Sains Malaysiana 43(9)(2014): 1345–1354

Characterisation and Stability of Pigments Extracted from Sargassum binderi

Obtained from Semporna, Sabah

(Pencirian dan Kestabilan Pigmen yang Diekstrak daripada Sargassum binderi dari Semporna, Sabah)

WU HON YIP, LIM SENG JOE, WAN AIDA WAN MUSTAPHA*, MOHAMAD YUSOF MASKAT & MAMOT SAID

School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

Received: 14 May 2013/Accepted: 31 December 2013

ABSTRACT

This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. Carotenoid and chlorophyll were found in the extract using UV-vis spectrophotometer (420 and 672 nm, respectively). Fucoxanthin was identified as the carotenoid present using HPLC, while its functional groups and structure were determined using FTIR and 1H NMR, respectively. The fucoxanthin-rich extract stability was tested on different pH (pH1-13), light exposure (dark and light) and storage temperature (4ºC, 25ºC and 50ºC). The stability tests showed that it was most stable at pH5-7, stored in dark condition and at the storage temperature of 4ºC and 25ºC. The fucoxanthin-rich extract from Sargassum binderi has potential to be applied as bioingredient and functional food as it is stable in normal storage conditions.

Keywords: Carotenoid; chlorophyll; fucoxanthin; pigment; Sargassum binderi

ABSTRAK

Kajian ini dijalankan untuk mengenal pasti pigmen yang diekstrak daripada rumpai laut perang Malaysia, Sargassum binderi dan juga kestabilannya dalam pelbagai keadaan. Pigmen diekstrak menggunakan metanol:kloroform:air (4:2:1, v/v/v), yang merupakan sebahagian daripada proses pengekstrakan fukoidan, dengan pigmen merupakan bahan buangan/sisa. Kehadiran karotenoid dan klorofil telah ditentukan dengan menggunakan spektrofotometer UV-vis (masing-masing 420 nm dan 672 nm). Fukoxantin telah dikenal pasti sebagai karotenoid yang hadir dengan menggunakan kromatografi cecair prestasi tinggi (KCPT), manakala kumpulan berfungsi dan struktur fukoxantin masing-masing dikenal pasti dengan menggunakan spektroskopi transformasi Fourier inframerah (FTIR) dan spektroskopi resonans magnet nukleus proton (1H NMR). Kestabilan pigmen telah diuji pada beberapa parameter yang berbeza, iaitu pH (pH1-13), pendedahan cahaya (cerah dan gelap) dan suhu simpanan (4ºC, 25ºC dan 50ºC). Ujian kestabilan menunjukkan bahawa pigmen adalah paling stabil pada pH5-7, disimpan dalam keadaan gelap dan pada suhu simpanan 4ºC dan 25ºC. Pigmen yang diekstrak daripada Sargassum binderi ini mempunyai potensi untuk diaplikasikan sebagai bioingredien dan makanan kerana ianya adalah stabil pada keadaan simpanan normal.

Kata kunci: Fukoxantin; karotenoid; klorofil; pigmen; Sargassum binderi

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*Corresponding author; email: wanaidawm@ukm.edu.my