Sains Malaysiana 50(2)(2021): 279-286

http://dx.doi.org/10.17576/jsm-2021-5002-01

 

Identification and Physiological Characteristics of Potential Indigenous Bacteria as Bioremediation Agent in the Wastewater of Sugar Factory

 (Pengecaman dan Pencirian Fisiologi Potensi Bakteria Asli sebagai Agen Bio-Pemulihan dalam AirSisa Kilang Gula)

 

MIKCHAELL ALFANOV PARDAMEAN¹, R ADHARYAN ISLAMY², VERYL HASAN³*, ENDANG YULI HERAWATI⁴ & NURUL MUTMAINNAH⁴

 

¹Department of Fisheries and Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Malang City, East Java 65145, Indonesia

 

²Department of Aquaculture, Faculty of Fisheries and Marine Science, Brawijaya University, Malang City, East Java 65145, Indonesia

 

³Department of Fish Health Management and Aquaculture, Fisheries and Marine Faculty, Airlangga University, Surabaya, East Java, Indonesia

⁴Departement of Water Resources Management, Faculty of Fisheries and Marine Science, Brawijaya University, Malang City, East Java 65145, Indonesia

 

Received: 19 April 2020/Accepted: 7 July 2020

 

ABSTRACT

Wastewater is the remainder of an activity released in the liquid form. Wastewater product is feared to have negative influence on the environmental balance; therefore, it is necessary to measure the quality standards of wastewater as a reference in the disposal and treatment. Bioremediation is an environmentally friendly technology utilizing microorganisms as agents in the process of cleaning or restoring wastewater conditions. The use of microorganism services can reduce the concentration of organic waste into simpler organic compounds by converting organic compounds into CO₂, CH₄, H₂, and H₂S, as well as water and energy intended for the process of growth and production of microorganisms in the remediation process. This study aims to identify the type of dominant bacteria growing in the wastewater of the sugar factory and has a potential role as a bioremediation agent for the waste. The method used in this study is the experimental, by observing several environmental parameters as indicators, among others, BOD, COD, TSS, and pH. The results of this study found two types of dominant bacteria, i.e., Staphylococcus aureus and Bacillus subtilis, then used as bioremediation agents. The bioremediation activity was able to reduce pH, BOD, and COD levels.

 

Keywords: Bacillus subtilis; bioremediation; indigenous bacteria; Staphylococcus aureus; wastewater

 

ABSTRAK

Air sisa adalah sisa aktiviti yang dikeluarkan dalam bentuk cecair. Produk air buangan dikhuatiri mempunyai pengaruh negatif terhadap keseimbangan alam sekitar; oleh itu, adalah penting untuk mengetahui piawai kualiti air sisa sebagai rujukan dalam pembuangan dan rawatan. Biopemulihan adalah teknologi mesra alam yang memanfaatkan mikroorganisma sebagai agen dalam proses pembersihan atau pemulihan keadaan air sisa. Penggunaan perkhidmatan mikroorganisma dapat mengurangkan kepekatan sisa organik menjadi sebatian organik yang lebih sederhana dengan mengubah sebatian organik menjadi CO₂, CH₄, H₂ dan H₂S, serta air dan tenaga yang dimaksudkan untuk proses pertumbuhan dan pengeluaran mikroorganisma dalam proses pemulihan. Kajian ini bertujuan untuk mengenal pasti jenis bakteria dominan yang tumbuh di dalam air sisa kilang gula dan berpotensi berperanan sebagai agen biopemulihan bagi sisa tersebut. Kaedah yang digunakan dalam kajian ini adalah kaedah uji kaji dengan memerhatikan beberapa parameter persekitaran sebagai petunjuk, antara lain BOD, COD, TSS dan pH. Hasil kajian ini mendapati dua jenis bakteria dominan, iaituStaphylococcus aureus dan Bacillus subtilis, kemudian digunakan sebagai agen biopemulihan. Aktiviti biopemulihan dapat mengurangkan tahap pH, BOD dan COD.

 

Kata kunci: Air sisa; Bacillus subtilis; biopemulihan; bakteria asli; Staphylococcus aureus

 

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*Corresponding author; email: veryl.hasan@fpk.unair.ac.id