Sains Malaysiana 47(10)(2018): 2391–2399

http://dx.doi.org/10.17576/jsm-2018-4710-15

 

Adhesion Ability and Cytotoxic Evaluation of Lactobacillus Strains Isolated from Malaysian Fermented Fish (Pekasam) on Ht-29 and Ccd-18Co Intestinal Cells

(Keupayaan Perlekatan dan Penilaian Sitotoksik Strain Lactobacillus Dipencilkan daripada Ikan Pekasam Malaysia ke atas Sel Usus Ht-29 dan Ccd-18Co)

 

IDA MURYANY¹, HING HIANG LIAN², INA-SALWANY^3,5, AHMAD ROHI GHAZALI¹, MOHD ZAMRI-SAAD⁴ & NOR FADILAH RAJAB^1*

 

¹Biomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

²Environmental Health Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia

 

³Aquaculture Department, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

⁴Veterinary Diagnostic Department, Faculty Veterinar Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

⁵School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia

 

Received: 22 February 2018/Accepted: 5 June 2018

 

ABSTRACT

 

Bacterial adhesion to host cells is the most important probiotic character. However, the adhesion of probiotic should not affect the viability of the host cells. In this study, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20 and Lactobacillus pentosus strain S1 were tested for their cytotoxic effects through MTT assay and their ability to adhere and colonize on HT-29 and CCD-18Co intestinal cells as detected microscopically using light microscopy and Scanning Electron Microscopy (SEM). No cytotoxicity effects were observed on both intestinal cells following 24 h treatment with all Lactobacillus strains. Additionally, all strains demonstrated strong adhesive activity where more than 100 bacteria adhered to both intestinal cells although differences in the adhesion scores observed among different strains. The adhesion as observed via SEM showed an autoagreggative pattern and adhered as clusters on the surface of both intestinal cells. In conclusion, all three Lactobacillus strains are non-cytotoxic to both cells with strong adhesion ability on intestinal cells and this study also proved that Malaysian fermented fish are good source of probiotic bacteria.

 

Keywords: Adhesion; Lactobacillus; probiotics; scanning electron microscopy (SEM)

 

ABSTRAK

 

Perlekatan bakteria pada sel perumah merupakan kriteria probiotik yang paling penting. Walau bagaimanapun, perlekatan probiotik mestilah tidak menjejaskan keviabelan sel perumah. Dalam kajian ini, Lactobacillus plantarum strain L8, Lactobacillus plantarum strain L20 dan Lactobacillus pentosus strain S1 telah diuji kesan sitotoksik ke atas sel usus melalui ujian MTT dan keupayaan mereka untuk melekat dan mengkoloni sel usus HT-29 dan CCD-18Co seperti yang dikesan secara mikroskopik menggunakan mikroskop cahaya dan pengimbasan mikroskop elektron (SEM). Keputusan kajian menunjukkan kesan sitotoksik tidak terdapat pada kedua sel usus berikut rawatan 24 jam dengan semua strain Lactobacillus. Selain itu, semua strain menunjukkan aktiviti perlekatan yang kuat untuk kedua sel usus walaupun terdapat perbezaan skor perlekatan pada strain yang berbeza. Paten perlekatan di bawah SEM menunjukkan corak autoagregatif dan melekat secara kluster pada permukaan kedua-dua sel usus. Kesimpulannya, kesemua strain Lactobacillus yang dikaji adalah tidak sitotoksik pada kedua sel dengan mempamerkan keupayaan perlekatan yang kuat pada sel usus.

 

Kata kunci: Lactobacillus; pengimbasan mikroskop elektron (SEM); perlekatan; probiotik

 

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*Corresponding author; email: nfadilah@ukm.edu.my