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Sains Malaysiana 47(10)(2018):
2401–2409 http://dx.doi.org/10.17576/jsm-2018-4710-16
Kesan Polifenol Minyak Kelapa Dara ke atas Aktiviti Enzim Antioksidan dan Lipid Peroksida Pada Sel Osteoblas (Virgin Coconut Oil Polyphenol Effects on Antioxidant Enzyme Activities and Lipid Peroxidation on Osteoblast
Cell) ZIL HAYATULLINA,
NORAZLINA
MOHAMED,
IMA-NIRWANA
SOELAIMAN
& NORLIZA MUHAMMAD* Jabatan Farmakologi,
Fakulti Perubatan,
Universiti Kebangsaan Malaysia,
50300
Kuala Lumpur, Wilayah Persekutuan, Malaysia Received:
3 April 2018/Accepted: 5 June 2018 ABSTRAK Osteoporosis
dikaitkan dengan
radikal bebas dan
tekanan oksidatif. Kandungan polifenol yang tinggi
dalam minyak
kelapa dara (VCO)
yang bertindak sebagai
antioksidan mampu menghalang tekanan oksidatif seterusnya mencegah osteoporosis. Uji kaji
in vitro ini dijalankan untuk mengkaji mekanisme kesan perlindungan polifenol ekstrak daripada VCO (PF)
ke atas sel
selanjar pre-osteoblas
mencit (MC3T3-E1) teraruh
tekanan oksidatif.
Untuk mengkaji viabiliti kesan perlindungan polifenol terhadap MC3T3-E1 daripada
toksisiti hidrogen
peroksida (H2O2),
sel MC3T3-E1 dirawat dengan beberapa dos polifenol selama 24 jam selepas dieram dengan 250 μM (IC50) H2O2 selama 1 jam. Mekanisme perlindungan dikaji dengan mengukur aras malondialdehid
(MDA)
serta aktiviti
enzim antioksidan iaitu superoksida dismutase (SOD),
glutation peroksidase
(GPx) dan katalase
(CAT).
Keputusan kajian
menunjukkan bahawa kepekatan perencatan median (IC50)
H2O2 pada 1 jam adalah 250 μM. Ujian viabiliti sel
mendapati kesemua dos polifenol dapat melindungi sel MC3T3-E1
daripada toksisiti
H2O2.
Dos 1 dan 0.01 μM
polifenol dapat meningkatkan aras
GPx dan CAT,
manakala dos 0.01 dan 0.0001 μM dapat meningkatkan
aras SOD. Sementara
itu, hanya
dos 0.01 dan 0.001 μM sahaja yang dapat menghalang peningkatan aras MDA.
Kesimpulan yang
dapat dibuat di dalam kajian ini
adalah polifenol
daripada minyak kelapa dara mencegah
lipid peroksida pada
sel MC3T3-E1 yang telah
diaruh dengan
H2O2 dengan meningkatkan aktiviti enzim antioksidan. Kata kunci: Osteoporosis; polifenol;
sel MC3T3-E1; tekanan oksidatif; VCO ABSTRACT Osteoporosis is associated
with free radicals and oxidative stress. The high polyphenol contents
in virgin coconut oil (VCO) can prevent oxidative stress
and consequently osteoporosis. This in vitro study was conducted in order to determine
the mechanism of the protective effects of polyphenol extracted
from VCO
on murine osteoblast-like cell line (MC3T3-E1)
which was induced with oxidative stress. To study the protective
effects on viability of MC3T3-E1 cells exposed
to hydrogen peroxide (H2O2)
toxicity, MC3T3-E1 cells were post-treated
with different doses of polyphenol for 24 h after 1 h incubation
with 250 μM of H2O2 (IC50).
The protective mechanism was studied by measuring melondialdehyde
(MDA)
levels as well as antioxidant enzymes activities which are superoxide
dismutase (SOD),
glutathione peroxidase (GPx) and catalase
(CAT).
The results showed that the median inhibitary
concentration (IC50) for H2O2 after
1 h was 250 μM. Cell viability
tests showed that all the doses of polyphenol were able to protect
MC3T3-E1 cells from H2O2 toxicity.
The dose of 1 and 0.01 μM of polyphenol
increased the level of GPx and CAT while
the dose of 0.01 and 0.0001 μM
increased the level of SOD. Meanwhile, the 0.0001 μM
of PF prevented the MDA elevation. In conclucion,
the polyphenol components in virgin coconut oil prevent lipid
peroxidation in MC3T3-E1 cells induced with
H2O2 by
increasing the endogenous antioxidant enzymes. Keywords: MC3T3-E1 cells; osteoporosis; oxidative
stress; polyphenol; VCO REFERENCES Abujazia, M.A.,
Norliza, M., Ahmad, N.S. & Ima Nirwana, S. 2012. The effects of virgin
coconut oil on bone oxidative status in ovariectomized
rats. Evidence-Based Complementary & Alternative
Medicine 2012: 525079. http:// dx.doi.org/10.1155/2012/525079.
Altindag, O.,
Erel, O., Soran,
N., Celik, H. & Selek, S. 2008.
Total oxidative/antioxidative status and
relation to bone mineral density in osteoporosis. Rheumatology
International 28(4): 317-321. Arora,
R., Chawla, R., Marwah, R., Arora, P.,
Sharma, R.K., Kaushik, V., Goel, R.,
Kaur, A., Silambarasan, M., Tripathi,
R.P. & J.R. Bhardwaj. 2011.
Potential of complementary and alternative medicine in preventive
management of novel H1N1 flu (Swine flu) pandemic: Thwarting potential
disasters in the Bud. Evidence-Based Complementary
& Alternative Medicine. 2011: 586506. doi:10.1155/2011/586506.
Baek,
K.H., Oh, K.W., Lee, W.Y., Lee, S.S., Kim, M.K., Kwon, H.S., Rhee,
E.J., Han J.H., Song, K.H. & Cha, B.Y. 2010. Association of
oxidative stress with postmenopausal osteoporosis and the effects
of hydrogen peroxide on osteoclast formation in human bone marrow
cell cultures. Calcified Tissue International
87(3): 226-235. Barnabé,
W., de Mendonça Neto,
T., Pimenta, F.C., Pegoraro, L.F. &
Scolaro, J.M. 2004. Efficacy
of sodium hypochlorite and coconut soap used as disinfecting agents
in the reduction of denture stomatitis, Streptococcus mutans
and Candida albicans. Journal
of Oral Rehabilitation 31(5): 453-459. Chodimella,
R., Anderson, J., Hong, Y. & Bishop, A. 2005. Nitric Oxide (NO) Sensitivity and Differentiation Status in Motor
Neurons (Abstract). Washington, DC: Society
for Neuroscience. Cosman,
F., de Beur, S.J., LeBoff,
M.S., Lewiecki, E.M., Tanner, B., Randall,
S. & Lindsay, R. 2014. Clinician’s guide to
prevention and treatment of osteoporosis. Osteoporosis
International 25(10): 2359-2381. Danielle,
A.C. & Jiang, J.X. 2015. Reactive oxygen
species and oxidative stress in osteoclastogenesis,
skeletal aging and bone diseases. Journal of Bone and
Mineral Metabolism 33(4): 359-370. Daroszewska,
A. 2015. Prevention and treatment of osteoporosis in women:
An update. Obstetrics, Gynaecology
& Reproductive Medicine 25(7): 181-187. Denisova,
N.A., Cantuti-Castelvetri, I., Hassan,
W.N., Paulson, K.E. & Joseph, J.A. 2001. Role of membrane lipids in regulation
of vulnerability to oxidative stress in PC12 cells: implication
for aging. Free Radical Biology and Medicine 30(6): 671-678.
Dosumu,
O.O., Duru, F.I.O., Osinuba,
A.A., Oremosu, A.A. & Noronha, C.C.
2010. Influence
of virgin coconut oil (VCNO) on oxidative stress, serum testosteron and gonadotropic hormones (FSH, LH) in chronic
ethanol ingestion. Agriculture & Biology Journal of North
America 1(6): 1126-1132. Fatokun,
A.A., Stone, T.W. & Smith, R.A. 2008. Responses of differentiated MC3T3-E1 osteoblastic cells to reactive
oxygen species. European Journal of Pharmacology 587(1-
3): 35-41. Hagiwara,
K., Goto, T., Araki, M., Miyazaki, H.
& Hagiwara, H. 2011. Olive polyphenol hydroxytyrosol
prevents bone loss. European Journal of Pharmacology 662(1-3):
78-84. Kamat,
J.P., Boloor, K.K., Devasagayam,
T.P.A. & Venkatachalam, S.R. 2000. Antioxidant properties of asparagus racemosus
against damage induced by gamma-radiation in rat liver mitochondria.
Journal of Ethnopharmacology 71(3):
425- 435. Lee, Y.S., Chen, X. & Anderson, J.J.B.
2001. Physiological concentrations of genistein
stimulate the proliferation and protect against free radical-induced
oxidative damage of MC3T3-E1 osteoblast-like cells. Nutrition
Research 21(9): 1287-1298. Linares,
G.R., Xing, W., Govoni, K.E., Chen,
S. & Mohan, S. 2009. Glutaredoxin
5 regulates osteoblast apoptosis ny
protecting against oxidative stress. Bone 44(5): 795-804. Maggio, D., Barabani, M., Pierandrei, M., Polidori, M.C., Catani, M., Mecocci,
P., Senin, U., Pacifici,
R. & Cherubini, A. 2003. Marked decrease in plasma
antioxidants in aged osteoporotic women: results of a cross-sectional
study. Journal of Clinical Endocrinology & Metabolism 88(4):
1523-1527. Manisha, D. & Shyamapada, M. 2011. Coconut (Cocos nucifera L.: Arecaceae): In health promotion and disease prevention. Asian
Pacific Journal of Tropical Medicine 4(3): 241-247. Marina, A.M., Che Man, Y.B. & Nazimah, A.H. 2009. Chemical properties
of virgin coconut oil. Journal of
the American Oil Chemists’ Society 86(4): 301-307.
McNamara, L.M. 2010. Perpective on post-menopausal osteoporosis: Establishing an
interdisciplinary understanding of the sequence of events from
the molecular level to whole bone fracktures.
Journal of the Royal Society Interface 7(44): 353-372.
Morton, D.J., Barrett-Connor, E.L. & Schneider, D.L. 2001. Vitamin C supplement use and bone mineral
density in postmenopausal women. Journal of Bone Mineral Research
16(1): 135-140. Muhammad, Z.M., Ahmad, N.S., Norazlina,
M., Norliza, M. & Ima-Nirwana,
S. 2010. Beneficial effects of vitamin E isomer supplementation
on static and dynamic bone histomorphometry
parameters in normal male rats. Journal of Bone and Mineral
Metabolism 28(5): 503-509. Nadia, M.E. & Ahmad, N.S. 2014. Time and dose-dependent effects of Labisia
pumila on bone oxidative status
of postmenopausal osteoporosis rat model. Nutrients
6(8): 3288-3302. Nevin, K.G. & Rajamohan, T. 2006. Virgin coconut oil supplemented diet increased the antioxidant status
in rats. Food Chemistry 99(2): 260-266. Nevin, K.G. & Rajamohan, T. 2008. Influence of virgin coconut oil on blood coagulation factors, lipid
levels and LDL oxidation in cholesterol fed Sprague-Dawley rats.
European Journal of Clinical Nutrition Metabolism 3: 1-8.
Nevin, K.G. & Rajamohan, T. 2005. Virgin coconut oil supplemented diet increases the antioxidant status
in rats. Food Chemistry 99(2): 260-266. Nevin, K.G. & Rajamohan, T. 2004. Beneficial effects of virgin coconut oil on lipid
parameters and in vitro LDL oxidation. Clinical
Biochemistry 37(9): 830-835. Nizar, A.M., Norazlina, M. & Ahmad,
N.S. 2012. Effects of low-dose versus high doses γ-tocotrienol
on the bone cells exposed to the hydrogen peroxide-induced oxidative
stress and apoptosis. Evidence-Based Complementary & Alternative
Medicine 2012: 680834. http://dx.doi. org/10.1155/2012/680834.
Norliza, M., Douglas, A.L., Ahmad,
N.S., Norazlina, M. & Ima-Nirwana,
S. 2012. Two different isomers of vitamin E prevent bone loss
in postmenopausal osteoporosis rat model. Evidence-Based Complementary
& Alternative Medicine 2012: 161527. https://doi.org/10.1155/2012/161527.
Oyi, A.R., Onaolapo, J.A. & Obi, R.C.
2010. Formulation and antimicrobial studies of coconut
(Cocos nucifera Linne)
oil. Research Journal of Applied Sciences, Engineering
and Technology 2(2): 133-137. Park, B.G., Yoo, C.I., Kim, H.T., Kwon,
C.H. & Kim, Y.K. 2005. Role of mitogen- activated
protein kinases in hydrogen peroxide-induced cell death in osteoblastic
cells. Toxicology 215(1-2): 115-125. Park, Y.H., Wan, D.W., Suh, H., Ryu, G.H.,
Hyon, S.H., Cho, B.K. & Park, J.C.
2003. Protective effects of green tea polyphenol against reactive
oxygen species-induced oxidative stress in cultures rat calvarial
osteoblast. Cell Biology & Toxicology 19(5): 325-337.
Pereira, R.M., Delany, A.M. & Canalis,
E. 2001. Cortisol inhibits the differentiation
and apoptosis of osteoblasts in culture. Bone 28(5): 484-490.
Rupesh, D., Chitrangada, A., Bindu, P.C. & Kundu, S.C. 2008. Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress
in skin fibroblasts. BMP Reports 41(3): 236-241.
Satoh, I., Sakai, N., Enokido, Y., Uchiyama,
Y. & Hatanaka, H. 1996. Free radical independent protection by nerve growth factor and Bcl-2
of PC12 cells from hydrogen peroxide-triggered apoptosis. Journal
of Biochemistry 120: 540-546. Scalbert, A., Johnson, I.T. & Saltmarsh, M. 2005. Polyphenols: Antioxidants and beyond. American Journal of Clinical
Nutrition 81(1): 215S-217S. Sendur, O.F., Turan, Y., Tastaban,
E. & Serter, M. 2009. Antioxidant status in patients with osteoporosis: A controlled study.
Joint Bone Spine 76(5): 514-518. Sosnowska, J. & Balslev, H. 2009. American palm ethnomedicine: A metaanalysis. Journal of Ethnobiology and Ethnomedicine 5: 43. https://doi.org/10.1186/1746-4269-5-43.
Taheri, J.B., Espineli, F.W., Lu, H., Asayesh, M., Bakshi, M. & Nakhostin, M.R. 2010. Antimicrobial
effect of coconut flour on oral microflora: An in vitro study.
Research Journal of Biological Sciences 5(6): 456-459.
Wang, Y.K., Hong, Y.J., Wei, M., Wu, Y., Huang, Z.Q., Chen, R.Z.
& Chen, H.Z. 2010. Curculigoside
attenuates human umbilicak vein endothelial
cell injury induced by H2O2.
Journal of Ethnopharmacology 132(1):
233-239. Wu, J.H., Xu, C., Shan, C.Y. & Tan, R.X. 2006. Antioxidant properties and PC12 cell protective effects of APS-1,
a polysaccharide from Aloe vera var.
Chinensis. Life Science 78(6): 622-630. Xu, Z.S., Wang, X.Y., Xiao, D.M., Hu, L.F., Lu, M., Wu, Z.Y. &
Bian, J.S. 2011. Hydrogen
sulfide protects MC3T3-E1 osteoblastic cells
against H2O2-induced oxidative damage -
implications for the treatmet of osteoporosis.
Free Radical Biology & Medicine 50(10): 1314-1323.
Yin, H., Xu, L. & Porter, N.A. 2011. Free radical lipid peroxidation: Mechanisms and analysis. Chemical
Reviews 111(10): 5944-5972. Zalavras, C., Shah, S., Birnbaum, M.J.
& Frenkel, B. 2003. Role
of apoptosis in glucocorticoid-induced osteoporosis and osteonecrosis.
Critical Reviews in Eukaryotic Gene Expression 13(2-4):
221-235. Zhang, J.K., Yang, L., Meng, G.L., Fang,
J., Chen, J.Z., He, Q.Z., Chen, S., Fan, J.Z., Luo, Z.J. &
Liu, J. 2012. Protective effects of tetrehydroxystilbene
glucoside against hydrogen peroxide-induced dysfunction and oxidative
stress in osteoblastic MC3T3-E1 cell.
European Journal of Pharmacology 689: 31-37. Zil Hayatullina,
Norliza, M., Norazlina,
M. & Ima-Nirwana, S. 2012. Virgin
coconut oil supplementation prevents bone loss in osteoporosis
rat model. Evidence-Based Complementary and Alternative Medicine
2012: 237236. *Corresponding author; email: norliza_ssp@ppukm.ukm.edu.my
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