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http://dx.doi.org/10.17576/jsm-2018-4712-15
Sesquiterpene Production in Methyl
Jasmonate-Induced Persicaria minor Cell Suspension Culture
(Penghasilan Seskuiterpena dalam Kultur Ampaian
Sel Persicaria minor Diinduksi Metil Jasmonat)
PIRIYATASNI SELLAPAN, EMELDA ROSSELEENA ROHANI
& NORMAH MOHD NOOR*
Institute of Systems Biology, Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
Received: 30 May 2018/Accepted: 18 September
2018
ABSTRACT
Sesquiterpenes are a three-isoprene
unit compounds which belong to terpenoid family of secondary metabolites. These
volatile compounds are one of the major constituents of essential oils in
plants and plays major roles in plant signaling of defense mechanism. The
effects of methyl jasmonate (MeJA) concentrations (100 and 200 μM) on the
production of sesquiterpene compounds after incubation period for 1, 3, and 6
days were investigated in Persicaria minor cell suspension culture.
Headspace Solid-Phase Microextraction (HS-SPME) method was used to
absorb volatile compounds from suspension cells and liquid medium. They were
then analyzed through Gas Chromatography-Mass Spectrometry (GC-MS)
to identify sesquiterpene compounds. Among the 15 sesquiterpene compounds
identified, α- muurolene was found in significantly higher concentration
in all MeJA treated cultures. The results showed that α-muurolene was
detected in the suspension cells at the highest peak area of 14.17% at 100
μM MeJA treated cultures with 3-day incubation. Analysis of liquid medium
of the treatments identified secretion of α-muurolene into the culture
medium, with total peak area of 0.72%. These results showed that sesquiterpene
production in MeJA induced P. minor suspension culture depended on the
MeJA concentration and also culture incubation period.
Keywords: α-muurolene; cell
suspension culture; GC-MS; methyl jasmonate; sesquiterpene
ABSTRAK
Seskuiterpena adalah unit sebatian
tiga isoprena yang tergolong dalam keluarga terpenoid metabolit sekunder.
Sebatian meruap ini adalah juzuk utama dalam minyak pati daripada tumbuhan dan
memainkan peranan penting sebagai pengisyaratan bagi mekanisme pertahanan
tumbuhan. Kesan kepekatan metil jasmonat (MeJA) (100 dan 200 μM) ke atas penghasilan
seskuiterpena dengan tempoh masa pengeraman 1, 3 dan 6 hari telah dikaji dalam
kultur ampaian sel Persicaria minor. Pengekstrakan-mikro ruang kepala fasa (Headspace
Solid-Phase Microextraction) (HS-SPME) bersama kromatografi
gas-spektrometri jisim (GC-MS) digunakan untuk mengenal pasti
sebatian seskuiterpena daripada ekstrak ampaian sel. Daripada 15 sebatian
seskuiterpena yang dikenal pasti, α-muurolin didapati hadir pada kepekatan
yang signifikan bagi kesemua kultur yang diberi perlakuan MeJA. Keputusan
kajian menunjukkan α-muurolin dikesan pada luas puncak tertinggi 14.71%
dalam ampaian sel pada 100 μM MeJA dengan tiga hari pengeraman. Analisis
ke atas medium cecair mendapati kehadiran rembesan α-muurolin dengan luas
puncak keseluruhan 0.72%. Keputusan menunjukkan bahawa penghasilan
seskuiterpena dalam ampaian sel terinduksi MeJA bagi P. minor adalah
bergantung kepada kepekatan MeJA dan juga tempoh pengeraman.
Kata
kunci: α-muurolin; GC-MS; kultur ampaian sel; metil jasmonat;
seskuiterpena
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*Corresponding author; email: profnormah@yahoo.com
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