Sains Malaysiana 47(10)(2018): 2411–2419

http://dx.doi.org/10.17576/jsm-2018-4710-17

 

Kesan Suplementasi Tokotrienol ke atas Perubahan Hormon dan Katekolamin Otak Tikus Aruhan Stres

(Effect of Tocotrienol Supplementation on Hormones and Catecholamines in the Brain of Rats Exposed to Stress)

 

NUR AZLINA MOHD FAHAMI^1*, NADDIAH SYAFIQAH RAMLI² & ELDA SURHAIDA LATIF²

 

¹Jabatan Farmakologi, Fakulti Perubatan, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

²Program Bioperubatan, Fakulti Sains Bersekutu, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

Received: 30 March 2018/Accepted: 5 June 2018

 

 

ABSTRAK

Stres merupakan sebarang gangguan, cabaran atau ancaman kepada seseorang individu yang merangsang perubahan respon fizikal, mental dan emosi. Hormon stres dan katekolamin dirembeskan di dalam otak sebagai respon terhadap stres. Kajian ini mengenal pasti kesan tokotrienol tulen dan vitamin E sawit ke atas perubahan hormon stres dan katekolamin pada tikus yang diaruhkan stres imobilisasi rendaman air. Sebanyak 32 ekor tikus Wistar jantan telah dibahagikan secara rawak kepada empat kumpulan kajian. Dua kumpulan kawalan; kumpulan tanpa stres (NS) dan kumpulan tanpa stres (CS) serta dua kumpulan rawatan yang diberikan tokotrienol (TTS) atau vitamin E sawit (TFS) secara oral paksaan pada dos 60 mg/kg berat badan selama 28 hari. Setelah tamat tempoh rawatan, tikus daripada kumpulan CS, TTS dan TFS telah didedahkan kepada stres imobilisasi rendaman air selama tiga setengan jam. Kemudian, otak tikus diambil untuk pengukuran hormon pelepasan kotikotrofin (CRH), hormon adenokortikotropik (ACTH), norepinefrin, dopamin dan serotonin. Keputusan kajian mendapati peningkatan kandungan CRH, ACTH, norepinefrin dan dopamine di dalam otak tikus yang diaruh stres berbanding kumpulan kawalan tanpa stres. Kandungan serotonin pula menurun akibat stres. Walau bagaimanapun, tikus yang diberi suplementasi tokotrienol dan vitamin E sawit menunjukkan penurunan signifikan kandungan CRH, ACTH, norepinefrin dan dopamin serta peningkatan kandungan dan serotonin sehingga hampir ke aras normal. Tiada perbezaan antara pemberian tokotrienol dan vitamin E sawit. Sebagai kesimpulan, tokotrienol dan vitamin E sawit berkesan dalam mengawal perubahan hormon stres serta katekolamin pada otak tikus yang teraruh stres.

 

Kata kunci: Hormon stres; katekolamin; otak; stres restrain; tokotrienol

 

ABSTRACT

Stress includes any disturbances, challenges or threats to an individual, which stimulates physical, mental and emotional responses. Stress hormones and catecholamines are released from the brain in response to stress. Antioxidant properties of vitamin E had been proven to help in reducing stress. This study was designed to investigate the effects of tocotrienol and palm vitamin E supplementation on stress hormones and catecholamines in stress-induced rats. Thirty two male Wistar rats were randomly assigned into four groups, consisted of two control groups (NS and CS) which were fed with a commercially prepared normal rat diet while two treatment groups (TTS and TFS) were given tocotrienol or palm vitamin E orally in the dose of 60 mg/kg body weight. After 28 days of treatment; CS, TTS and TFS rats were subjected to water-immersion restraint stress for three and a half hours once. The rats were killed and their brain was taken to determine stress hormones and catecholamines levels. The findings showed that stressed (CS) rats had a significantly higher levels of CRH, ACTH, norepinephrine and dopamine and lower level of serotonin in the brain. Interestingly, supplementation with both tocotrienol and palm vitamin E were able to significantly reduced the CRH, ACTH, norepinephrine and dopamine levels. No difference was observed when comparing the effects of tocotrienol with palm vitamin E. In conclusion, tocotrienol and palm vitamin E are capable in reducing changes to the stress hormones and catecholamines in stress-induced rats.

 

Keywords: Brain; catecholamines; palm vitamin E; restraint stress; stress hormones; tocotrienol

REFERENCES

Aggarwal, B.B., Sundarm, C., Prasad, S. & Kannapan, R. 2010. Tocotrienols, the vitamin E of the 21st century: Its potential against cancer and other chronic diseases. Biochemical Pharmacology 80(11): 1613-1631.

American Psychological Association. 2017. Stresses in America. New York: American Psycological Asociation.

Bardhan, J., Chakraborty, R. & Raychaudburi, U. 2011. The 21st century form of vitamin E-Tocotrienol. Current Pharmaceutical Design 17(21): 2196-2205.

Budin, S.B., Taib, I.S., Jayusman, P.A., Chiang, H.H., Ramalingam, A., Ghazali, A.R. & Mohamed, J. 2014. Ameliorative effect of palm oil tocotrienol rich fraction on brain oxidative stress in fenitrothion-administered rats. Sains Malaysiana 43(7): 1031-1036.

Cohen, G. 1987. Monoamine oxidase, hydeogen peroxide and Parkinson’s disease. Advances in Neurology 45: 119-125.

Cohen, G. & Spina, M.B. 1989. Deprenyl suppresses the oxidant stress associated with increased dopamine turnover. Annals of Neurology 26: 689-690.

Coppen, A. & Doogan, D. 1998. Serotonin and its place in the pathogenesis of depression. Journal of Clinical Psychiatry 21(2): 89-97.

Cowen, P.J. 2002. Cortisol, serotonin and depression: all stressed out? The British Journal of Psychiatry 180(2): 99-100.

Cowen, P.J. 2008. Serotonin and depression: Pathophysiology mechanism or marketing myth? Trends in Pharmacological Sciences 29(9): 433-436.

Finlay, J., Zigmoid, M. & Abercrombie, E. 1995. Increased dopamine and norepinephrine realease in medial prefrontal cortex induced by acute and chronic stress: Effect of diazepam. Neuroscience 64(3): 619-628.

Gamo, N.J., Lur, G., Higley, M.J., Wang, M., Paspalas, C.D., Vijayraghavan, S., Yang, Y., Ramos, B.P., Peng, K., Kata, A., Boven, L., Lin, F., Roman, L., Lee, D. & Arnsten, A.F.T. 2015. Stress impairs prefrontal cortical function via D1 Dopamin receptor interactions with hyperpolarization activated cyclic neucleotide-gates channels. Biological Psychiatry 78(12): 860-870.

Halliwell, B. 1996. Antioxidants in human health and disease. Annual Review of Nutrition 16: 33-50.

Halliwell, B. 2006. Reactive species and antioxidants: Redox biology is a fundamental theme of aerobic life. Plant Physiology 141(2): 312-322.

Hood, R.L. 1995. Tocotrienol and cholesterol metabolism. In Nutrition, Lipids, Health and Disease. Champaign, Illinois: AOCS Press. pp. 96-103.

Hsu, D.T., Lombrao, K.A., Bakshi, V.P., Balachandram, J.S., Roseboom, P.H. & Kalin, N.H. 2001. Acute stress-induced increases in thalamic CRH mRNA are blocked by repeated stress exposure. Brain Research 915(1): 18-24.

Ibrahim, A.A.I., Kamisah, Y., Nafeeza, M.I. & Nur Azlina, M.F. 2012. The effects of palm vitamin E on stress hormone levels and gastric lesions in stress induced rats. Archive of Medical Science 8(1): 22-29.

Lundberg, U. 2002. Psychophysiology of work: Stress, gender, endocrine response and work related upper extremity disorders. American Journal of Industrial Medicine 41(5): 383-392.

Mahar, I., Bambico, F.R., Mechawar, N. & Nobrega, N. 2014. Stress, serotonin and hippocampal neurogenesis in relation to depression and antidepressant effects. Neuroscience & Biobehavioral Reviews 38: 173-192.

Marinelli, M. & Piazza, P.V. 2002. Interaction between glucocorticoid hormones, stress and stress hormones on human cognition: Implications for the field of brain and cognition. Brain and Cognition 65(3): 209-237.

Nur Azlina, M.F., Kamisah, Y., Chua, K.H., Ibrahim, I.A.A. & Qodriyah, H.M.S. 2015. Preventive effects of tocotrienol on stress-induced gastric mucosal lesions and its relation to oxidative and inflammatory biomarkers. PloS One 10(10): e0139348.

Nur Azlina, M.F. & Nafeeza, M.I. 2008. Tocotrienol and a-tocopherol reduces corticosterone and noradrenalin levels in rats exposed to restraint stress. Pharmazie 63: 890-892.

Nur Azlina, M.F., Ibrahim, A.I., Kamisah, Y. & Nafeeza, M.I. 2012. Palm vitamin E reduces catecholamines, xanthine oxidase activity and gastric lesions in rats exposed to water-immersion restraint stress. BMC Gastroenterology 12: 54.

Penyedia pekerjaan global, Petunjuk ekonomi Global Regus. 2013.

Pongratz, G. & Straub, R.H. 2014. The sympathetic nervous response in inflammation. Arthritis Research & Therapy 16: 504.

Puglisi-Allegra, S. & Andolina, D. 2015. Serotonin and stress coping. Behavioural Brain Research 277: 58-67.

Romero, M.L. & Butler, L.K. 2007. Endocrinilogy of stress. International Journal of Comparative Psychology 20(2): 89-95.

Sakr, H.F., Abbas, A.M. & El Samanoudy, A.Z. 2015. Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats. Journal of Physiology and Pharmacology 66(2): 191-202.

Schiavone, S., Jaquet, V., Trabace, L. & Krause, K.H. 2013. Severe life stress and oxidative stres in the brain: From animal models to human pathology. Antioxidants & Redox Signaling 18(12): 1475-1490.

Serbinova, E.A. & Packer, L. 1994. Antioxidant and biological activities of palm-oil vitamin E. Food and Nutrition Bulletin- United Nations University 15: 138-138.

Taridi, N.M., Abd Rani, N., Abd Latiff, A., Wan Ngah, W.Z. & Mazlan, M. 2014. Tocotrienol rich fraction reverses age-related deficits in spatial learning and memory in aged rats. Lipids 49: 855.

Tort, L. & Teles, M. 2011. The endocrine resposes to stress: A coparative view. Dlm. Basic and Clinical Endocrinology Up-to Date, disunting oleh Akin, F. Croatia: Intech Europe. pp. 265-286.

Tsigos, C., Kyrou, I. & Kassi, E. 2000. Stress, endocrine physiology and pathophysiology. Dlm. Title of Book, disunting oleh De Groot, L.J., Chrousos, G., Dungan, K., Feingold, K.R., Grossman, A., Hershman, J.M., Koch, C., Korbonits, M., McLachlan, R., New, M., Purnell, J., Rebar, R., Singer, F. & Vinik, A. South Dartmouth (MA): MDText. com, Inc. https://www.ncbi.nlm.nih.gov/books/NBK278995/. Updated 2016, Mar 10.

Zaidi, S.M. & Banu, N. 2004. Antioxidant potential of vitamins A, E and C in modulating oxidative stres in rat brain. Clinica Chimica Acta 340(1-2): 229-233.

 

 

*Corresponding author; email: nurazlinamf@ukm. edu.my