Sains Malaysiana 42(6)(2013): 697–706

 

Antioxidant Activity of Methanol Extract of Tinospora crispa

and Tabernaemontana corymbosa

(Aktiviti Antioksida Ekstrak Metanol Tinospora crispa dan Tabernaemontana corymbosa)

 

 

Heida Nadia Zulkefli, Jamaludin Mohamad* & Nurhayati Zainal Abidin

Institute of Biological Sciences, Faculty of Science, University of Malaya

50603 Kuala Lumpur, Malaysia

 

Received: 30 November 2011/Accepted: 16 December 2012

 

 

ABSTRACT

Tinospora crispa and Tabernaemontana corymbosa have been used traditionally to treat fever, diabetes, rheumatism and sinusitis. The objective of this study was to evaluate the antioxidant activity of Tinospora crispa and Tabernaemontana corymbosa. The presence of apigenin and magnoflorine was detected using LCMS/MS in Tinospora crispa (Patawali) whereas appararicine, voafinine, conodusarine, conodurine, voacamine and voacangine were detected in Tabernaemontana corymbosa (Susur kelapa) methanol extract. The stem extract of Tinospora crispa showed high antioxidant activity in the following order: DPPH radical scavenging, reducing power and metal chelating assay (98.8%, 0.957, 81.97%) than Tabernaemontana corymbosa of leaves (90.04%, 0.652, 69.64%), stem (82.78%, 0.819, 36.70%) and root extracts (63.25%, 0.469, 51.56%), respectively. The high antioxidant activity in the stem extract of Tinospora crispa is due to the presence of apigenin and magnoflorine. The high antioxidant activity in Tabernaemontana corymbosa extract is due to its high phenol contents. There were significant linear positive correlation (r=0.788, p<0.001, r2=0.621) between the total phenolic content and DPPH free radical scavenging assay in the crude extracts of Tinospora crispa and Tabernaemontana corymbosa. Meanwhile, a significant moderate positive correlation was observed between the total phenolic content and ferric reducing power assay (r= 0.556, p<0.05, r2= 0.309). However, there was no significant difference in the correlation coefficient of total phenolic content and metal chelating assay.

 

Keywords: Antioxidant activity; Tabernaemontana corymbosa; Tinospora crispa; total phenolic content

 

ABSTRAK

Tinospora crispa dan Tabernaemontana corymbosa telah digunakan dalam perubatan tradisi untuk merawat demam, diabetik, reumatisme dan sinusitis. Objektif kajian ini adalah untuk menilai aktiviti antioksida dalam Tinospora crispa dan Tabernaemontana corymbosa. Kehadiran apigenin dan magnoflorine telah dikesan menggunakan LCMS/MS di dalam Tinospora crispa (Patawali) manakala appararicine, voafinine, conodusarine, conodurine, voacamine dan voacangine telah dikesan di dalam Tabernaemontana corymbosa(Susur kelapa) ekstrak methanol. Ekstrak batang Tinospora crispa menunjukkan aktiviti antioksida yang tinggi di dalam asai DPPH penyah radikal, kuasa penurun dan logam pengkelat dalam aturan berikut (98.25%, 0.957, 81.97%) daripada ekstrak daun (90.04%, 0.652, 69.64%), batang (82.78%, 0.819, 36.70%) dan akar (63.25%, 0.469, 51.56%) Tabernaemontana corymbosa. Aktiviti antioksida yang tinggi di dalam ekstrak batang Tinospora crispa adalah disebabkan kehadiran apigenin dan magnoflorin. Aktiviti antioksida yang tinggi di dalam ekstrak Ervatamia coronaria adalah kerana kandungan fenol yang tinggi. Terdapat korelasi linear positif yang signifikan (r=0.788, p<0.001, r²=0.621) antara kandungan fenolik dan asai DPPH penyah radikal di dalam ekstrak mentah Tinospora crispa danTabernaemontana corymbosa. Sementara itu, korelasi positif sederhana yang signifikan ditunjukkan antara jumlah kandungan fenolik dan asai kuasa penurunan ferik (r=0.556, p< 0.05, r²=0.309). Walau bagaimanapun, tiada perbezaan yang signifikan dalam pekali kolerasi jumlah kandungan fenolik dan asai pengkelat logam.

 

Kata kunci: Aktiviti antioksida; Tabernaemontana corymbosa; jumlah kandungan fenolik; Tinospora crispa

 

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*Corresponding author; email: jamal@um.edu.my