The Malaysian Journal of Analytical Sciences, Vol 13 No 1 (2009): 12 – 21
ANALISIS HASIL SINTESIS BERENZIM ESTER FRUKTOSA ASID LEMAK DARIPADA DISTILAT ASID LEMAK SAWIT DALAM MEDIUM TANPA PELARUT MENGGUNAKAN SPEKRUM RESONANS NUKLEUS
(Analysis of Products of Fructose-Fatty Acid Ester Enzymatic Synthesis from Palm Fatty Acid Distillate (PFAD) in Solvent-free Medium)
Mohd Wahid Samsuddin*, Mamot Said, Zurhana Husin, Halimahtun Zahrah Md Som
Pusat Pengajian Sains Kimia & Teknologi Makanan, Fakulti Sains & Teknologi UKM, 43600 Bangi, Selangor.
*Corresponding author: wahid@ukm.my
Abstract
A degradable nonionic surfactant was prepared from fructose and palm fatty acid distillate (PFAD) in solvent-free medium using immobilized Candida antartica Lipase (Novozyme 435) as a biocatalyst. Optimal parameters for the production of fructose fatty acid ester (FFAE) at 70 °C, molar ratio (fructose/ PFAD) 1:1, 8 % (w/w) lipase for 7 days of synthesis respectively.The separation of FFAE by column chromatography produced 2 types of esters, fructose monoester and fructose diester. Structure elucidation by 13C NMR confirmed that EFAL was a mixture of monoester isomers (α or β monoacylated at C1 or C6 of furanose and pyranose ring) and a mixture of diester isomers (α or β monoacylated at C1 and C6 of furanose ring). Purified FFAE contained fructose oleate as a major product with 19 % fructose monoester and 50.7 % fructose diester.
Keywords: Palm fatty acid distillate (PFAD), fructose, Novozyme 435, fructose fatty acid ester (FFAE)
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