Sains Malaysiana 45(2)(2016): 247–253

Petiole Vascular Bundles and its Taxonomic Value in the

Tribe Dipterocarpeae (Dipterocarpaceae)

(Berkas Vaskular Petiol dan Nilai Taksonominya dalam Tribus

Dipterocarpeae (Dipterocarpaceae))

T. Noraini¹, A.R. Ruzi¹, B.S. Ismail¹, B. Ummu Hani¹, S. Sahimi² & J. Azi Azeyanty²

¹Pusat Pengajian Sains Sekitaran dan Sumber Alam, Fakulti Sains dan Teknologi,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia

²School of Biological Sciences, University of Reading, RG2 2AS Berkshire, United Kingdom

Received: 8 August 2014/Accepted: 30 June 2015

ABSTRACT

A comprehensive survey and descriptions on the petiole anatomy was undertaken on 16 species belonging to four selected genera (Anisoptera, Cotylelobium, Vatica and Upuna) in the tribe Dipterocarpeae to investigate variations in vascular bundle structure. Methods used in this study were petiole sectioning using a sliding microtome, staining, mounting and observation under light microscope. Findings had shown that all species studied have complex vascular bundle structures, consisting of outer and medullary vascular bundles. Four different types of vascular bundle arrangements were found and described as Types 1, 2, 3 and 4. The vascular bundle types were determined based on the arrangement and system of vascular bundle strands present in the petiole transverse sections. These vascular bundle types were determined and suggested for easy reference. The results showed that vascular bundles can be used for identification of certain species and can definitely be used for classification of different genera. The presence of sclerenchyma cells is useful in differentiating genera. As a conclusion, the petiole vascular bundle anatomical characteristics have taxonomic value, especially in the identification of some species and possibly applicable in the classification of the tribe Dipterocarpeae.

Keywords: Anisoptera; Cotylelobium; Dipterocarpaceae; Dipterocarpeae; petiole anatomy; vascular bundles; Vatica

ABSTRAK

Kajian dan huraian komprehensif telah dijalankan ke atas anatomi petiol 16 spesies daripada empat genus terpilih (Anisoptera, Cotylelobium, Vatica dan Upuna) dalam tribus Dipterocarpeae untuk mengkaji variasi dalam struktur berkas vaskular. Kaedah yang digunakan dalam kajian ini ialah kaedah hirisan petiol menggunakan mikrotom gelongsor, pewarnaan, pelekapan dan pencerapan di bawah mikroskop cahaya. Hasil kajian menunjukkan semua spesies mempunyai berkas vaskular kompleks yang terdiri daripada berkas vaskular luar dan medulari. Empat jenis susunan berkas vaskular telah ditemui dan dihuraikan sebagai Jenis 1, 2, 3 dan 4. Jenis berkas vaskular ditentukan berdasarkan susunan dan sistem berkas vaskular dalam keratan rentas petiol. Jenis berkas vaskular ini ditentukan dan dicadangkan untuk memudahkan rujukan. Hasil kajian menunjukkan berkas vaskular boleh digunakan untuk pengecaman spesies dan terbukti boleh digunakan untuk pembezaan genus. Kehadiran kelompok sel sklerenkima juga berguna untuk membezakan genus. Kesimpulannya ciri anatomi berkas vaskular mempunyai nilai taksonomi terutamanya untuk pengecaman spesies dan berkemungkinan boleh diaplikasikan dalam pengelasan tribus Dipterocarpeae.

Kata kunci: Anatomy petiol; Anisoptera; berkas vaskular; Cotylelobium; Dipterocarpaceae; Dipterocarpeae; Vatica

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*Corresponding author; email: ntalip@ukm.edu.my