Sains Malaysiana 42(1)(2013): 33–38

Plasma Fibronectin in Saliva of Women with History of Miscarriage(s)

and Periodontal Disease - a Pilot Study

(Fibronektin Plasma di dalam Air Liur Wanita Mengandung dengan Riwayat Keguguran

dan Penyakit Periodontitis Kronik- Kajian Permulaan)

Farha Ariffin*

Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

Salmah Kamin

KPJ Selangor Specialist Hospital, 40300 Shah Alam, Selangor, Malaysia

Jamiyah Hassan

Department of obstetrics and gynaecology, University of Malaya Medical Centre

50900 Kuala Lumpur, Malaysia

Haliza Hasan

Faculty of computer Science and Mathematics, Universiti Teknologi MARA

40450 Shah Alam, Selangor, Malaysia

Received: 19 December 2011/Accepted: 18 May 2012

ABSTRACT

The objective of this study was to establish the association of plasma fibronectin in saliva of pregnant women with history of miscarriage(s). A total of 25 pregnant women with history of miscarriage(s) were recruited at the Antenatal Clinic of University Malaya Medical Centre (UMMC), Kuala Lumpur as the test group and 16 pregnant women without any history of miscarriage served as control. All the subjects fulfilled a set of inclusion and exclusion criteria. Periodontal examination was performed to determine subjects’ periodontal status. Saliva samples were collected and enzyme-linked immunosorbent assay (ELISA) was used to detect the plasma fibronectin in these samples. Mann-Whitney test was used to determine the significance of differences seen. The study showed a statistically significant higher median plasma fibronectin level in the saliva samples of the test group with a median level of 0.10 μg/mL compared with the control group with a mean value of 0.00 μg/mL (p-value =0.023). However, there was no significant difference in the level of plasma fibronectin in the saliva samples of pregnant women with chronic periodontitis compared with the group with healthy gingiva (p-value= 0.118). As a conclusion, there was a positive association between plasma fibronectin in saliva of women and history of miscarriages compared with the control group in this case study.

Keywords: Miscarriage; periodontal disease; plasma fibronectin; saliva

ABSTRAK

Objektif kajian ini adalah untuk melihat hubung kait antara fibronektin plasma di dalam air liur ibu mengandung dengan sejarah keguguran. Seramai 25 ibu mengandung dengan sejarah keguguran di Klinik Ibu Mengandung Pusat Perubatan Universiti Malaya, Kuala Lumpur telah dikenal pasti sebagai kumpulan kajian manakala seramai 16 lagi ibu mengandung tanpa riwayat keguguran dijadikan kumpulan kawalan. Kesemua subjek kajian menepati kriteria yang telah ditetapkan. Pemeriksaan periodontium telah dilakukan bagi mengenal pasti status kesihatan periodontium mereka. Sampel saliva mereka dianalisis menggunakan ujian ‘enzyme-linked immunosorbent assay’ (ELISA) untuk mengesan kandungan fibronektin plasma. Ujian Mann-Whitney digunakan bagi menganalisis data. Keputusan mendapati aras purata fibronektin plasma di dalam kumpulan kajian adalah melebihi berbanding purata fibronektin plasma dalam kumpulan kawalan. Walau bagaimanapun, tiada perbezaan ketara yang ditemui bagi purata fibronektin plasma antara kumpulan subjek yang mempunyai penyakit periodontitis kronik berbanding subjek yang mempunyai periodontium yang sihat. Kesimpulannya, kajian ini menunjukkan bahawa terdapat hubung kait yang positif antara aras fibronektin plasma di dalam saliva subjek dengan riwayat keguguran.

Kata kunci: Air liur; fibronektin plasma; keguguran; penyakit periodontium

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*Corresponding author; email: drfarha@salam.uitm.edu.my