Sains Malaysiana 45(11)(2016): 1675–1678

 

Biocompatibility of  TiO2 Nanorods and Nanoparticles on HeLa Cells

(Biokeserasian TiO2 Nanorod dan Nanopartikel ke atas Sel HeLa)

 

NOOR SAKINAH KHALID¹*, FATIN IZYANI MOHD FAZLI¹, NOOR KAMALIA ABD HAMED², MUHAMMAD LUQMAN MOHD NAPI², SOON CHIN FHONG³ & MOHD KHAIRUL AHMAD²

 

¹Faculty of Electrical and Electronic Engineering, Universiti Tun Hussein Onn Malaysia

86400 Parit Raja, Batu Pahat, Johor Darul Takzim, Malaysia

 

²Solar Device Research Laboratory, MiNT-SRC, UTHM, Universiti Tun Hussein Onn Malaysia

86400 Parit Raja, Batu Pahat, Johor Darul Takzim, Malaysia

 

³Biosensor and Bioengineering Labratory, MiNT-SRC, UTHM, Universiti Tun Hussein Onn Malaysia

86400 Parit Raja, Batu Pahat, Johor Darul Takzim, Malaysia

 

Received: 20 April 2015/Accepted: 12 October 2015

 

ABSTRACT

Titanium dioxide (TiO₂) nanorods and nanoparticles had been successfully done by hydrothermal method and spray pyrolysis deposition technique, respectively. Form XRD results, crystallite structure for TiO₂ nanorods is rutile phase at 2θ degree 27.5° which corresponded to [110] orientation. Whereas, TiO₂ nanoparticles produced anatase phase at 2θ degree 25.3° which corresponded to [110] plane. The structure of nanorods and nanoparticles were characterized using FESEM. The size of nanorods was in the range of 80 to 100 nm. While, the nanoparticles size was ranging from 25 to 35 nm. The HeLa cells were grown on those TiO2 and were observed under fluorescence microscope. The cells showed healthy sign of growth on TiO₂ nanorods and nanoparticles substrates. Thus, TiO₂ nanorods and nanoparticles are biocompatible to HeLa cells.

 

Keywords: Biocompatibility; nanoparticles; nanorods; TiO₂

 

ABSTRACT

Titanium dioksida (TiO₂) berbentuk nanorod dan nanopartikel telah berjaya dihasilkan melalui kaedah hidroterma dan teknik semburan pirolisis. Analisis daripada XRD menunjukkan komposisi untuk TiO₂ nanorod adalah fasa rutil pada 2θ darjah dengan bacaan 27.5° yang sepadan dengan jujukan [110]. Manakala nanopartikel TiO₂ adalah fasa anatas pada 2θ darjah dengan bacaan 25.3° yang sepadan dengan jujukan [101]. Bentuk nano-silinder dan nano-partikel telah dianalisis menggunakan FESEM. Saiz nanorod adalah antara 80 dan 100 nm diameter. Selain itu, saiz nano-partikel adalah 25 hingga 35 nm diameter. Kemudian, sel HeLa telah dibiakkan di atas sampel TiO₂ tersebut. Setelah itu, sel HeLa diperhatikan di bawah mikroskop pendarfluor. Keputusan menunjukkan pertumbuhan sel HeLa yang sihat pada TiO₂ nano-silinder dan nano-partikel. Oleh itu, TiO₂ nanorod dan nano-partikel menunjukkan keserasian biologi kepada sel HeLa.

 

Kata kunci: Keserasian biologi; nano-partikel; nano-silinder; TiO₂

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*Corresponding author; email: noorsakinahkhalid@gmail.com