuai.

 

Kata kunci: Daun inai (Lawsonia inermis); jumlah sebatian fenolik; kaedah permukaan respons; reka bentuk komposit putaran tengah

 

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*Corresponding author; email: cwho@ucsi.edu.my