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Sains Malaysiana 51(8)(2022):
http://doi.org/10.17576/jsm-2022-5108-06
Hot Water
Extract from Saccharomyces cerevisiae Scavenges DPPH and Reduces Senescence Associated b-Galactosidase (Sa-b-Gal) in Human Dermal Fibroblasts
(Ekstrak Air Panas daripada Saccharomyces cerevisiae Menghimpun
DPPH dan Mengurangkan b-Galaktosidase (Sa-b-Gal) Berkait Senesensdalam Fibroblas Dermal Manusia)
KHAIZURIN TAJUL ARIFIN*, NOOR IKHWAN
SHANSUDDIN, NORWAHIDAH ABDUL KARIM & SUZANA MAKPOL
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia
Medical Centre, 56000 Kuala Lumpur, Federal Territory, Malaysia
Received: 27 August 2020/Accepted: 25 February 2022
Abstract
Extracts from Saccharomyces cerevisiae are incorporated
in a lot of cosmetic products on the
market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent
activity of an extract from S. cerevisiae on an in vitro model,
the human dermal fibroblast (HDF) cell culture. We chronicled the development
of the extraction method and the subsequent biochemical assays. We used two
extraction methods which were hot water extraction and rapid spin. The optimum
duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay,
which also proved that the extracts exhibited antioxidant activity. Hot water extract showed a higher antioxidant
activity, and not toxic to HDF. When subjected to senescence-associated
β-galactosidase (SA-β-Gal) assay, the hot water extract significantly
reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 < population doubling <
40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot water extract possessed antioxidant activity by
scavenging DPPH, and anti-senescent activity by reducing the expression of
SA-β-Gal in pre-senescent and senescent HDF.
Keywords: Ageing; DPPH; Saccharomyces cerevisiae;
senescence
Abstrak
Ekstrak daripada Saccharomyces cerevisiae digabungkan dalam banyak produk kosmetik di pasaran. Ia dikatakan mempunyai kesan yang baik pada kulit manusia, tetapi masih kurang laporan saintifik tentang keberkesanan ekstraknya. Kami telah menguji kesan ketoksikan dan aktiviti anti-penuaan bagi ekstrak mentah daripada S.
cerevisiae pada model in vitro,
kultur sel fibroblas dermal manusia (HDF). Kami melaporkan pembangunan kaedah pengekstrakan dan asai biokimia seterusnya. Kami menggunakan dua kaedah pengekstrakan iaitu pengekstrakan air panas dan putaran cepat. Tempoh dan fasa pertumbuhan optimum untuk menuai S. cerevisiae ditentukan dengan asai 2,2-difenil-1-pikrilhidrazil (DPPH), yang juga membuktikan bahawa ekstrak mentah menunjukkan aktiviti antioksidan. Ekstrak air panas menunjukkan aktiviti antioksidan yang lebih tinggi dan tidak toksik kepada HDF. Apabila dilakukan asai β-galaktosidase berkait penuaan (SA-β-Gal), ekstrak air panas mengurangkan ekspresi SA-β-Gal dalam HDF pra-senesens (pasaj 20, 30 < populasi berganda < 40) dan senesens (pasaj 30, populasi berganda > 50). Kesimpulannya, ekstrak air panas S. cerevisiae mempunyai aktiviti antioksidan dengan menghimpun DPPH dan aktiviti anti-senesens dengan mengurangkan ekspresi SA-β-Gal dalam HDF pra-senesens dan senesens.
Kata kunci: DPPH; penuaan; Saccharomyces cerevisiae;
senses
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*Corresponding author; email: khaizurin.tajul.arifin@ppukm.ukm.edu.my
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