The Malaysian Journal of Analytical Sciences, Vol 13 No 1 (2009): 8 – 11

 

 

 

SIMULTANEOUS EXTRACTION AND CLEAN-UP

OF CHLORPYRIFOS FROM SPINACH (Spinacia oleracea) USING PRESSURISED LIQUID EXTRACTION (PLE)

 

Norashikin Saim, Rozita Osman*, Zaibunnisa Abdul Haiyee, Syafawati Mohd Salleh

 

Faculty of Applied Sciences, Universiti Teknologi MARA 40450 Shah Alam,

Selangor, Malaysia.

 

*Corresponding author: rozit471@salam.uitm.edu.my

 

 

Abstract

A simple one-step extraction and clean-up using pressurised liquid extraction (PLE) of chlorpyrifos from spinach (Spinacia oleracea) was developed. In this study, the effect of Florisil placed inside the extraction cell on recovery of chlorpyrifos and percentage bleachability of chlorophyll-a was investigated. Extractions were carried out using n-hexane and performed using these PLE conditions: extraction temperature of 100 C, pressure of 1500 psi and static extraction time of 10 min. Increase in the amount of Florisil  resulted in increase in the bleachability of chlorophyll a. It was found that 1.0 g Florisil was able to absorb 92.0% chlorophyll a without affecting the recovery of chlorpyrifos.

 

Keywords: spinach, pressurised liquid extraction, simultaneous extraction and clean-up

 

References

1.   Hussen, R. Westbom, N. Megersa, L. Mathiasson , Bjorklund, E. 2006. Development of a pressurised liquid extraction and clean-up procedure for the determination of α-endosulfan,   β-endosulfan and endosulfan sulphate in aged contaminated Ethiopian soils. Journal of Chromatography A. 1103, 202-210.

2.   Björklund, E., Sporring, S., Wiberg, K., Haglund, P. and Holst, C. 2006. New strategies for extraction and clean-up of persistent organic pollutants from food and feed samples using selective pressurised liquid extraction. Trends in Analytical Chemistry 25 (4), 318-325.

3.   Rodil, R. and Moeder, M. 2008. Development of a simultaneous pressurised-liquid extraction and clean-up procedure for the determination of UV filters in sediments. Analytica Chemica Acta 612 (2), 152-159.

4.   Omar, S., Girgis, B. &  Taha, F. 2003. Carbonaceous materials from seed hulls for bleaching of vegetable oils. Food Research International 36 (1): 11-17.

5.   Hussen, R. Westbom, N. Megersa, L. Mathiasson , Bjorklund, E. 2007. Selective pressurised liquid extraction for multi-residue analysis of organochlorine pesticides in soil. Journal of Chromatography A. 1160, 64-70.

6.   Official Methods of Analysis of AOAC International 2000. 17th Ed., AOAC INTERNATIONAL, Gaithersburg,  MD, USA.

7.   Mor, S., Ravindra, K., Bishnoi, N.R. 2007. Adsorption of chromium from aqueous solution by activated alumina and activated charcoal. Bioresource Technology 98: 954-957.