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Sains Malaysiana 52(6)(2023):
http://doi.org/10.17576/jsm-2023-5206-05
Activity and
Safety of Cinchonine Nanostructured Lipid Carriers as a Hair Growth Stimulant
in Mice Model of Androgenetic Alopecia
(Aktiviti dan Keselamatan Pembawa Lipid Berstruktur
Nano Sinkonina sebagai Perangsang Pertumbuhan Rambut dalam Model Tikus Alopecia
Androgenetik)
HARIYANTI
HARIYANTI1,2, RACHMAT MAULUDIN1, YEYET CAHYATI
SUMIRTAPURA1 & NENG FISHERI KURNIATI3,*
1Department of Pharmaceutic,
School of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, 40132,
Indonesia
2Department of Pharmaceutical, Faculty of Pharmacy,
Universitas Muhammadiyah Purwokerto, Purwokerto, Central Java, 53182,
Indonesia
3Department of Pharmacology-Clinical Pharmacy, School
of Pharmacy, Institut Teknologi Bandung, Bandung, West Java, 40132, Indonesia
Diserahkan: 21 Februari 2022/Diterima: 9 Jun 2023
Abstract
Androgenetic
alopecia (AA) is a hair growth disorder characterized by hair loss and
miniaturization of the size of follicles and dermal papillae. Cinchonine is a
quinoline alkaloid that can increase VEGF production and potential as a hair
growth stimulant. This study aimed to determine the morphology, penetration (in
vitro), as well as safety and activity of cinchonine nanostructured lipid
carriers (CN-NLC) serum as a hair growth stimulant. Preparation of CN-NLC serum
using a combination of micro-emulsification and ultra-sonification methods,
characterization of CN-NLC serum included morphology and closed hair follicle
diffusion methods.
Dermal and eye irritation test using New Zealand rabbit strain with primary
irritation index parameters. Hair growth stimulant activity test using Swiss
Webster mice strain, induced with the hormone testosterone. Observations were
performed at 7, 14, and 21 days with hair length and histology parameters.
CN-NLC serum morphology is a spherical shape with size ±500 nm, diffusion of
serum CN-NLC (open hair follicles) of 23.92±0.84%; (close hair follicles) of
11.37±2.29%; and CN solution of 6.00±0.72%. CN-NLC serum are non-irritant with
a primary irritation index of 0.0. Activity tests showed hair length on days 7;
14 and 21 were increased by 20.24-23.74%; 33.47-36.43%, and 39.30-39.97%
(P≤0.05). Histological data showed an increase in the number and size of
both dermal papillae and hair follicles compared to the control group. CN-NLC
serum can improve the penetration of CN into hair follicles. CN-NLC serum is
safe and effective as a hair growth stimulant in the treatment of AA.
Keywords: Androgenic alopecia; cinchonine; nanostructured lipid
carriers
Abstrak
Alopecia
androgenetik (AA) adalah gangguan pertumbuhan rambut yang dicirikan oleh
keguguran rambut dan pengecilan saiz folikel dan papila dermal. Sinkonina
adalah alkaloid kuinolina yang boleh meningkatkan pengeluaran VEGF dan
berpotensi sebagai perangsang pertumbuhan rambut. Kajian ini bertujuan untuk
menentukan morfologi, penembusan (in vitro), serta keselamatan dan
aktiviti serum pembawa lipid berstruktur nano (CN-NLC) sinkonina sebagai
perangsang pertumbuhan rambut. Penyediaan serum CN-NLC menggunakan gabungan
kaedah pengemulsi mikro dan ultra-sonifikasi, pencirian serum CN-NLC termasuk
kaedah morfologi dan kaedah penyebaran folikel rambut tertutup. Ujian
kerengsaan kulit dan mata menggunakan ketegangan arnab New Zealand dengan
parameter indeks kerengsaan primer. Ujian aktiviti perangsang pertumbuhan
rambut menggunakan ketegangan tikus Swiss Webster, diinduksi dengan hormon
testosteron. Pemerhatian dilakukan pada 7, 14 dan 21 hari dengan panjang rambut
dan parameter histologi. Morfologi serum CN-NLC ialah bentuk sfera dengan saiz
±500 nm, resapan serum CN-NLC (folikel rambut terbuka) sebanyak 23.92±0.84%;
(folikel rambut tertutup) sebanyak 11.37±2.29%; dan larutan CN sebanyak
6.00±0.72%. Serum CN-NLC tidak merengsa dengan indeks kerengsaan primer 0.0.
Ujian aktiviti menunjukkan panjang rambut pada hari ke-7; 14 dan 21 meningkat
sebanyak 20.24-23.74%; 33.47-36.43% dan 39.30-39.97% (P≤0.05). Data
histologi menunjukkan peningkatan dalam bilangan dan saiz kedua-dua papila
dermal dan folikel rambut berbanding kumpulan kawalan. Serum CN-NLC dapat
meningkatkan penembusan CN ke dalam folikel rambut. Serum CN-NLC selamat dan
berkesan sebagai perangsang pertumbuhan rambut dalam rawatan AA.
Kata
kunci: Alopecia androgenetik; pembawa lipid berstruktur nano; sinkonina
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*Pengarang untuk surat-menyurat; email: nfkurniati@fa.itb.ac.id
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