Sains Malaysiana 47(3)(2018): 499–509

http://dx.doi.org/10.17576/jsm-2018-4703-09

Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana

(Pengekspresan Protein Glikoprotein Virus Nipah secara Transien dalam Nicotiana benthamiana)

GAN KHAI SWAN, TAN CHON SENG, ROFINA YASMIN OTHMAN & JENNIFER ANN HARIKRISHNA*

Centre for Research in Biotechnology for Agriculture, University of Malaya, 50603, Kuala Lumpur, Malaysia

Diserahkan: 23 Mac 2017/Diterima: 13 Oktober 2017

ABSTRACT

The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.

Keywords: Biotechnology; KDEL; plants; tobacco PR1a; vaccine

ABSTRAK

Virus Nipah adalah virus yang sangat virulen bagi ternakan khinzir dan manusia. Pelekat sampul sel gliko protein G virus Nipah memainkan peranan penting dalam kemasukan virus dan peneutralan antibodi di dalam sel perumah mamalia. Oleh itu, ia dianggap sebagai satu calon yang baik untuk dijadikan vaksin. Memandangkan sistem asai transien dalam tumbuhan semakin diberi perhatian sebagai cara alternatif penghasilan vaksin, protein G virus Nipah telah diekspres dalam tumbuhan Nicotiana benthamiana menerusi kaedah agroinfiltrasi di dalam kajian ini. Dalam kajian ini, pengekspresan RNA dan protein G dalam Nicotiana benthamiana mencapai tahap maksimum pada hari ke-9 selepas infiltrasi. Protein G yang ditulenkan menunjukkan tindak balas yang khusus dengan serum arnab anti-Nipah virus dalam analisis pedapan Western. Penemuan ini menunjukkan protein rekombinan G virus Nipah berpotensi dijadikan vaksin.

Kata kunci: Bioteknologi; KDEL; tembakau PR1-a; tumbuhan; vaksin

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*Pengarang untuk surat-menyurat; email: jennihari@um.edu.my