Sains Malaysiana 51(7)(2022): 2073-2085

http://doi.org/10.17576/jsm-2022-5107-11

Survivability and Release Process of Lactobacillus plantarum SU-LS36 Encapsulated with Native and Modified Taro Starch Under Simulated Digestive Conditions

(Kemandirian dan Proses Pelepasan Pengkapsulan Lactobacillus plantarum SU-LS36 dengan Kanji Taro Asli dan Terubah Suai dalam Keadaan Simulasi Pencernaan)

R. HARYO BIMO SETIARTO^1,2,*, HARSI DEWANTARI KUSUMANINGRUM¹, BETTY SRI LAKSMI JENIE¹, TATIK KHUSNIATI², MASRUKHIN³ & SULISTIANI²

¹Department of Food Science and Technology, Faculty of Agricultural Technology and Engineering, IPB University, Dramaga Bogor, 16680 West Java, Indonesia

²Research Center for Applied Biology, National Research, and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km 46, Cibinong Science Center, Cibinong, Bogor, 16911 West Java, Indonesia

³Research Center for Biosystematics and Evolution, National Research, and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km 46, Cibinong Science Center, Cibinong, Bogor, 16911 West Java, Indonesia

Received: 10 July 2021/Accepted: 9 December 2021

ABSTRACT

This research aimed to evaluate the viability, survivability, and release process of the encapsulated Lactobacillus plantarum SU-LS36 in the simulated gastric juice (SGJ), simulated intestinal juice (SIJ), and simulated colon juice (SCJ). We tested four types of encapsulations: native taro starch (NTS), modified taro starch (MTS) by heat moisture treatment (HMT), autoclaving-cooling-2 cycles (AC-2C), and maltodextrin (commercial encapsulant). We found that L. plantarum SU-LS36 with AC-2C-modified taro starch (MTS) showed the highest viability in SGJ (6.95 log CFU/g), SIJ (7.09 log CFU/g), and SCJ (7.85 log CFU/g) after incubation up to 4 h. AC-2C MTS dissolved or released more rapidly from its encapsulant material in the colon in SCJ than in NTS, HMT MTS, and maltodextrin. The longest time release of L. plantarum SU-LS36 encapsulated in AC-2C MTS was 3 h in SIJ conditions, 2 h in SGJ, and the fastest (1 h) in SCJ. The encapsulated L. plantarum SU-LS36 was released through a dissolution process (SGJ and SCJ) and by pancreatin activity (SIJ). Conclusively, AC-2C MTS could maintain the viability of L. plantarum SU-LS36 cells to the colon at 6.04 log CFU/g and fulfilled the minimum requirement of biovalue (MBV) probiotics set forth by the US FDA (6-7 log CFU/g).

Keywords: Encapsulation; Lactobacillus plantarum SU-LS36; simulated digestion in-vitro; survivability; taro starch 

ABSTRAK

Penyelidikan ini bertujuan untuk menilai kebolehhidupan, kemandirian dan proses pembebasan pengkapsulan Lactobacillus plantarum SU-LS36 dalam jus gaster simulasi (SGJ), jus usus simulasi (SIJ) dan jus kolon simulasi (SCJ). Kami menguji empat jenis pengkapsulan: kanji ubi keladi asli (NTS), kanji ubi keladi terubah suai (MTS) dengan rawatan kelembapan haba (HMT), kitaran penyejukan-2 autoklaf (AC-2C) dan maltodekstrin (pengkapsulan komersial). Kami mendapati bahawa L. plantarum SU-LS36 dengan kanji ubi keladi (MTS) diubah suai AC-2C telah menunjukkan kebolehhidupan yang tertinggi dalam SGJ (6.95 log CFU/g), SIJ (7.09 log CFU/g) dan SCJ (7.85 log CFU/g) selepas pengeraman sehingga 4 jam. AC-2C MTS larut atau dibebaskan dengan lebih cepat daripada bahan pengkapsulan dalam kolon di SCJ berbanding NTS, HMT MTS dan maltodekstrin. Pelepasan masa terpanjang L. plantarum SU-LS36 yang terkandung dalam AC-2C MTS ialah 3 jam dalam keadaan SIJ, 2 jam dalam SGJ, dan terpantas (1 jam) dalam SCJ. L. plantarum SU-LS36 terkapsul telah dilepaskan melalui proses pelarutan (SGJ dan SCJ) dan oleh aktiviti pancreatin (SIJ). Secara kesimpulannya, AC-2C MTS boleh mengekalkan kebolehhidupan sel L. plantarum SU-LS36 ke kolon pada 6.04 log CFU/g dan memenuhi keperluan minimum nilai bio probiotik (MBV) yang ditetapkan oleh FDA AS (6-7 log CFU/g).

Kata kunci: Kanji ubi keladi; kemandirian; Lactobacillus plantarum SU-LS36; pengkapsulan; simulasi pencernaan in-vitro

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^*Corresponding author; email: haryobimo88@gmail.com