Sains Malaysiana 46(8)(2017): 1183–1189

http://dx.doi.org/10.17576/jsm-2017-4608-03

 

A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrumL.

(Protokol bagi Pembiakan Pantas dan Berfrekuensi Tinggi In Vitro Solanum nigrum L.)

 

LI-JUAN ZOU, JING-TIAN YANG & QING-GUI WU*

 

Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, 621000 Mianyang, China

 

Diserahkan: 24 Mei 2016/Diterima: 25 Januari 2017

 

ABSTRACT

Solanum nigrum L. is a species highly valued for its medicinal properties. In the present study, an efficient propagation system was established by using five explants of S. nigrum namely, roots, leaves, rooted hypocotyls, nodal segments and petioles. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for callus induction and organogenesis. Zeatin (ZT), thidiazuron (TDZ), kinetin (Kin) and indole-3-acetic acid (IAA) were found to induce multiple shoots. Shoot organogenesis was induced in the five explants. The highest mean for number of shoots per petioles (31.54±5.76) and rooted hypocotyls (44.00±1.51) with a 100% induction rate was obtained on Murashige and Skoog (MS) medium fortified with 0.4 mg/L IAA and 3.0 mg/L ZT. MS medium containing 0.4 mg/L IAA and 3.0 mg/L TDZ was found to be optimal for shoot regeneration of roots, leaves and nodal segments. The highest regeneration frequency (100%) with mean numbers of shoots equal to 38.77±6.87 for roots, 42.73±7.75 for leaves and 56.73±7.98 for nodal segments was produced. Regenerated shoots rooted effectively on half-strength MS medium and acclimatized successfully in soil with a 100% survival rate and normal growth. The protocol can be used for the large-scale propagation of S. nigrum to meet the increasing demand of commercial cultivation.

 

Keywords: Callus; explants; in vitro propagation; organogenesis; Solanum nigrum

 

 

ABSTRAK

Solanum nigrum L. ialah spesies yang amat dihargai bagi sifat perubatannya. Dalam kajian ini, sistem pembiakan cekap telah ditubuhkan dengan menggunakan lima eksplan daripada S. nigrum iaitu akar, daun, hipokotil berakar, nod segmen dan petiol. Pelbagai jenis pengatur pertumbuhan tumbuhan (PGRs) telah digunakan untuk menentukan gabungan hormon paling berkesan untuk aruhan kalus dan organogenesis. Zeatin (ZT), thidiazuron (TDZ) kinetin (Kin) dan asid indol-3-asetik (IAA) dilihat berjaya mengaruh beberapa pucuk. Pucuk organogenesis telah diaruh dalam lima eksplan. Min tertinggi bagi bilangan pucuk setiap petiol ialah (31.54±5.76) dan hipokotil berakar (44.00±1.51) dengan kadar aruhan 100% diperoleh pada medium Murashige dan Skoog (MS) dengan 0.4 mg/L IAA dan 3.0 mg/L ZT. Medium MS mengandungi 0.4 mg/L IAA dan 3.0 mg/L TDZ dilihat optimal untuk pertumbuhan semula pucuk untuk akar, daun dan nod segmen. Kekerapan penjanaan semula tertinggi (100%) dengan purata bilangan pucuk sama dengan 38.77±6.87 untuk akar, 42.73±7.75 daun dan 56.73±7.98 untuk nod segmen telah dihasilkan. Penghasilan semula pucuk berakar berkesan pada medium MS setengah-kekuatan dan berjaya mengaklimatisasikan dalam tanah dengan kadar kemandirian 100% dan pertumbuhan yang normal. Protokol ini boleh digunakan untuk pembiakan S. nigrum secara besar-besaran untuk memenuhi permintaan yang semakin meningkat dalam penanaman secara komersial.

 

Kata kunci: Eksplan; kalus; organogenesis; penanaman secarain vitro; Solanum nigrum

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*Pengarang untuk surat-menyurat; email: qgwu30@163.com