Sains Malaysiana 42(2)(2013): 223–229

A Novel Colorimetric Sensing Material, Poly(γ-Glutamic acid)-graft-3, 4-Dihydro-3-(2′-ethyl hydroxyl)-6-Ethyl-1,3,2H-Benzoxazine (γ-PGA-graft-ethyl-Bx), for Iron(III) Ions

(Bahan Pengesan Kolorimetri Baru, Poly(γ-Asid Glutamic)-graf-3,

4-Dihidro-3-(2′-etil hidroksil)-6-Etil-1,3,2H-Benzoksazin

(γ-PGA-graf-etil-Bx) untuk Iron Ferum (III))

Nu-orn Choothong, Attaphon Kaewvilai, Apirat Laobuthee* & Amornrat Lertworasirikul

Department of Materials Engineering, Faculty of Engineering

Kasetsart University, Bangkok 10900, Thailand

Received: 7 January 2012 / Accepted: 21 May 2012

ABSTRACT

A novel rapid ion colorimetric sensing material for iron(III) ions was developed from poly(γ-glutamic acid) and 3,4-dihydro-3-(2′-ethyl hydroxyl)-6-ethyl-1,3,2H-benzoxazine (ethyl-Bx). The benzoxazine as an ionophore segment was grafted into γ-PGA backbone via the esterification reaction, which is a simple and effective reaction. The structure of γ-PGA-graft-ethyl-Bx was characterized by using FT-IR and 1H-NMR. The most attainable degree of conversion was 25%. The γ-PGA-graft-ethyl-Bx showed a highly selective and sensitive recognition toward iron(III), which was clearly observable with the naked eye. The iron(III) ions sensing property of γ-PGA-graft-ethyl-Bx was further examined by using photometric titration method. After the interaction between the γ-PGA-graft-ethyl-Bx and iron(III) ions was formed, the solution of the polymer in dimethyl sulfoxide was changed from clear and colorless to red color, resulting in the shift of the maximum wavelength from UV to visible range.

Keywords: Benzoxazine; colorimetric sensing materials; photometric titration method; transition metal ion;γ-PGA

ABSTRAK

Bahan sensor kolorimetrik ion pantas yang baru untuk ion ferum(III) telah dihasilkan daripada asid poli(γ-glutamat) dan 3,4-dihidro-3-(2’-etil hidroksil)-6-etil-1,3,2H-benzoksazin (etil-Bx). Benzoksazin yang bertindak sebagai segmen ionofor telah digabungkan dengan struktur utama γ-PGA melalui tindak balas esterifikasi, yang merupakan tindak balas mudah dan berkesan. Struktur γ-PGA-graf-etil-Bx dikelaskan dengan menggunakan FT-IR dan 1H-NMR. Perubahan yang paling tinggi boleh dicapai adalah 25%. γ-PGA-graf-etil-Bx menunjukkan keserasian yang sangat memilih dan sensitif terhadap ferum(III), yang boleh diamati dengan mata kasar. Sifat penderiaan ion ferum (III) bagi γ-PGA-graf-etil-Bx telah dikaji dengan lebih lanjut menggunakan kaedah pentitratan fotometri. Setelah interaksi antara γ-PGA-graf-etil-Bx dan ion ferum (III) terbentuk, larutan polimer dalam dimetil sulfoksida telah berubah daripada jernih dan tidak berwarna kepada warna merah, lantas menghasilkan perubahan gelombang yang maksimum daripada UV kepada julat yang boleh dilihat.

Kata kunci: γ-PGA; bahan sensor kolorimetrik; benzoksazin; ion logam transisi; kaedah titrasi fotometri

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*Corresponding author; email: fengapl@ku.ac.th