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Sains Malaysiana 46(9)(2017): 1465–1469
http://dx.doi.org/10.17576/jsm-2017-4609-15
Effect of Storage Time and Concentration of Used Cooking Oil on Polyhydroxyalkanoates (PHAs) Production by Cupriavidus necator H16 (Kesan
Masa Penyimpanan dan Kepekatan Minyak Masak Terpakai Terhadap Pengeluaran
Polihidroksyalkanoat
melalui Cupriavidus necator H16)
CHATSUDA KONGPENG1, JUTARUT IEWKITTAYAKORN1 & WILAIWAN CHOTIGEAT1,2*
1Department of Molecular
Biotechnology and Bioinformatics, Faculty of Science
Prince of Songkla
University, Hat Yai, Songkhla 90112, Thailand
2Center of Excellent for
Genomics & Bioinformatics Research, Faculty of Science, Prince of Songkla
University, Hat Yai, Songkhla 90112, Thailand
Received: 31 August
2016/Accepted: 17 January 2017
ABSTRACT
Polyhydroxyalkanoates (PHAs)
can be used to replace petrochemical plastics in many applications. However,
their production has limitation due to production cost. This research was
conducted using alternative carbon source from waste to synthesize PHAs
by Cupriavidus necator H16. In this study, PHAs
were produced from used cooking oil (UCO) and compared with
production PHAs from palm oil. Two UCO storage
times (4 and 10 weeks) and three UCO concentrations (10, 20 and
30 g/L) were used to determine the most optimum condition for PHA synthesis.
The best optimum condition for PHA synthesis was cultivated in
the medium containing 30 g/L of UCO at 4 weeks storage. The
cell dry weight (CDW) and PHA content
were 5.26±0.61 g/L and 27.36±2.04 wt. %, respectively. These results were
similar to cell cultivation using 20 g/L palm oil and 1% of fructose (5.93±0.33
g/L of CDW and 26.96±6.14 wt. % of PHA contents).
In addition, PHA content from the culture with 10 g/L of UCO stored
for 10 weeks was higher than PHA content from the culture
with 20 and 30 g/L of UCO. Thus, it can be concluded that UCO could
be used in PHA production.
Keywords: Bioplastics; Cupriavidus
necator H16; polyhydroxyalkanoates (PHAs); used cooking oil (UCO)
ABSTRAK
Polihidroksialkanoat (PHA)
boleh digunakan untuk menggantikan plastik petrokimia dalam kebanyakan
aplikasi. Walau bagaimanapun, pengeluarannya mempunyai had kerana kos
pengeluaran. Kajian ini dijalankan menggunakan sumber karbon alternatif
daripada bahan buangan untuk mensintesis PHA menggunakan Cupriavidus
necator H16. Dalam kajian ini, PHAs dihasilkan daripada minyak
masak terpakai (UCO) dan dibandingkan dengan
pengeluaran PHA daripada minyak sawit. Dua masa simpanan UCO (dua
4 dan 10 minggu) serta tiga kepekatan UCO (10, 20 dan 30 g/L) telah
digunakan untuk menentukan syarat keadaan optimum untuk sintesis PHA.
Keadaan optimum terbaik untuk sintesis PHA ialah dieram pada medium
yang mengandungi 30 g/L UCO pada 4 minggu penyimpanan. Berat
sel kering (CDW) dan kandungan PHA masing-masing adalah
5.26±0.61 g/L dan 27.36±2.04 % bt. Keputusan ini adalah sama dengan penanaman
sel menggunakan 20 g/L minyak sawit dan 1% fruktosa (5.93±0.33 g/L CDW dan
26.96±6.14 % bt. kandungan PHA). Di samping itu, kandungan PHA daripada
kultur dengan 10 g/L UCO yang disimpan selama 10 minggu
adalah lebih tinggi daripada kandungan PHA yang dikultur dengan 20 dan
30 g/L UCO. Oleh itu, dapat disimpulkan bahawa UCO boleh
digunakan dalam pengeluaran PHA.
Kata kunci: Bioplastik; minyak masak terpakai (UCO); Necator
cupriavidus H16; polihidroksialkanoat (PHA)
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*Corresponding author; email: wilaiwan58@hotmail.com
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