Sains Malaysiana
47(2)(2018): 323-336
http://dx.doi.org/10.17576/jsm-2018-4702-15
Recent Advances in the
Use of Animal-Sourced Gelatine as Natural Polymers for Food, Cosmetics and
Pharmaceutical Applications
(Kemajuan Terkini dalam Penggunaan
Sumber Gelatin Haiwan sebagai Polimer Semula Jadi untuk Aplikasi Makanan,
Kosmetik dan Farmaseutik)
MOHD SHAKRIE
PALAN ABDULLAH1,2, MOHAMED IBRAHIM NOORDIN1, SYED IBRAHIM
MOHD ISMAIL3, NUR MURNISA MUSTAPHA4, MALINA JASAMAI5,
MOHD FAIRUZ DANIK6, WAN AZMAN WAN ISMAIL6 & AHMAD
FUAD SHAMSUDDIN6,7*
1Department of Pharmacy, Faculty of
Medicine, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia
2Pharmacy Enforcement Branch, Pharmacy
Services Division, Kelantan State Health Department, 16100 Kota Bharu, Kelantan
Darul Naim, Malaysia
3Ain Medicare Sdn. Bhd., Kawasan
Perindustrian Pengkalan Chepa 2, 16100 Kota Bharu, Kelantan Darul Naim,
Malaysia
4Department of Pharmacy, Raja Perempuan
Zainab II Hospital, 15100 Kota Bharu, Kelantan Darul Naim, Malaysia
5Drug and Herbal Research Centre, Faculty
of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala
Lumpur, Federal Territory, Malaysia
6Centre for Drug Delivery Research, Faculty
of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Federal Territory, Malaysia
7Faculty of Pharmacy and Health Sciences,
Universiti Kuala Lumpur, Royal College of Medicine Perak, (UniKL
RCMP), Jalan Greentown, 30450 Ipoh, Perak, Malaysia
Received: 11 May 2017/Accepted: 21 August
2017
ABSTRACT
Gelatine
is used as an excipient for various pharmaceutical dosage forms, such as
capsule shells (both hard and soft), tablets, suspensions, emulsions and
injections (e.g. plasma expanders). It is also broadly used in various
industries such as food and cosmetics. Gelatine is a biopolymer obtained from
discarded or unused materials of bovine, porcine, ovine, poultry and marine
industrial farms. The discarded materials can be the skin, tendons, cartilages,
bones and connective tissues. Gelatine sourced from animals is relatively easy
and inexpensive to produce. The potential needs of gelatine cannot be
overemphasised. Rising demands, health concerns and religious issues have
heightened the need for alternative sources of gelatine. This review presents
the various industrial uses of gelatine and the latest developments in
producing gelatine from various sources.
Keywords: Cosmetics;
food; gelatine, injections;
pharmaceuticals; plasma expanders
ABSTRAK
Gelatin diguna
sebagai eksipien (bahan tambahan) dalam pelbagai bentuk dos farmaseutik seperti
kelongsong kapsul (keras dan lembut), tablet, ampaian (suspensi), emulsi dan
suntikan (contoh: pengembang plasma). Ia juga diguna dalam pelbagai industri
lain seperti industri makanan dan kosmetik. Gelatin adalah biopolimer yang
diperoleh daripada bahan-bahan terbuang atau yang tidak diguna daripada haiwan
ternakan termasuk lembu, porsin, ovin, unggas dan ikan. Bahan-bahan terbuang
ini adalah seperti kulit, tendon, rawan, tulang dan tisu penghubung. Gelatin
daripada sumber haiwan adalah agak mudah dan murah untuk dihasilkan. Potensi
gelatin tidak dapat dinafikan. Permintaan yang semakin meningkat, masalah
kesihatan dan isu-isu agama telah meningkatkan keperluan untuk sumber
alternatif gelatin. Tinjauan ini membentangkan pelbagai kegunaan gelatin dalam
industri dan perkembangan terkini dalam penghasilan gelatin daripada pelbagai
sumber.
Kata kunci: Farmaseutik; gelatin; kosmetik; makanan; penambah plasma; sumber
alternatif
REFERENCES
Abdullah, M.S.P.,
Noordin, M.I., Nyamathulla, S., Ismail, S.I.M., Jasamai, M., Lam, K.W.,
Mustapha, N.M. & Shamsuddin, A.F. 2016. Physicochemical evaluation and spectroscopic
characterisation of gelatine from shank and toes of Gallus gallus domesticus. Sains Malaysiana 45(3): 435-449.
Alqudsi, S.G. 2014. Awareness and demand
for 100% Halal supply chain meat products. Procedia - Social and Behavioral
Sciences 130: 167-178.
Global Industry Analysts Inc. 2016. Gelatin Growing Applications in Food, Pharmaceutical and Nutritional Solutions
to Drive Demand for Gelatin.
http://www.strategyr.com/Marketresearch/Gelatin_Market_Trends.asp. Accessed on 3 May 2017.
Baldwin, E.A., Hagenmaier, R. & Bai, J.
2011. Edible Coatings and Films to
Improve Food Quality. Boca Raton: CRC Press.
Barry, F. 2014. Halal: Accessing the untapped
$30 Bn Pharma Market. Processing, CPhI worldwide Paris 2014, excipients,
raw materials and intermediates, processing equipment, APIs (active
pharmaceutical ingredients), delivery formulations, regulations.
http://www.in-pharmatechnologist.com/Processing/Halal-accessing-the-untapped-30bn-pharma-market?utm_source=copyright&utm_medium=OnSite&utm_campaign=copyright. Accessed on 12 December 2016.
Bhattacharyya, L., Schuber, S., Sheehan, C.
& William, R. 2006. Excipients: Background/Introduction. In Excipient Development for Pharmaceutical,
Biotechnology, and Drug Delivery Systems, edited by Katdare, A. &
Chaubal, M.V. New York: Informa Healthcare USA, Inc.
Brester, G. 2012. International Lamb Profile.
http://www.agmrc.org/commodities-products/livestock/lamb/international-lamb-profile/ Accessed on 1 April 2017.
Chowdhury, V.S., Sultana, H. & Furuse,
M. 2014. International perspectives on impacts of reproductive technologies for
world food production in Asia associated with poultry production. Adv. Exp. Med. Biol. 752: 229-237.
Dangi, A.A., Sheth, N.R., Sodha, H.H.,
Joshi, P.C., Bhalodiya, D.S., Panchal, A.C. & Ramanuj, P.R. 2011.
Formulation and development of vaccines and their selection for next generation. Bulletin of Pharmaceutical Research 1(3): 49-62.
Dunne, S., Shannon, B., Dunne, C. &
Cullen, W. 2013. A review of the differences and similarities between generic drugs
and their originator counterparts, including economic benefits associated with usage
of generic medicines, using Ireland as a case study. BMC
Pharmacology and Toxicology 14(1): 1-19.
Etxabide, A., Uranga, J., Guerrero, P.
& De La Caba, K. 2017. Development of active gelatin films by means of valorisation
of food processing waste: A review. Food
Hydrocolloids 68: 192-198.
Gahukar, R.T. 2011. Entomophagy and human food
security. International Journal of
Tropical Insect Science 31(3): 129-144.
Gómez-Guillén, M.C., Giménez, B.,
López-Caballero, M.E. & Montero, M.P. 2011. Functional and bioactive properties of collagen
and gelatin from alternative sources: A review. Food Hydrocolloids 25(8): 1813-1827.
Gorgieva, S. & Kokol, V. 2011. Collagen
vs. gelatine-based biomaterials and their biocompatibility: Review and perspectives.
In Biomaterials Applications for
Nanomedicine, edited by Pignatello,
R. Croatia: InTech.
Ha, T.L.B., Quan, T.M., Vu, D., Si, D. &
Andrades, J. 2013. Naturally derived biomaterials: Preparation and
application. In Regenerative
Medicine and Tissue Engineering,
edited by Andrades, J.A. Croatia: InTech. pp. 247-274.
Halloran, A., Roos, N., Eilenberg, J.,
Cerutti, A. & Bruun, S. 2016. Life cycle assessment of edible insects for food
protein: A review. Agronomy for
Sustainable Development 36(4): 57.
Hoque, M.E., Nuge, T., Yeow, T.K., Nordin,
N. & Prasad, R. 2015. Gelatin based scaffolds for tissue engineering - a review. Polymers Research Journal 9(1): 15-32.
Ige, O.O., Umoru, L.E. & Aribo, S.
2012. Natural products: A minefield of biomaterials. ISRN
Materials Science 2012: Article ID. 983062.
Intelligence, M. 2016. Global Pharmaceutical
Excipients Market Growth, Trends & Forecasts (2014-2020). Mordor
Intelligence. https://www.slideshare.net/alliedmarketresearch/excipient-market-opportunities-and-forecast-2014-2020.
Accessed on 12
November 2016.
Irwandi, J., Faridayanti, S., Mohamed,
E.S.M., Hamzah, M.S., Torla, H.H. & Che Man, Y.B. 2009. Extraction and characterization of gelatin from different marine fish species
in Malaysia. International Food Research Journal 16: 381-389.
Ivanova, E.P., Bazaka K. & Crawford,
R.J. 2014. Natural polymer biomaterials: Advanced applications. In New Functional Biomaterials for Medicine and
Healthcare, edited by Ivanova, E.P.,
Bazaka, K. & Crawford, R.J. Cambridge Univ Press, UK: Woodhead Publishing
Limited.
Jayaraman, K., Munira, H., Dababrata, C.
& Iranmanesh, M. 2013. The preference and consumption of chicken lovers
with race as a moderator-an empirical study in Malaysia. International Food Research Journal 20(1): 165-174.
Karim, A.A. & Bhat, R. 2008. Gelatin alternatives
for the food industry: Recent developments, challenges and prospects. Trends in Food Science & Technology 19(12): 644-656.
Karim, A.A. & Bhat, R. 2009. Fish gelatin:
Properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocolloids 23(3): 563-576.
Lasekan, A., Abu Bakar, F. & Hashim, D.
2013. Potential of chicken by-products as sources of useful biological resources. Waste Management 33(3): 552-565.
Li, Y., Lin, C., Wang, L., Liu, Y., Mu, X.,
Ma, Y. & Li, L. 2009. Maintenance of human embryonic stem cells on gelatin. Chinese Science Bulletin 54(22):
4214-4220.
Lira, A. & Pinsky, M.R. 2014. Choices
in fluid type and volume during resuscitation: Impact on patient outcomes. Annals of Intensive Care 4: 38-38.
Liu, M., Tan, H., Zhang, X., Liu, Z.,
Cheng, Y., Wang, D. & Wang, F. 2014. Hematopoietic effects and mechanisms of Fufang E'jiao Jiang on radiotherapy
and chemotherapy-induced myelosuppressed mice. Journal of Ethnopharmacology 152(3): 575-584.
Lopez-Cebral, R., Martin-Pastor, M.,
Parraga, J.E., Zorzi, G.K., Seijo, B. & Sanchez, A. 2011. Chemically modified gelatin as biomaterial
in the design of new nanomedicines. Medicinal Chemistry 7(3): 145-154.
Malafaya, P.B., Silva, G.A. & Reis,
R.L. 2007. Natural-origin polymers as carriers and scaffolds for biomolecules
and cell delivery in tissue engineering applications. Adv. Drug Deliv. Rev. 59(4-5): 207-233.
Mariod, A., Abdelwahab, S., Ibrahim, M.,
Mohan, S., Elgadir, M.A. & Ain, N. 2011. Preparation and characterization of gelatins from two Sudanese edible insects. Journal
of Food Science and Engineering 1(1): 45.
Mariod, A.A. & Adam, H.F. 2013. Review:
Gelatin, source, extraction and industrial applications. Acta Scientiarum Polonorum Technologia Alimentaria 12(2): 135-147.
Mariod, A.A. & Fadul, H. 2015.
Extraction and characterization of gelatin from two edible Sudanese insects and
its applications in ice cream making. Food
Science and Technology International 21(5): 380-391.
Markets, M.A. 2016.
Pharmaceutical Excipients Market by Type (Organic Chemical
(Sugar, Petrochemical, Glycerin), Inorganic Chemical), Functionality
(Filler, Binder, Lubricant, Preservative), Formulation (Oral, Topical,
Coating, Parenteral) - Global Forecast to 2021. Markets and Markets. https://www.marketsandmarkets.com/Market-Reports/pharma-excipients-market-956.html.
Accessed on 13 November 2016.
Markets, M.A. 2015. Drug
Delivery Technology Market by Route of Administration (Oral (Solid),
Pulmonary (Nebulizer), Injectable (Device), Ocular (Liquid), Nasal
(Drop), Topical (Solid), Implantable (Active), Transmucosal (Oral)),
End User (Hospital, Asc, Home Care) -Forecast to 2020. Pune, Maharashtra,
India. Accessed on 12 November 2016.
Mazaki, T., Shiozaki, Y., Yamane, K.,
Yoshida, A., Nakamura, M., Yoshida, Y., Zhou, D., Kitajima, T., Tanaka, M.,
Ito, Y., Ozaki, T. & Matsukawa, A. 2014. A novel, visible light-induced, rapidly cross-linkable gelatin scaffold
for osteochondral tissue engineering. Scientific
Reports 4: 4457.
Mohamad, A.A., Baharuddin, A.S. &
Ruskam, A. 2015. Halal industry in Singapore: A case study of nutraceutical products. Sains Humanika 4(2): 35-40.
Morrison, N. 2012a. Eu Animal Welfare Means
Pricier Gelatine: Rousselot.
http://www.in-pharmatechnologist.com/Ingredients/EU-animal-welfare-means-pricier-gelatine-Rousselot. Accessed on 25 May 2014.
Morrison, N. 2012b. Religion Plays Big Part
in Pharma Gelatine Shortage for India: Beroe.
http://www.in-pharmatechnologist.com/Ingredients/Religion-plays-big-part-in-pharma-gelatine-shortage-for-India-Beroe. Accessed on 25 May 2014.
Morrison, N. 2012c. Shortage of Indian Gelatin
Makers Could Be 'Tremendous' Opportunity for Foregin Firms, Says
Ipec.in-Pharmatechnologist.com. (16 July 2014): http://www.in-pharmatechnologist.com/Ingredients/Shortage-of-Indian-gelatin-makers-could-be-tremendous-opportunity-for-foregin-firms-says-IPEC. Accessed on 24 August 2014.
Mouriño, V., Cattalini, J.P., Roether,
J.A., Dubey, P., Roy, I. & Boccaccini, A.R. 2013. Composite polymer-bioceramic scaffolds with drug
delivery capability for bone tissue engineering. Expert Opinion on Drug Delivery 10(10): 1353-1365.
Murphy, C.M., O'brien, F.J., Little, D.G.
& Schindeler, A. 2013. Cell-scaffold interactions in the bone tissue engineering
triad. European Cells & Materials 26: 120-132.
Niazi, S.K. 2016. Biosimilars and Interchangeable Biologics: Strategic Elements. New
York: CRC Press.
Nongxuan, M.A.O. 2016. The anti-fatigue effect
of compound donkey-hide gelatine mucilage on free combat athletes. Journal of Computational and Theoretical
Nanoscience 13(3): 2091-2095.
Norimah, A.K., Jr., Safiah, M., Jamal, K.,
Haslinda, S., Zuhaida, H., Rohida, S., Fatimah, S., Norazlin, S., Poh, B.K.,
Kandiah, M., Zalilah, M.S., Wan Manan, W.M., Fatimah, S. & Azmi, M.Y. 2008.
Food consumption patterns: Findings from the Malaysian adult nutrition survey
(Mans). Malays J. Nutr. 14(1): 25-39.
Nur Hanani, Z.A., Roos, Y.H. & Kerry,
J.P. 2014. Use and application of gelatin as potential biodegradable packaging materials
for food products. International Journal
of Biological Macromolecules 71: 94-102.
Organization, W.H. 2015. The International Pharmacopoeia. Geneva:
World Health Organization.
Paguirigan, A. & Beebe, D.J. 2006.
Gelatin based microfluidic devices for cell culture. Lab Chip 6(3): 407-413.
Persidis, A. 1999. Tissue Engineering. Nature Biotechnology 17(5): 508-510.
Poultry, W. 2014. Malaysian Poultry Industry
Meeting Domestic Demand.World
Poultry. Meat:
http://www.worldpoultry.net/Meat/Articles/2014/3/Malaysian-poultry-industry-meeting-domestic-demand-1486503W/. Accessed on 30 March 2015.
Premalatha, M., Abbasi, T., Abbasi, T.
& Abbasi, S. A. 2011. Energy-efficient food production to reduce global
warming and ecodegradation: The use of edible insects. Renewable
and Sustainable Energy Reviews 15(9): 4357-4360.
Prugnaud, J.L. & Trouvin, J.H. 2012. Biosimilars: A New Generation of Biologics.
Paris: Springer.
Rafieian, F., Keramat, J. & Kadivar, M.
2013. Optimization of gelatin extraction from chicken deboner residue using RSM
method. Journal of Food Science and
Technology 50(2): 374-380.
Rastogi, V. & Samyn, P. 2015. Bio-based
coatings for paper applications. Coatings 5(4): 887.
Reports, A.M. 2016. World Pharmaceutical Excipients
Market - Opportunities and Forecasts, 2014 - 2020. Acute Market
Reports. Accessed on 3 May 2017.
Research, A.M. 2016a. World Excipient Market
- Opportunities and Forecast, 2014 - 2020.
https://www.researchandmarkets.com/reports/3641393/world-excipient-market-opportunities-and.
Accessed on 3 May
2017.
Research, G.V. 2016b. Gelatin Market Size
Expected to Reach $4.08 Billion by 2024.
Grand View Research, Inc. https://www.grandviewresearch.com/press-release/global-gelatin-market. Accessed on 5 May 2017.
Research, T.M. 2014. Collagen Peptide and
Gelatin Market (Source: Bovine, Porcine, Chicken, Marine and Others;
Application: Bone and Joint Health, Nutraceuticals, Cosmeceuticals,
Food and Beverage, Pet Food, Photography,Pharmaceuticals and Others)
- Global Industry Analysis, Size, Share, Growth, Trends & Forecast,
2014 - 2020. Transparency Market Research
(TMR). https://www.prnewswire.com/news-releases/collagen-peptide--gelatin-market-source-bovine-porcine-chicken-marine--others-application-bone-and-joint-health-nutraceuticals-cosmeceuticals-food--beverage-pet-food-photography-pharmaceuticals--others---global-i-300100047.html.
Accessed on 18 November 2016.
Research, T.M. 2015. Global Gelatin Market to Expand
at 6.75% cagr Because of Greater Application Scope in Various End-Use
Industries. Transparency
Market Research. https://www.transparencymarketresearch.com/pressrelease/gelatin.htm.
Accessed on 11
November 2016.
Roger, S.D. 2006. Biosimilars: How similar
or dissimilar are they? (Review Article). Nephrology 11(4): 341-346.
Rose, J.B., Pacelli, S., Haj, A.J.E., Dua,
H.S., Hopkinson, A., White, L.J. & Rose, F.R.a.J. 2014. Gelatin-based materials in ocular tissue
engineering. Materials 7(4):
3106-3135.
Rottensteiner, U., Sarker, B., Heusinger,
D., Dafinova, D., Rath, S.N., Beier, J.P., Kneser, U., Horch, R.E., Detsch, R.,
Boccaccini, A.R. & Arkudas, A. 2014. in
vitro and in vivo biocompatibility
of alginate dialdehyde/gelatin hydrogels with and without nanoscaled bioactive glass
for bone tissue engineering applications. Materials
(1996-1944) 7(3): 1957-1974.
Rumpold, B.A. & Schlüter, O.K. 2013.
Potential and challenges of insects as an innovative source for food and feed production. Innovative Food Science & Emerging
Technologies 17: 1-11.
Rustad, T., Storro, I. & Slizyte, R.
2011. Possibilities for the utilisation of marine by‐products. International
Journal of Food Science & Technology 46(10): 2001-2014.
Sahilah, A.M., Fadly, L., Norrakiah, A.S.,
Aminah, A., Wan Aida, W.M., Ma'aruf, A.G. & Khan, A. 2012. Halal market surveillance
of soft and hard gel capsules in pharmaceutical products using PCR and southern-hybridization
on the biochip analysis. International
Food Research Journal 19(1): 371-375.
Sahoo, N., Sahoo, R.K., Biswas, N., Guha,
A. & Kuotsu, K. 2015. Recent advancement of gelatin nanoparticles in drug
and vaccine delivery. International
Journal of Biological Macromolecules 81: 317-331.
Saw, M.M., Chandler, B. & Ho, K.M.
2012. Benefits and risks of using gelatin solution as a plasma expander for perioperative
and critically ill patients: A meta-analysis. Anaesth Intensive Care 40(1): 17-32.
Schrieber, R. & Gareis, H. 2007. Gelatine Handbook: Theory and Industrial
Practice. Weinheim: Wiley-VCH Verlag GmbH & Co.
Sekhon, B.S. & Saluja, V. 2011.
Biosimilars: An Overview. Biosimilars 1(1): 1-11.
Shah, H. & Yusof, F. 2014. Gelatin as
an ingredient in food and pharmaceutical products: An Islamic perspective. Advances in Environmental Biology 8(3):
774-780.
Silva, S.S., Mano, J.F. & Reis, R.L.
2010. Potential applications of natural origin polymer-based systems in soft tissue
regeneration. Critical Reviews in Biotechnology 30(3): 200-221.
Site, T.P. 2015. Global Poultry Trends - Asia
Accounts for a Third of Chicken Meat Output. http://www.thepoultrysite.com/articles/3533/global-poultry-trends-asia-accounts-for-a-third-of-chicken-meat-output/ Accessed on 30 September 2016.
Songchotikunpan, P., Tattiyakul, J. &
Supaphol, P. 2008. Extraction and electrospinning of gelatin from fish skin. International Journal of Biological
Macromolecules 42(3): 247-255.
Su, K. & Wang, C. 2015. Recent advances
in the use of gelatin in biomedical research. Biotechnol. Lett. 37(11): 2139-2145.
Thassu, D., Deleers, M. & Pathak, Y.
2007. Drugs and the Pharmaceutical
Science: Nanoparticulate Drug Delivery Systems. New York: Informa
Healthcare Inc.
Totre, J., Ickowicz, D. & Domb, A.J.
2011. Properties and hemostatic application of gelatin. In Biodegradable
Polymers in Clinical Use and Clinical Development, edited by Domb, A.J., Kumar, N. & Ezra,
A. New York: John Wiley & Sons, Inc. pp. 91-109.
Varela, P. & Fiszman, S.M. 2013.
Exploring consumers' knowledge and perceptions of hydrocolloids used as food additives
and ingredients. Food Hydrocolloids 30(1): 477-484.
Walker, N. 2015. Excipients Market Growing
but Novel Technologies Needed. American
Pharmaceutical Review. http://www.americanpharmaceuticalreview.com/Featured-Articles/181887-Excipients-Market-Growing-But-Novel-Technologies-Needed/ Accessed on 5 January 2016.
Wang, L., An, X., Yang, F., Xin, Z., Zhao,
L. & Hu, Q. 2008. Isolation and characterisation of collagens from the skin,
scale and bone of deep-sea redfish (Sebastes
mentella). Food Chemistry 108(2): 616-623.
Wang, S. 2011. Patent search on biologics
as potential biosimilar candidates. World
Patent Information 33(1): 67-71.
Wray, L.S. & Kaplan, D.L. 2014.
Biomaterials for scaffolds: Natural polymers. In Scaffolds
for Tissue Engineering: Biological Design, Materials, and Fabrication, edited by Migliaresi, C. & Antonella, M. Singapore:
Pan Stanford Publishing Pte. Ltd. p. 301.
ShanghaiDaily.com 2016. Counterfeit Donkey Hides Flood TCM Market.
September 28, 2016. https://www.shine.cn/archive/national/Counterfeit-donkey-hides-flood-TCM-market/shdaily.shtml.
Accessed on 5 January 2017.
Yadav, P., Yadav, H., Shah, V.G., Shah, G.
& Dhaka, G. 2015. Biomedical biopolymers, their origin and evolution in biomedical
sciences: A systematic review. Journal of
Clinical and Diagnostic Research (JCDR) 9(9): ZE21-ZE25.
Zarychanski, R., Abou-Setta, A.M., Turgeon,
A.F., Houston, B.L., Mcintyre, L., Marshall, J.C. & Fergusson, D.A. 2013.
Association of hydroxyethyl starch administration with mortality and acute kidney
injury in critically ill patients requiring volume resuscitation: A systematic review
and meta-analysis. JAMA 309(7):
678-688.
Zhang, G., Yue, X., Fan, A. & Liu, G.
2010. Reutilization of waste chicken bone as nutrients source. 4th
International Conference on Bioinformatics and Biomedical Engineering. pp. 1-4. http://dx.doi.org/10.1109%2FICBBE.2010.5517934.
Zhao, Y., Li, Z., Yang, W., Xue, C., Wang,
Y., Dong, J. & Xue, Y. 2016. Modification of gelatine with Galla chinensis extract, a natural crosslinker. International
Journal of Food Properties 19(4): 731-744.
Zhu, J. & Marchant, R.E. 2011. Design properties
of hydrogel tissue-engineering scaffolds. Expert Review of Medical Devices 8(5): 607-626.
*Corresponding author; email: fuad.shamsuddin@unikl.edu.my
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