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http://doi.org/10.17576/jsm-2022-5108-01
Transient or Permanent Inhabitants: Cultured Internal Microbiota of Cichlidogyrus thurstonae and Scutogyrus longicornis (Monogenea: Ancyrocephalidae) from Oreochromis sp.
(Penghuni Sementara atau Kekal: Mikrobiota Dalaman Biakan Cichlidogyrus thurstonae dan Scutogyrus longicornis (Monogenea: Ancyrocephalidae) daripada Oreochromis sp.)
ZHI-ZHANG
ONG1, YEAK-HENG CHONG1 & WEY-LIM WONG1,2,*
1Department of Biological Science, Faculty of
Science, Universiti Tunku Abdul Rahman, Jalan Universiti,
Bandar Barat, 31900 Kampar, Perak Darul Ridzuan, Malaysia
2Centre for Agriculture and Food Research, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900
Kampar, Perak Darul Ridzuan,
Malaysia
Received: 4 August 2021/Accepted: 15 February 2022
Abstract
Monogeneans,
a class of parasitic platyhelminthes, are usually found on the surface of fish.
When it feeds on its host, it harbours bacteria,
which can survive in its gut. Occasionally, the monogeneans may cause damages
on fish tissue while feeding and may allow secondary infection. The present
study aimed to isolate and identify culturable bacteria obtained within the gill monogeneans, Cichlidogyrus thurstonaeand Scutogyrus longicornis, and gill surface of the
tilapia fish, Oreochromis sp. based on 16S ribosomal RNA gene
sequencing. Monogeneans were isolated from the fish gill filaments and surface
disinfected using 70% ethanol before squashed aseptically on Luria Bertani (LB) agar to isolate the internal microbiota. A
total of five bacteria species, namely Burkholderia sp., Enterobacter hormaechei, Enterobacter sp., Ochrobactrum intermedium and Pantoea sp., were found within C. thurstonae, whilst a total of eight bacteria
species, namely Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium, Acinetobacter bereziniae, Escherichia hermannii, and Staphylococcus saprophyticus,
were found within S. longicornis in which B. contaminans, P. dispersa, and S. yabuuchiaewere
also found on the surface of the tilapia fish gill filaments. Enterobacter bugandensisand Acinetobacter pittiiwere found solely on the surface of the
gill filaments. These bacteria are also found in the
environment and some of them are believed to be pathogenic to fish. We suggest that
monogeneans may serve as potential bacteria reservoirs, which facilitate the
transmission of bacteria.
Keywords: Bacteria; identification; internal microbiota; molecular; monogenean; tilapia
Abstrak
Monogenea ialah salah satu kelas parasit platyhelminthes yang sering ditemui pada permukaan badan ikan. Apabila monogenea memakan tisu hos ikan, ia memperoleh bakteria
yang dapat hidup di dalam usus monogenea. Kadangkala, monogenea boleh menyebabkan kecederaan pada tisu ikan semasa makan yang mungkin menyebabkan jangkitan
sekunder pada ikan. Kajian ini bertujuan memencilkan dan mengenal pasti bakteria yang boleh dikultur daripada monogenea, Cichlidogyrus thurstonae dan Scutogyrus
longicornis serta pada permukaan insang ikan tilapia, Oreochromis sp. berdasarkan jujukan gen 16S ribosomal RNA. Monogenea dipencilkan daripada filamen insang ikan
dan permukaan dibilas dengan 70% etanol sebelum dilenyek secara aseptik pada agar LB untuk pemencilan mikrobiota dalaman. Sebanyak lima spesies bakteria, iaitu Burkholderia sp., Enterobacter hormaechei, Enterobacter sp., Ochrobactrum intermedium dan Pantoea sp., dijumpai di dalam C. thurstonae manakala sejumlah lapan
spesies bakteria, iaitu Burkholderia contaminans, Pantoea dispersa, Sphingomonas yabuuchiae, Rhizobium pusense, O. intermedium, Acinetobacter bereziniae, Escherichia hermannii dan Staphylococcus saprophyticus dijumpai pada S. longicornis dengan B. contaminans, P. dispersadan S. yabuuchiaejuga dijumpai
pada permukaan filamen insang ikan. Walau bagaimanapun, Enterobacter bugandensisdan Acinetobacter pittiihanya dijumpai pada permukaan filamen insang
sahaja. Bakteria ini juga boleh didapati daripada persekitaran dan sebahagian daripada bakteria ini dipercayai bersifat patogen kepada ikan. Kami mendapati
monogenea berkemungkinan berfungsi sebagai perumah yang memudahkan penularan bakteria.
Kata kunci: Bakteria; mikrobiota dalaman; molekul; monogenea; pengenalpastian; tilapia
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*Corresponding author; email: wongwl@utar.edu.my
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