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Sains Malaysiana 49(9)(2020): 2035-2042
http://dx.doi.org/10.17576/jsm-2020-4909-01
Cultivation
of Chlorella vulgaris in
Anaerobically Digested Dairy Manure Wastewater (ADDMW) for Protein and
Chlorophyll Production
(Pengkulturan Chlorella
vulgaris dalam Air Buangan Sapi Cernaan Anaerobik (ADDMW) bagi Penghasilan Protein dan Klorofil)
TAUFIK
TAUFIKURAHMAN1*, MUHAMMAD ARIEF ARDIANSYAH1, NOVI TRI
ASTUTININGSIH1 & EKO AGUS SUYONO2
1School of Life Sciences and Technology, Institut Teknologi Bandung, 40132 Bandung, Indonesia
2Faculty of Biology, Universitas Gadjah Mada, 55281 Yogyakarta, Indonesia
Diserahkan:
15 Januari 2020/Diserahkan:
15 April 2020
ABSTRACT
Chlorella vulgaris is a eukaryotic, unicellular green microalgae that can be harvested as protein source since it
can contain protein up to 50% of its dry weight. However, its cultivation is
costly due to the price of its growth medium. In this research we used bioslurry or known as anaerobically digested dairy manure wastewater
(ADDMW) as growth medium for C. vulgaris,
since dairy manure is known to contain high nitrogen and phosphorus and its
availability relatively abundant in rural areas. The cultivation of C.
vulgaris in the ADDMW medium was
conducted in lab-scale (19 L) photobioreactors. After
14 days the culture was able to produce chlorophyll content of 34.62 µg/mL, and
after 28 days was able to produce protein up to 35% dry weight. Moreover, C.
vulgaris was also able to reduce PO4-P,
NH4-N and NO3-N levels in ADDMW by 45.95, 78.24 and 17.38%, respectively.
Keywords:
ADDMW; Chlorella
vulgaris; chlorophyll; phytoremediation;
protein
ABSTRAK
Chlorella vulgaris adalah mikroalgae bersel tunggal yang memiliki kandungan protein sehingga 50% berat keringnya. Namun kos pengkulturan C. vulgaris adalah tinggi kerana medium pengkulturannya yang mahal. Dalam kajian ini,
kami menggunakan air buangan sapi cernaan anaerobik daripada proses penghasilan biogas. PengkulturanC.
vulgaris dalam medium air buangan sapi cernaan anaerobik (ADDMW) dalam fotobioreaktor (19 L) berskala makmal selama 14 hari dapat menghasilkanC.
vulgaris dengan kandungan klorofil 34.62
µg/mL dan dalam 28 hari dapat menghasilkan C. vulgaris dengan kadar protein 35% berat kering. Selain itu, kultur C. vulgaris dalam medium ADDMW dapat menurunkan kadar PO4-P, NH4-N dan NO3-N ADDMW masing-masing sebanyak 45.95, 78.24 dan 17.38%.
Kata kunci: ADDMW; Chlorella vulgaris; fitopemulihan; klorofil; protein
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