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Sains Malaysiana 47(1)(2018): 141–148
http://dx.doi.org/10.17576/jsm-2018-4701-17
STK15 Phe31Ile and Val57Ile Polymorphisms
Increase the Risk of Gastrointestinal Cancer
(Polimorfisme STK15 Phe31Ile dan Val57Ile Meningkatkan Risiko terhadap Kanser Gastrousus)
TIANXIN LAI1, ERIC TZYY JIANN CHONG1, JITT AUN CHUAH2, KEK HENG CHUA3 & PING-CHIN LEE1*
1Biotechnology Programme,
Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah Negeri di Bawah Bayu, Malaysia
2Surgery Department, Queen
Elizabeth Hospital, Jalan Penampang,
88200 Kota Kinabalu, Sabah Negeri di Bawah Bayu, Malaysia
3Department of Biomedical Science,
Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, Federal
Territory, Malaysia
Received: 15 January 2016/Accepted:
3 June 2017
ABSTRACT
STK15
is a serine/threonine kinase that regulates chromosomal segregation during
mitosis. Single nucleotide polymorphisms (SNPs)
in this gene, Phe31Ile (rs2273535) and Val57Ile (rs1047972), are inconsistently
associated with gastrointestinal cancer (GIC) across different
populations. However, this association is unclear in Malaysian population.
Therefore, this study investigated the association of STK15
Phe31Ile and Val57Ile polymorphisms to GIC risk in Malaysia. Genomic DNA was
extracted from 185 GIC patients and 1110 healthy controls
and was subjected to polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) analysis. SNPs
were further confirmed using sequencing. We found that the 31Phe allele and
31Phe/Phe genotype in the Phe31Ile SNP significantly
increased GIC risk in Malaysian population, particularly in gastric
cancer (p<0.017). The combined analysis for both SNPs
also increased the risk of GIC in this study. Etiological factors
such as age, gender and ethnicity were not associated with GIC in
the population. This is the first study to report the association of STK15
Phe31Ile and Val57Ile SNPs with an increased risk of GIC in
Malaysians; the 31Phe allele is exclusively associated with the risk of gastric
cancer. In addition, GIC incidences among Malaysians have
significantly shifted to a younger age (<50 years).
Keywords: Gastrointestinal
cancer; Malaysian population; STK15 polymorphisms
ABSTRAK
STK15
adalah kinase serin/treonina yang mengawal perpisahan kromosom semasa mitosis. Polimorfisme nukleotida
tunggal (SNPs) pada
gen ini, Phe31Ile (rs2273535) dan
Val57Ile (rs1047972) adalah dikaitkan
dengan kanser
gastrousus (GIC) secara
tidak tekal dalam populasi yang berbeza. Walau bagaimanapun, perkaitan
tersebut adalah
tidak jelas di dalam populasi di Malaysia.
Oleh
itu, penyelidikan ini mengkaji perkaitan
bagi polimorfisme
STK15
Phe31Ile dan Val57Ile terhadap
risiko GIC di Malaysia. DNA genom diekstrak
daripada 185 pesakit
GIC
dan 1110 kawalan yang sihat. Seterusnya, analisis tindak
balas berantai
polimerase pemotongan panjang cebisan (PCR-RFLP)
dijalankan dan
SNP
turut disahkan dengan menggunakan teknik penjujukan DNA.
Kami mendapati bahawa
alel 31Phe dan
genotip 31Phe/Phe dalam SNP Phe31Ile meningkatkan
risiko terhadap
GIC
dalam populasi di Malaysia secara signifikan, terutamanya dalam kanser gastrik (p<0.017). Analisis gabungan
bagi kedua-dua
SNP
juga meningkatkan risiko
terhadap GIC dalam
kajian ini.
Faktor etiologi seperti umur, jantina dan
etnik adalah
tidak berkait dengan
GIC
dalam populasi
ini. Kajian
ini merupakan kajian
pertama yang melaporkan
tentang perkaitan antara SNP STK15 Phe31Ile dan
Val57Ile dengan peningkatan
risiko terhadap
GIC
di Malaysia; terutamanya
alel 31Phe yang dikaitkan
dengan risiko
kanser gastrik. Selain itu, kejadian GIC dalam kalangan rakyat Malaysia telah beralih secara signifikan kepada usia yang lebih
muda (<50 tahun).
Kata kunci: Kanser gastrousus; polimorfisme STK15; populasi Malaysia
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*Corresponding author; email: leepc@ums.edu.my
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