Sains Malaysiana 50(9)(2021): 2663-2674

http://doi.org/10.17576/jsm-2021-5009-14

Alkaloid Caulerpin and Cytotoxic Activity against NCL-H460 Lung Cancer Cells Isolated along with β-sitosterol from the Halimeda cylindracea Decaisne

(Alkaloid Kaulerpin dan Aktiviti Kesitotoksikannya terhadapSel Barah Paru-Paru NCL-H460

Dipencilkan bersama β-sitosterol daripada Halimeda cylindracea Decaisne)

IWAN DINI¹, NUNUK HARIANI SOEKAMTO²*, FIRDAUS², UNANG SUPRATMAN^3,4 & JALIFAH LATIP⁵

¹Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia

²Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Hasanuddin, Makassar, Indonesia

³Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor, Indonesia

⁴Central Laboratory of Universitas Padjadjaran, Jatinangor, Indonesia

⁵School of Chemical Science and Food Technology, Faculty of Science and Tecnology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 5 May 2020/Accepted: 19 January 2021

ABSTRACT

Alkaloid caulerpin (1), along with β-sitosterol (2), were isolated from the n-hexane extract of the macroalga Halimeda cylindracea Decaisne. The chemical structure was identified by a spectroscopic method including IR, MS, UV, NMR 1D, NMR 2D, and comparison with data of spectra previously reported. Compounds (1) and (2) were isolated for the first time from this macroalga. Compund (1) were evaluated for their cytotoxicity activity against NCL-H460 lung cancer cells in vitro and showed moderate activity with IC₅₀ value of 20.05 µg/mL.

Keywords: β-sitosterol; caulerpin; Halimeda cylindracea; NCL-H460 lung cancer cells

ABSTRAK

Alkaloid kaulerpin (1), bersama dengan β-sitosterol (2), diasingkan daripada ekstrak n-heksana daripada makroalga Halimeda cylindracea Decaisne. Struktur kimia dikenal pasti dengan kaedah spektroskopi termasuk IR, MS, UV, NMR 1D, NMR 2D dan perbandingan dengan data spektrum yang dilaporkan sebelumnya. Sebatian (1) dan (2) diasingkan untuk pertama kalinya daripada makroalga ini. Sebatian (1) dinilai untuk aktiviti kesitotoksikannya terhadap sel barah paru-paru NCL-H460 secara in vitro dan menunjukkan aktiviti sederhana dengan nilai IC₅₀ 20.05 µg/mL.

Kata kunci: β-sitosterol; Halimeda cylindracea; kaulerpin; sel barah paru-paru NCL-H460

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*Corresponding author; email: nunukhariani@unhas.ac.id