Sains Malaysiana 43(8)(2014): 1149–1156

Biological Carbon Dioxide Sequestration Potential of Bacillus pumilus

(Potensi Pemencilan Biologi Karbon Dioksida oleh Bacillus pumilus)

T. KOMALA* & TAN. C. KHUN

Faculty of Engineering and Green Technology, Universiti Tunku Abdul Rahman, Perak Campus

Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia

Received: 9 August 2013/Accepted: 15 December 2013

ABSTRACT

Bacillus pumilis was isolated and identified from limestone and the ability towards carbon dioxide (CO₂) sequestration was demonstrated. B. pumilus (S₃ SC_1), isolated from Gua Tempurung, Gopeng, Perak was able to form calcite in the presence of calcium ions. B. pumilus was successfully characterized by using conventional biochemical characterization and 16s rDNA sequencing. Three types of experimental systems with B. pumilus, without B. pumilus and without continuous supply of CO₂ with the presence of B. pumilus which could produce extracellular carbonic were studied to determine the effects of bacterially produced carbonic anhydrase (CA) by B. pumilus in removing CO₂ as calcite. Through our current study, CO₂ sequestration ability of B. pumilus was proven.

Keywords: B. pumilus; carbon dioxide sequestration; carbonic anhydrase; characterization

ABSTRAK

Bacillus pumilis telah diasingkan dan dikenal pasti daripada batu kapur dan keupayaan ke arah pemencilan karbon dioksida (CO₂) telah dijalankan. B. pumilus (S3 SC_1) diasingkan dari Gua Tempurong, Gopeng, Perak mampu membentuk kalsit dengan kehadiran ion kalsium. B.pumilus berjaya dicirikan dengan menggunakan pencirian biokimia konvensional dan 16s rDNA. Tiga jenis sistem percubaan denganB. pumilus, tanpa B. pumilus dan tanpa bekalan berterusan CO₂ dengan kehadiranB. pumilus yang boleh menghasilkan ekstrasel carbonik telah dikaji untuk menentukan kesan bakteria hasilan karbonik anhidrase (CA) oleh B. pumilus dalam menghapuskan CO₂ sebagai kalsit. Melalui kajian ini, CO₂ keupayaan pemencilan oleh B. pumilus telah dibuktikan.

Kata kunci: B. pumilus; karbonik anhidrase; pemencilan karbon dioksida; pencirian

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*Corresponding author; email: komal_thiru@yahoo.com